FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2956603010> ?p ?o ?g. }

• W2956603010 abstract "Genetically modified mice are indispensable for establishing the roles of platelets in arterial thrombosis and hemostasis. Microfluidics assays using anticoagulated whole blood are commonly used as integrative proxy tests for platelet function in mice. In the present study, we quantified the changes in collagen-dependent thrombus formation for 38 different strains of (genetically) modified mice, all measured with the same microfluidics chamber. The mice included were deficient in platelet receptors, protein kinases or phosphatases, small GTPases or other signaling or scaffold proteins. By standardized re-analysis of high-resolution microscopic images, detailed information was obtained on altered platelet adhesion, aggregation and/or activation. For a subset of 11 mouse strains, these platelet functions were further evaluated in rhodocytin- and laminin-dependent thrombus formation, thus allowing a comparison of glycoprotein VI (GPVI), C-type lectin-like receptor 2 (CLEC2) and integrin 61 pathways. High homogeneity was found between wild-type mice datasets concerning adhesion and aggregation parameters. Quantitative comparison for the 38 modified mouse strains resulted in a matrix visualizing the impact of the respective (genetic) deficiency on thrombus formation with detailed insight into the type and extent of altered thrombus signatures. Network analysis revealed strong clusters of genes involved in GPVI signaling and Ca2+ homeostasis. The majority of mice demonstrating an antithrombotic phenotype in vivo displayed with a larger or smaller reduction in multi-parameter analysis of collagen-dependent thrombus formation in vitro. Remarkably, in only approximately half of the mouse strains that displayed reduced arterial thrombosis in vivo, this was accompanied by impaired hemostasis. This was also reflected by comparing in vitro thrombus formation (by microfluidics) with alterations in in vivo bleeding time. In conclusion, the presently developed multi-parameter analysis of thrombus formation using microfluidics can be used to: (i) determine the severity of platelet abnormalities; (ii) distinguish between altered platelet adhesion, aggregation and activation; and (iii) elucidate both collagen and non-collagen dependent alterations of thrombus formation. This approach may thereby aid in the better understanding and better assessment of genetic variation that affect in vivo arterial thrombosis and hemostasis." @default.
• W2956603010 created "2019-07-23" @default.
• W2956603010 creator A5000171105 @default.
• W2956603010 creator A5001017179 @default.
• W2956603010 creator A5001883769 @default.
• W2956603010 creator A5004295225 @default.
• W2956603010 creator A5007166561 @default.
• W2956603010 creator A5008888080 @default.
• W2956603010 creator A5013838935 @default.
• W2956603010 creator A5014366123 @default.
• W2956603010 creator A5035254895 @default.
• W2956603010 creator A5037465236 @default.
• W2956603010 creator A5044474574 @default.
• W2956603010 creator A5047569756 @default.
• W2956603010 creator A5051027118 @default.
• W2956603010 creator A5054090129 @default.
• W2956603010 creator A5055949749 @default.
• W2956603010 creator A5066251274 @default.
• W2956603010 creator A5068782139 @default.
• W2956603010 creator A5079584981 @default.
• W2956603010 creator A5083653940 @default.
• W2956603010 creator A5089140495 @default.
• W2956603010 creator A5091194864 @default.
• W2956603010 date "2019-07-30" @default.
• W2956603010 modified "2023-10-14" @default.
• W2956603010 title "Comparative Analysis of Microfluidics Thrombus Formation in Multiple Genetically Modified Mice: Link to Thrombosis and Hemostasis" @default.
• W2956603010 cites W1513172031 @default.
• W2956603010 cites W1530702942 @default.
• W2956603010 cites W1550488846 @default.
• W2956603010 cites W1657825487 @default.
• W2956603010 cites W1717627516 @default.
• W2956603010 cites W1968066168 @default.
• W2956603010 cites W2001031266 @default.
• W2956603010 cites W2005464779 @default.
• W2956603010 cites W2017800734 @default.
• W2956603010 cites W2029684068 @default.
• W2956603010 cites W2032243899 @default.
• W2956603010 cites W2041777103 @default.
• W2956603010 cites W2044947105 @default.
• W2956603010 cites W2047144211 @default.
• W2956603010 cites W2048896125 @default.
• W2956603010 cites W2053305824 @default.
• W2956603010 cites W2053521976 @default.
• W2956603010 cites W2058742122 @default.
• W2956603010 cites W2062053430 @default.
• W2956603010 cites W2065167665 @default.
• W2956603010 cites W2069047064 @default.
• W2956603010 cites W2070368409 @default.
• W2956603010 cites W2075730871 @default.
• W2956603010 cites W2077584046 @default.
• W2956603010 cites W2087411946 @default.
• W2956603010 cites W2090642301 @default.
• W2956603010 cites W2091437674 @default.
• W2956603010 cites W2094652341 @default.
• W2956603010 cites W2096173332 @default.
• W2956603010 cites W2100261477 @default.
• W2956603010 cites W2111485605 @default.
• W2956603010 cites W2118656679 @default.
• W2956603010 cites W2121476395 @default.
• W2956603010 cites W2122082665 @default.
• W2956603010 cites W2128541973 @default.
• W2956603010 cites W2129186454 @default.
• W2956603010 cites W2130083671 @default.
• W2956603010 cites W2133686229 @default.
• W2956603010 cites W2136057525 @default.
• W2956603010 cites W2136850043 @default.
• W2956603010 cites W2139448535 @default.
• W2956603010 cites W2141088469 @default.
• W2956603010 cites W2149570553 @default.
• W2956603010 cites W2152955882 @default.
• W2956603010 cites W2159675211 @default.
• W2956603010 cites W2163401883 @default.
• W2956603010 cites W2167279371 @default.
• W2956603010 cites W2260737152 @default.
• W2956603010 cites W2284815995 @default.
• W2956603010 cites W2604840313 @default.
• W2956603010 cites W2610786733 @default.
• W2956603010 cites W2736021082 @default.
• W2956603010 cites W2758170836 @default.
• W2956603010 cites W2767490506 @default.
• W2956603010 cites W2771736938 @default.
• W2956603010 cites W2781650509 @default.
• W2956603010 cites W2792943263 @default.
• W2956603010 cites W2808534085 @default.
• W2956603010 cites W2895685061 @default.
• W2956603010 cites W2901813557 @default.
• W2956603010 cites W2903951623 @default.
• W2956603010 cites W4247741685 @default.
• W2956603010 cites W4249606224 @default.
• W2956603010 cites W747719605 @default.
• W2956603010 doi "https://doi.org/10.3389/fcvm.2019.00099" @default.
• W2956603010 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6682619" @default.
• W2956603010 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31417909" @default.
• W2956603010 hasPublicationYear "2019" @default.
• W2956603010 type Work @default.
• W2956603010 sameAs 2956603010 @default.
• W2956603010 citedByCount "9" @default.
• W2956603010 countsByYear W29566030102019 @default.
• W2956603010 countsByYear W29566030102020 @default.
• W2956603010 countsByYear W29566030102021 @default.

• next