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- W2956663546 abstract "A novel backcross variety (BC) [Megalobrama amblycephala (MA) ♀ × (Megalobrama amblycephala ♀ × Culter alburnus (CA) ♂) ♂] was generated by artificial insemination. The BC exhibits growth superiority and enhanced digestive enzyme activity than that of its parents. We conducted the transcriptome analysis using hepatopancreas between the BC and its parents. Three hundred and twenty-five and 872 differentially expressed genes (DEGs) were identified in ‘BC vs. MA’ and ‘BC vs. CA’, respectively. The GO and KEGG enrichment analysis of DEGs were classified. From the 134 overlapping DEGs and their pathway, the digestive enzyme genes, such as TRY, ELA1, CTRL1, CPA2, and BAL, and the IGF system genes, such as IGF1, IGF2a, and IGFBP2b, of the BC had significant difference in expression. Furthermore, the expression of downstream genes related to the protein and fatty acid synthesis pathways, such as PI3KR, RAPTOR, EIF4E, CS, MDH, FAS, ELOVL1, ELOVL5, and ELOVL6, were significantly up-regulated. In addition, a total of 40,732 SSRs and 52,951 SNPs were identified from the transcriptome. The results revealed that the enhanced digestive enzymes activity and synthesis of proteins/fatty acids might be contributed to growth superiority of backcross BC." @default.
- W2956663546 created "2019-07-23" @default.
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- W2956663546 date "2019-10-01" @default.
- W2956663546 modified "2023-09-30" @default.
- W2956663546 title "Transcriptome analysis provides new insights into the growth superiority of a novel backcross variety, Megalobrama amblycephala ♀ × (M. amblycephala ♀ × Culter alburnus ♂) ♂" @default.
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- W2956663546 doi "https://doi.org/10.1016/j.aquaculture.2019.734317" @default.
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