Matches in SemOpenAlex for { <https://semopenalex.org/work/W2956138959> ?p ?o ?g. }
- W2956138959 endingPage "3163" @default.
- W2956138959 startingPage "3163" @default.
- W2956138959 abstract "The production of soluble, functional recombinant proteins by engineered bacterial hosts is challenging. Natural molecular chaperone systems have been used to solubilize various recombinant proteins with limited success. Here, we attempted to facilitate chaperone-mediated folding by directing the molecular chaperones to their protein substrates before the co-translational folding process completed. To achieve this, we either anchored the bacterial chaperone DnaJ to the 3ʹ untranslated region of a target mRNA by fusing with an RNA-binding domain in the chaperone-recruiting mRNA scaffold (CRAS) system, or coupled the expression of DnaJ and a target recombinant protein using the overlapping stop-start codons 5ʹ-TAATG-3ʹ between the two genes in a chaperone-substrate co-localized expression (CLEX) system. By engineering the untranslated and intergenic sequences of the mRNA transcript, bacterial molecular chaperones are spatially constrained to the location of protein translation, expressing selected aggregation-prone proteins in their functionally active, soluble form. Our mRNA engineering methods surpassed the in-vivo solubilization efficiency of the simple DnaJ chaperone co-overexpression method, thus providing more effective tools for producing soluble therapeutic proteins and enzymes." @default.
- W2956138959 created "2019-07-12" @default.
- W2956138959 creator A5017735053 @default.
- W2956138959 creator A5024505376 @default.
- W2956138959 creator A5034329702 @default.
- W2956138959 creator A5054497310 @default.
- W2956138959 creator A5082154194 @default.
- W2956138959 creator A5086006406 @default.
- W2956138959 creator A5091067470 @default.
- W2956138959 date "2019-06-28" @default.
- W2956138959 modified "2023-10-07" @default.
- W2956138959 title "mRNA Engineering for the Efficient Chaperone-Mediated Co-Translational Folding of Recombinant Proteins in Escherichia coli" @default.
- W2956138959 cites W1498099361 @default.
- W2956138959 cites W1569669680 @default.
- W2956138959 cites W1693303585 @default.
- W2956138959 cites W191693931 @default.
- W2956138959 cites W1931534490 @default.
- W2956138959 cites W1964955754 @default.
- W2956138959 cites W1966162126 @default.
- W2956138959 cites W1970756887 @default.
- W2956138959 cites W1972522740 @default.
- W2956138959 cites W1973339647 @default.
- W2956138959 cites W1980890633 @default.
- W2956138959 cites W1985650639 @default.
- W2956138959 cites W1990869264 @default.
- W2956138959 cites W2003717601 @default.
- W2956138959 cites W2013429682 @default.
- W2956138959 cites W2015130768 @default.
- W2956138959 cites W2017313617 @default.
- W2956138959 cites W2019035215 @default.
- W2956138959 cites W2020496041 @default.
- W2956138959 cites W2021653782 @default.
- W2956138959 cites W2028840503 @default.
- W2956138959 cites W2029467366 @default.
- W2956138959 cites W2030671480 @default.
- W2956138959 cites W2031062414 @default.
- W2956138959 cites W2031456360 @default.
- W2956138959 cites W2034589999 @default.
- W2956138959 cites W2041914510 @default.
- W2956138959 cites W2043866745 @default.
- W2956138959 cites W2046955172 @default.
- W2956138959 cites W2047186187 @default.
- W2956138959 cites W2047360542 @default.
- W2956138959 cites W2052257078 @default.
- W2956138959 cites W2055468009 @default.
- W2956138959 cites W2057798220 @default.
- W2956138959 cites W2060945204 @default.
- W2956138959 cites W2063790877 @default.
- W2956138959 cites W2081435476 @default.
- W2956138959 cites W2083440806 @default.
- W2956138959 cites W2087961389 @default.
- W2956138959 cites W2091994760 @default.
- W2956138959 cites W2095939443 @default.
- W2956138959 cites W2096966529 @default.
- W2956138959 cites W2099540110 @default.
- W2956138959 cites W2105300484 @default.
- W2956138959 cites W2107334607 @default.
- W2956138959 cites W2109936231 @default.
- W2956138959 cites W2111517608 @default.
- W2956138959 cites W2111967267 @default.
- W2956138959 cites W2122500226 @default.
- W2956138959 cites W2127097337 @default.
- W2956138959 cites W2127612970 @default.
- W2956138959 cites W2128631050 @default.
- W2956138959 cites W2132207273 @default.
- W2956138959 cites W2135101596 @default.
- W2956138959 cites W2135337280 @default.
- W2956138959 cites W2135373808 @default.
- W2956138959 cites W2135431019 @default.
- W2956138959 cites W2136670217 @default.
- W2956138959 cites W2139816899 @default.
- W2956138959 cites W2141261198 @default.
- W2956138959 cites W2145123451 @default.
- W2956138959 cites W2153753900 @default.
- W2956138959 cites W2154106340 @default.
- W2956138959 cites W2156366212 @default.
- W2956138959 cites W2157296596 @default.
- W2956138959 cites W2159128192 @default.
- W2956138959 cites W2160373127 @default.
- W2956138959 cites W2164402735 @default.
- W2956138959 cites W2166726458 @default.
- W2956138959 cites W2167202196 @default.
- W2956138959 cites W2172170548 @default.
- W2956138959 cites W2184623063 @default.
- W2956138959 cites W2224160949 @default.
- W2956138959 cites W2571040143 @default.
- W2956138959 cites W2609563619 @default.
- W2956138959 cites W2735870257 @default.
- W2956138959 cites W2788148259 @default.
- W2956138959 cites W4238463126 @default.
- W2956138959 cites W979401529 @default.
- W2956138959 doi "https://doi.org/10.3390/ijms20133163" @default.
- W2956138959 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6651523" @default.
- W2956138959 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31261687" @default.
- W2956138959 hasPublicationYear "2019" @default.
- W2956138959 type Work @default.
- W2956138959 sameAs 2956138959 @default.
- W2956138959 citedByCount "3" @default.