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• W2959436900 abstract "Abstract Faithful duplication of genomic DNA relies not only on the fidelity of DNA replication itself, but also on fully functional DNA repair and homologous recombination machinery. We report a molecular mechanism responsible for deciding homologous recombinational repair pathways during replication dictated by binding of RecO and RecG to SSB in E.coli. Using a RecG-yfp fusion protein, we found that RecG-yfp foci appeared only in the Δ rec G, Δ rec O and Δ rec A, Δ rec O double mutants. Surprisingly, foci were not observed in wild-type Δ rec G, or double mutants where recG and either recF or, separately recR were deleted. In addition, formation of RecG-yfp foci in the Δ rec O::kan R required wildtype ssb , as ssb-113 could not substitute. This suggests that RecG and RecO binding to SSB is competitive. We also found that the UV resistance of rec O alone mutant increased to certain extent by supplementing RecG. In an ssb-113 mutant, RecO and RecG worked following a different pattern. Both RecO and RecG were able to participate in repairing UV damages when grown at permissive temperature, while they could also be involved in making DNA double strand breaks when grown at nonpermissive temperature. So, our results suggested that differential binding of RecG and RecO to SSB in a DNA replication fork in Escherichia coli .may be involved in determining whether the SDSA or DSBR pathway of homologous recombinational repair is used. Author summary Single strand DNA binding proteins (SSB) stabilize DNA holoenzyme and prevent single strand DNA from folding into non-B DNA structures in a DNA replication fork. It has also been revealed that SSB can also act as a platform for some proteins working in DNA repair and recombination to access DNA molecules when DNA replication fork needs to be reestablished. In Escherichia coli , several proteins working primarily in DNA repair and recombination were found to participate in DNA replication fork resumption by physically interacting with SSB, including RecO and RecG etc. However the hierarchy of these proteins interacting with SSB in Escherichia coli has not been well defined. In this study, we demonstrated a differential binding of RecO and RecG to SSB in DNA replication was used to establish a RecO-dependent pathway of replication fork repair by abolishing a RecG-dependent replication fork repair. We also show that, RecG and RecO could randomly participate in DNA replication repair in the absence of a functional SSB, which may be responsible for the generation of DNA double strand breaks in an ssb -113 mutant in Escherichia coli." @default.
• W2959436900 created "2019-07-23" @default.
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• W2959436900 date "2019-07-19" @default.
• W2959436900 modified "2023-09-27" @default.
• W2959436900 title "RecO impedes RecG-SSB binding to impair the strand annealing recombination pathway inE.coli" @default.
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• W2959436900 cites W1570361288 @default.
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• W2959436900 cites W1965780315 @default.
• W2959436900 cites W1966260649 @default.
• W2959436900 cites W1968035605 @default.
• W2959436900 cites W1968288171 @default.
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• W2959436900 cites W1975853779 @default.
• W2959436900 cites W1981844387 @default.
• W2959436900 cites W1985112184 @default.
• W2959436900 cites W1989108993 @default.
• W2959436900 cites W1989798735 @default.
• W2959436900 cites W1992140093 @default.
• W2959436900 cites W2001556933 @default.
• W2959436900 cites W2002333699 @default.
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• W2959436900 cites W2011351273 @default.
• W2959436900 cites W2025223303 @default.
• W2959436900 cites W2025821843 @default.
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• W2959436900 cites W2028622989 @default.
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• W2959436900 cites W2039764581 @default.
• W2959436900 cites W2048100858 @default.
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• W2959436900 cites W2048727149 @default.
• W2959436900 cites W2052703427 @default.
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• W2959436900 cites W2080857411 @default.
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• W2959436900 cites W2084158547 @default.
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• W2959436900 cites W2091366963 @default.
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• W2959436900 cites W2131737890 @default.
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• W2959436900 doi "https://doi.org/10.1101/708271" @default.
• W2959436900 hasPublicationYear "2019" @default.
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