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- W2956117636 endingPage "100275" @default.
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- W2956117636 abstract "Lignocellulose pretreatment with ionic liquids (IL) enables release of fermentable sugars at yields suitable for biofuel production. However, despite extensive washing, residual IL such as 1-ethyl-3-methylimidazolium acetate ([C2C1Im][OAc]) is harmful to microbes and enzymes involved in downstream processes. Ionic liquid-pretreated switchgrass hydrolysate contains from 0.2 to 4% (w/v) residual [C2C1Im][OAc] depending on the extent of water washing. A total of 25 yeast strains including four commercial ethanologenic Saccharomyces cerevisiae strains were tested to grow and ferment sugars in stepwise screening tests, and ethanol production was measured by GC-FID. Four yeast strains produced >10 g/L ethanol in laboratory media containing [C2C1Im][OAc]. The highest ethanol yield of 70% of theoretical yield was achieved by Wickerhamomyces anomalus UCDFST 72-248 when grown in hydrolysate with 3.2% residual IL. The robust growth and ethanol production by this wild (non-GMO) yeast, together with general stress tolerance of the species, suggests further exploration for biotechnology applications." @default.
- W2956117636 created "2019-07-12" @default.
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- W2956117636 date "2019-09-01" @default.
- W2956117636 modified "2023-09-24" @default.
- W2956117636 title "Ethanol production in switchgrass hydrolysate by ionic liquid-tolerant yeasts" @default.
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- W2956117636 doi "https://doi.org/10.1016/j.biteb.2019.100275" @default.
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