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- W2956933622 abstract "Shipworms are marine bivalve molluscs, known for their wood boring abilities. They use modified shells to bore into and grind wood, which is then digested. The shipworm’s ability to feed on lignocellulose is dependent on the presence of endosymbiotic bacteria that live in the animal’s gills inside specialized eukaryotic cells called bacteriocytes. These bacteria provide the animal with hydrolytic enzymes for wood digestion, which are translocated from the gills to the caecum, the main site of wood digestion. Unlike other lignocellulose degrading organisms, which harbour symbiotic microbes in their digestive tract, the shipworm caecum hosts only few bacteria but contains a large amount of carbohydrate active enzymes (CAZymes) of both endogenous and bacterial origin.This study investigates the anatomical, physiological and molecular basis of wood digestion in the shipworm Lyrodus pedicellatus. A combination of meta-transcriptomics, meta-proteomics and microscopic studies of the shipworm digestive organs were used, coupled with recombinant production and characterisation of some of the most expressed lignocellulolytic enzymes. This multidisciplinary analysis revealed how two structures, the food groove (a mucus stream utilised by filter feeding molluscs to transport food particles from the gills to the digestive system) and the crystalline style (a rotating structure hosted in the stomach involved in extra-cellular digestion) have been co-opted in shipworms to translocate bacteria and their enzymes from the gills to the caecum, to facilitate wood digestion. The transcriptomic and proteomic results indicate that bacterial lignocellulolytic enzymes are expressed in the gills, while the endogenous enzymes are mainly produced by the digestive glands, with complementary CAZy classes being expressed by the bacteria and the shipworms, indicating a subdivision of roles. Five bacterial CAZymes were recombinantly expressed and characterised, showing activity on cellulose, galacto-glucomannan and xylan. This study provides new insights into the mechanisms of wood digestion in shipworms, which may have biotechnological relevance." @default.
- W2956933622 created "2019-07-23" @default.
- W2956933622 creator A5001493968 @default.
- W2956933622 date "2018-09-30" @default.
- W2956933622 modified "2023-09-23" @default.
- W2956933622 title "Physiology and enzymology of lignocellulose digestion in the shipworm Lyrodus pedicellatus" @default.
- W2956933622 cites W103892340 @default.
- W2956933622 cites W1159215848 @default.
- W2956933622 cites W1480116128 @default.
- W2956933622 cites W1541957014 @default.
- W2956933622 cites W1544279297 @default.
- W2956933622 cites W1558215867 @default.
- W2956933622 cites W1561750927 @default.
- W2956933622 cites W1563330113 @default.
- W2956933622 cites W1565034742 @default.
- W2956933622 cites W1569993650 @default.
- W2956933622 cites W1579819643 @default.
- W2956933622 cites W1590518751 @default.
- W2956933622 cites W1611625941 @default.
- W2956933622 cites W1644953120 @default.
- W2956933622 cites W1672659680 @default.
- W2956933622 cites W1788645855 @default.
- W2956933622 cites W1793956901 @default.
- W2956933622 cites W1834683107 @default.
- W2956933622 cites W1849936841 @default.
- W2956933622 cites W1869516088 @default.
- W2956933622 cites W18751906 @default.
- W2956933622 cites W1932277571 @default.
- W2956933622 cites W1941184330 @default.
- W2956933622 cites W1962539204 @default.
- W2956933622 cites W1964106002 @default.
- W2956933622 cites W1965034600 @default.
- W2956933622 cites W1965870364 @default.
- W2956933622 cites W1965879930 @default.
- W2956933622 cites W1966692598 @default.
- W2956933622 cites W1966775748 @default.
- W2956933622 cites W1967215617 @default.
- W2956933622 cites W1967796434 @default.
- W2956933622 cites W1969080446 @default.
- W2956933622 cites W1970458175 @default.
- W2956933622 cites W1970460766 @default.
- W2956933622 cites W1970642585 @default.
- W2956933622 cites W1971463219 @default.
- W2956933622 cites W1971769148 @default.
- W2956933622 cites W1972222351 @default.
- W2956933622 cites W1972465707 @default.
- W2956933622 cites W1973717902 @default.
- W2956933622 cites W1975410303 @default.
- W2956933622 cites W1975796937 @default.
- W2956933622 cites W1975914194 @default.
- W2956933622 cites W1976614981 @default.
- W2956933622 cites W1976804099 @default.
- W2956933622 cites W1977423968 @default.
- W2956933622 cites W1978437683 @default.
- W2956933622 cites W1979194637 @default.
- W2956933622 cites W1980548993 @default.
- W2956933622 cites W1980701408 @default.
- W2956933622 cites W1981276913 @default.
- W2956933622 cites W1983140978 @default.
- W2956933622 cites W1985354366 @default.
- W2956933622 cites W1985896531 @default.
- W2956933622 cites W1986576915 @default.
- W2956933622 cites W1988266865 @default.
- W2956933622 cites W1989080603 @default.
- W2956933622 cites W1989827032 @default.
- W2956933622 cites W1990150001 @default.
- W2956933622 cites W1991372594 @default.
- W2956933622 cites W1991486978 @default.
- W2956933622 cites W1992066825 @default.
- W2956933622 cites W1992421280 @default.
- W2956933622 cites W1992480188 @default.
- W2956933622 cites W1992613244 @default.
- W2956933622 cites W1992832656 @default.
- W2956933622 cites W1993259241 @default.
- W2956933622 cites W1993510136 @default.
- W2956933622 cites W1993735700 @default.
- W2956933622 cites W1993810647 @default.
- W2956933622 cites W1993940896 @default.
- W2956933622 cites W1996442036 @default.
- W2956933622 cites W1996927050 @default.
- W2956933622 cites W1998002229 @default.
- W2956933622 cites W2000806044 @default.
- W2956933622 cites W2000872373 @default.
- W2956933622 cites W2001076707 @default.
- W2956933622 cites W2002538371 @default.
- W2956933622 cites W2003507059 @default.
- W2956933622 cites W2006440249 @default.
- W2956933622 cites W2007360988 @default.
- W2956933622 cites W2007764832 @default.
- W2956933622 cites W2008497859 @default.
- W2956933622 cites W2008546676 @default.
- W2956933622 cites W2008610881 @default.
- W2956933622 cites W2009555092 @default.
- W2956933622 cites W2009572716 @default.
- W2956933622 cites W2009674410 @default.
- W2956933622 cites W2010581670 @default.
- W2956933622 cites W2011301426 @default.
- W2956933622 cites W2013569936 @default.
- W2956933622 cites W2015351139 @default.
- W2956933622 cites W2016172247 @default.