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- W2957838052 abstract "Abstract EDTA‐capped iron oxide nanoparticles (Fe 3 O 4 / edta NPs) were synthesized by simple wet chemical method and encapsulated with amphiphilic triblock copolymer Pluronic ® F127 (Fe 3 O 4 / edta/P) . The resulting ‘core‐corona’ system becomes capable of condensing plasmid DNA (pDNA) and act as vehicle for cell transformation. As a proof‐of‐concept, E.coli_DH5α bacterial strain was selected. The plasmids like pBSKS and pUC18 having antibiotic resistance marker gene were eluted from the bacterial strain, purified and loaded on the synthesized nano‐assembly. Green fluorescence protein tag was attached to pTX (pTX gfp ) to confirm successful transformation. The gene transformation efficiency (TE) of the developed Fe 3 O 4 / edta / P vehicle was compared with traditional CaCl 2 mediated transformation as a positive control and naked pTX gfp was used as a negative control. PEGlation of Fe 3 O 4 / edta was also carried out and the TE of the resulting system was compared with as‐synthesized Fe 3 O 4 / edta/P . This study demonstrates that Pluronic ® F127 polymeric surfactant can be used to form stealth surface of the nanocarrier to solve Polyethylene Glycol (PEG) dilemma. The probable mechanism to explain the bacterial cell transformation is discussed." @default.
- W2957838052 created "2019-07-23" @default.
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- W2957838052 date "2019-07-17" @default.
- W2957838052 modified "2023-10-14" @default.
- W2957838052 title "EDTA‐Capped Iron Oxide Core‐Corona System as Vehicle for Gene Delivery to Transform <i>E.coli</i> : Mimicking the Lipid Bilayer Environment" @default.
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- W2957838052 doi "https://doi.org/10.1002/slct.201900964" @default.
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