FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2958604821> ?p ?o ?g. }

• W2958604821 endingPage "e0219487" @default.
• W2958604821 startingPage "e0219487" @default.
• W2958604821 abstract "Erwinia uzenensis is a plant-pathogenic bacterium, recently described in Japan, which infects pear trees, causing the 'bacterial black shoot disease of European pear' (BBSDP). Like other Erwinia pear pathogens, E. uzenensis causes damp, black lesions on young shoots resembling those of E. amylovora, but not blossom blight, fruitlet blight or wilting of the shoot tip. The distribution of E. uzenensis seems restricted to the country where it was reported up to now, but it may spread to other countries and affect new hosts, as is the current situation with E. piriflorinigrans and E. pyrifoliae. Fast and accurate detection systems for this new pathogen are needed to study its biology and to identify it on pear or other hosts. We report here the development of a specific and sensitive detection protocol based on a real-time PCR with a TaqMan probe for E. uzenensis, and its evaluation. In sensitivity assays, the detection threshold of this protocol was 101 cfu ml-1 on pure bacterial cultures and 102-103 cfu ml-1 on spiked plant material. The specificity of the protocol was evaluated against E. uzenensis and 46 strains of pear-associated Erwinia species different to E. uzenensis. No cross-reaction with the non-target bacterial species or the loss of sensitivity were observed. This specific and sensitive diagnostic tool may reveal a wider distribution and host range of E. uzenensis initially considered restricted to a region and will expand our knowledge of the life cycle and environmental preferences of this pathogen." @default.
• W2958604821 created "2019-07-23" @default.
• W2958604821 creator A5028464985 @default.
• W2958604821 creator A5043212744 @default.
• W2958604821 creator A5046145595 @default.
• W2958604821 creator A5067947166 @default.
• W2958604821 creator A5069205845 @default.
• W2958604821 creator A5075606251 @default.
• W2958604821 date "2019-07-10" @default.
• W2958604821 modified "2023-10-16" @default.
• W2958604821 title "Development of a real-time PCR method for the specific detection of the novel pear pathogen Erwinia uzenensis" @default.
• W2958604821 cites W1483304337 @default.
• W2958604821 cites W1575103257 @default.
• W2958604821 cites W1586528492 @default.
• W2958604821 cites W1763493835 @default.
• W2958604821 cites W1964489543 @default.
• W2958604821 cites W1971512222 @default.
• W2958604821 cites W1979201554 @default.
• W2958604821 cites W1984164827 @default.
• W2958604821 cites W2001126099 @default.
• W2958604821 cites W2014772310 @default.
• W2958604821 cites W2018635688 @default.
• W2958604821 cites W2029897412 @default.
• W2958604821 cites W2033512785 @default.
• W2958604821 cites W2033713984 @default.
• W2958604821 cites W2045843097 @default.
• W2958604821 cites W2046753373 @default.
• W2958604821 cites W2046938041 @default.
• W2958604821 cites W2058422951 @default.
• W2958604821 cites W2086219374 @default.
• W2958604821 cites W2086866565 @default.
• W2958604821 cites W2096476572 @default.
• W2958604821 cites W2097124701 @default.
• W2958604821 cites W2100408063 @default.
• W2958604821 cites W2102223242 @default.
• W2958604821 cites W2106882534 @default.
• W2958604821 cites W2139301713 @default.
• W2958604821 cites W2164959864 @default.
• W2958604821 cites W2279753633 @default.
• W2958604821 cites W2590907424 @default.
• W2958604821 cites W4214676503 @default.
• W2958604821 cites W916541636 @default.
• W2958604821 doi "https://doi.org/10.1371/journal.pone.0219487" @default.
• W2958604821 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6619794" @default.
• W2958604821 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31291321" @default.
• W2958604821 hasPublicationYear "2019" @default.
• W2958604821 type Work @default.
• W2958604821 sameAs 2958604821 @default.
• W2958604821 citedByCount "4" @default.
• W2958604821 countsByYear W29586048212020 @default.
• W2958604821 countsByYear W29586048212021 @default.
• W2958604821 countsByYear W29586048212022 @default.
• W2958604821 crossrefType "journal-article" @default.
• W2958604821 hasAuthorship W2958604821A5028464985 @default.
• W2958604821 hasAuthorship W2958604821A5043212744 @default.
• W2958604821 hasAuthorship W2958604821A5046145595 @default.
• W2958604821 hasAuthorship W2958604821A5067947166 @default.
• W2958604821 hasAuthorship W2958604821A5069205845 @default.
• W2958604821 hasAuthorship W2958604821A5075606251 @default.
• W2958604821 hasBestOaLocation W29586048211 @default.
• W2958604821 hasConcept C104317684 @default.
• W2958604821 hasConcept C143660080 @default.
• W2958604821 hasConcept C144027150 @default.
• W2958604821 hasConcept C21410773 @default.
• W2958604821 hasConcept C2775874295 @default.
• W2958604821 hasConcept C2776460866 @default.
• W2958604821 hasConcept C2776496690 @default.
• W2958604821 hasConcept C2777845778 @default.
• W2958604821 hasConcept C2778495000 @default.
• W2958604821 hasConcept C48023723 @default.
• W2958604821 hasConcept C523546767 @default.
• W2958604821 hasConcept C54355233 @default.
• W2958604821 hasConcept C86803240 @default.
• W2958604821 hasConcept C89423630 @default.
• W2958604821 hasConcept C90355303 @default.
• W2958604821 hasConceptScore W2958604821C104317684 @default.
• W2958604821 hasConceptScore W2958604821C143660080 @default.
• W2958604821 hasConceptScore W2958604821C144027150 @default.
• W2958604821 hasConceptScore W2958604821C21410773 @default.
• W2958604821 hasConceptScore W2958604821C2775874295 @default.
• W2958604821 hasConceptScore W2958604821C2776460866 @default.
• W2958604821 hasConceptScore W2958604821C2776496690 @default.
• W2958604821 hasConceptScore W2958604821C2777845778 @default.
• W2958604821 hasConceptScore W2958604821C2778495000 @default.
• W2958604821 hasConceptScore W2958604821C48023723 @default.
• W2958604821 hasConceptScore W2958604821C523546767 @default.
• W2958604821 hasConceptScore W2958604821C54355233 @default.
• W2958604821 hasConceptScore W2958604821C86803240 @default.
• W2958604821 hasConceptScore W2958604821C89423630 @default.
• W2958604821 hasConceptScore W2958604821C90355303 @default.
• W2958604821 hasIssue "7" @default.
• W2958604821 hasLocation W29586048211 @default.
• W2958604821 hasLocation W29586048212 @default.
• W2958604821 hasLocation W29586048213 @default.
• W2958604821 hasLocation W29586048214 @default.
• W2958604821 hasLocation W29586048215 @default.
• W2958604821 hasLocation W29586048216 @default.
• W2958604821 hasOpenAccess W2958604821 @default.

• next