Matches in SemOpenAlex for { <https://semopenalex.org/work/W2956142373> ?p ?o ?g. }
- W2956142373 endingPage "322" @default.
- W2956142373 startingPage "316" @default.
- W2956142373 abstract "Abstract Optimization of protein formulations at subzero temperatures is required for many applications such as storage, transport, and lyophilization. Using isochoric cooling (constant volume) is possible to reach subzero temperatures without freezing aqueous solutions. This accelerates protein damage as protein may unfold by cold denaturation and diffusional and conformational freedom is still present. The use of isochoric cooling to faster protein formulations was first demonstrated for the biomedical relevant protein disulfide isomerase A1. Three osmolytes, sucrose, glycerol, and l -arginine, significantly increased the stability of protein disulfide isomerase A1 at -20°C with all tested under isochoric cooling within the short time frame of 700 h. The redox green fluorescent protein 2 was used to evaluate the applicability of isochoric cooling for stability analysis of highly stable proteins. This derivative of GFP is 2.6-fold more stable than the highly stable GFP β-barrel structure. Nevertheless, it was possible to denature a fraction of roGFP2 at −20°C and to assign a stabilizing effect to sucrose. Isochoric cooling was further applied to insulin. Protein damage was evaluated through a signaling event elicited on human hepatocyte carcinoma cells. Insulin at −20°C under isochoric cooling lost 22% of its function after 15 days and 0.6M sucrose prevented insulin deactivation." @default.
- W2956142373 created "2019-07-12" @default.
- W2956142373 creator A5016475620 @default.
- W2956142373 creator A5028666503 @default.
- W2956142373 creator A5034208412 @default.
- W2956142373 creator A5061465268 @default.
- W2956142373 creator A5066475765 @default.
- W2956142373 creator A5072313196 @default.
- W2956142373 creator A5085929998 @default.
- W2956142373 creator A5087887712 @default.
- W2956142373 date "2020-01-01" @default.
- W2956142373 modified "2023-10-17" @default.
- W2956142373 title "Stability of Protein Formulations at Subzero Temperatures by Isochoric Cooling" @default.
- W2956142373 cites W1965302158 @default.
- W2956142373 cites W1965543425 @default.
- W2956142373 cites W1985616824 @default.
- W2956142373 cites W1995692089 @default.
- W2956142373 cites W1999120199 @default.
- W2956142373 cites W2013827851 @default.
- W2956142373 cites W2029446937 @default.
- W2956142373 cites W2036977073 @default.
- W2956142373 cites W2047976022 @default.
- W2956142373 cites W2050146234 @default.
- W2956142373 cites W2056866166 @default.
- W2956142373 cites W2057246411 @default.
- W2956142373 cites W2064275833 @default.
- W2956142373 cites W2069271483 @default.
- W2956142373 cites W2078221639 @default.
- W2956142373 cites W2081759583 @default.
- W2956142373 cites W2082746669 @default.
- W2956142373 cites W2083239064 @default.
- W2956142373 cites W2088873549 @default.
- W2956142373 cites W2089582113 @default.
- W2956142373 cites W2112645157 @default.
- W2956142373 cites W2128080180 @default.
- W2956142373 cites W2131268635 @default.
- W2956142373 cites W2157224473 @default.
- W2956142373 cites W2158585378 @default.
- W2956142373 cites W2187119809 @default.
- W2956142373 cites W2323587818 @default.
- W2956142373 cites W2603730074 @default.
- W2956142373 cites W2611081487 @default.
- W2956142373 cites W2753475057 @default.
- W2956142373 cites W2782280176 @default.
- W2956142373 cites W2884904187 @default.
- W2956142373 doi "https://doi.org/10.1016/j.xphs.2019.06.017" @default.
- W2956142373 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31255685" @default.
- W2956142373 hasPublicationYear "2020" @default.
- W2956142373 type Work @default.
- W2956142373 sameAs 2956142373 @default.
- W2956142373 citedByCount "13" @default.
- W2956142373 countsByYear W29561423732020 @default.
- W2956142373 countsByYear W29561423732021 @default.
- W2956142373 countsByYear W29561423732022 @default.
- W2956142373 countsByYear W29561423732023 @default.
- W2956142373 crossrefType "journal-article" @default.
- W2956142373 hasAuthorship W2956142373A5016475620 @default.
- W2956142373 hasAuthorship W2956142373A5028666503 @default.
- W2956142373 hasAuthorship W2956142373A5034208412 @default.
- W2956142373 hasAuthorship W2956142373A5061465268 @default.
- W2956142373 hasAuthorship W2956142373A5066475765 @default.
- W2956142373 hasAuthorship W2956142373A5072313196 @default.
- W2956142373 hasAuthorship W2956142373A5085929998 @default.
- W2956142373 hasAuthorship W2956142373A5087887712 @default.
- W2956142373 hasBestOaLocation W29561423731 @default.
- W2956142373 hasConcept C121332964 @default.
- W2956142373 hasConcept C185592680 @default.
- W2956142373 hasConcept C43617362 @default.
- W2956142373 hasConcept C91556481 @default.
- W2956142373 hasConcept C97355855 @default.
- W2956142373 hasConceptScore W2956142373C121332964 @default.
- W2956142373 hasConceptScore W2956142373C185592680 @default.
- W2956142373 hasConceptScore W2956142373C43617362 @default.
- W2956142373 hasConceptScore W2956142373C91556481 @default.
- W2956142373 hasConceptScore W2956142373C97355855 @default.
- W2956142373 hasFunder F4320320273 @default.
- W2956142373 hasFunder F4320320300 @default.
- W2956142373 hasFunder F4320334779 @default.
- W2956142373 hasFunder F4320335226 @default.
- W2956142373 hasFunder F4320335322 @default.
- W2956142373 hasIssue "1" @default.
- W2956142373 hasLocation W29561423731 @default.
- W2956142373 hasOpenAccess W2956142373 @default.
- W2956142373 hasPrimaryLocation W29561423731 @default.
- W2956142373 hasRelatedWork W1531601525 @default.
- W2956142373 hasRelatedWork W1990781990 @default.
- W2956142373 hasRelatedWork W2085475542 @default.
- W2956142373 hasRelatedWork W2109773298 @default.
- W2956142373 hasRelatedWork W2606768298 @default.
- W2956142373 hasRelatedWork W2606769243 @default.
- W2956142373 hasRelatedWork W2748952813 @default.
- W2956142373 hasRelatedWork W2899084033 @default.
- W2956142373 hasRelatedWork W2948807893 @default.
- W2956142373 hasRelatedWork W2778153218 @default.
- W2956142373 hasVolume "109" @default.
- W2956142373 isParatext "false" @default.
- W2956142373 isRetracted "false" @default.
- W2956142373 magId "2956142373" @default.