FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2959590321> ?p ?o ?g. }

• W2959590321 abstract "Abstract Xylella fastidiosa is an insect-borne bacterium confined to the xylem vessels of plants. This plant pathogen has a broad host range estimated to 560 plant species. Five subspecies of the pathogen with different but overlapping host ranges have been described, but only three subspecies are widely accepted, namely subspecies fastidiosa , multiplex and pauca . Initially limited to the Americas, Xf has been detected in Europe since 2013. As management of X. fastidiosa outbreaks in Europe depends on the identification of the subspecies, accurate determination of the subspecies in infected plants as early as possible is of major interest. Thus, we developed various tetraplex and triplex qPCR assays for Xylella fastidiosa detection and subspecies identification in planta in a single reaction. We designed primers and probes using SkIf, a bioinformatics tool based on k -mers, to detect specific signatures of the species and subspecies from a dataset of 58 genome sequences representative of X. fastidiosa diversity. We tested the qPCR assays on 39 target and 30 non-target strains, as well as on 13 different plant species spiked with strains of the different subspecies of X. fastidiosa , and on samples from various environmental and inoculated host plants. Sensitivity of simplex assays was equal or slightly better than the reference protocol on purified DNA. Tetraplex qPCR assays had the same sensitivity than the reference protocol and allowed X. fastidiosa detection in all spiked matrices up to 10 3 cells.mL −1 . Moreover, mix infections of two to three subspecies could be detected in the same sample with tetraplex assays. In environmental plant samples, the tetraplex qPCR assays allowed subspecies identification when the current method based on multilocus sequence typing failed. The qPCR assays described here are robust and modular tools that are efficient for differentiating X. fastidiosa subspecies directly in plant samples." @default.
• W2959590321 created "2019-07-23" @default.
• W2959590321 creator A5023855337 @default.
• W2959590321 creator A5031148000 @default.
• W2959590321 creator A5079298662 @default.
• W2959590321 creator A5083647639 @default.
• W2959590321 date "2019-07-11" @default.
• W2959590321 modified "2023-10-01" @default.
• W2959590321 title "Novel tetraplex qPCR assays for simultaneous detection and identification ofXylella fastidiosasubspecies in plant tissues" @default.
• W2959590321 cites W1551613500 @default.
• W2959590321 cites W1574956921 @default.
• W2959590321 cites W1940068687 @default.
• W2959590321 cites W1973207608 @default.
• W2959590321 cites W1977516923 @default.
• W2959590321 cites W1977787738 @default.
• W2959590321 cites W1989811043 @default.
• W2959590321 cites W2010146342 @default.
• W2959590321 cites W2022991228 @default.
• W2959590321 cites W2023003008 @default.
• W2959590321 cites W2023835250 @default.
• W2959590321 cites W2024041324 @default.
• W2959590321 cites W2027513544 @default.
• W2959590321 cites W2032121867 @default.
• W2959590321 cites W2037648830 @default.
• W2959590321 cites W2053868535 @default.
• W2959590321 cites W2056091989 @default.
• W2959590321 cites W2083564350 @default.
• W2959590321 cites W2083675580 @default.
• W2959590321 cites W2097723733 @default.
• W2959590321 cites W2100303227 @default.
• W2959590321 cites W2104078322 @default.
• W2959590321 cites W2113140373 @default.
• W2959590321 cites W2138183126 @default.
• W2959590321 cites W2143808263 @default.
• W2959590321 cites W2155313208 @default.
• W2959590321 cites W2157333322 @default.
• W2959590321 cites W2168420558 @default.
• W2959590321 cites W2179631201 @default.
• W2959590321 cites W2264351142 @default.
• W2959590321 cites W2274392210 @default.
• W2959590321 cites W2408458705 @default.
• W2959590321 cites W2558605286 @default.
• W2959590321 cites W2589209312 @default.
• W2959590321 cites W2590101155 @default.
• W2959590321 cites W2727108621 @default.
• W2959590321 cites W2748568791 @default.
• W2959590321 cites W2757920376 @default.
• W2959590321 cites W2788714424 @default.
• W2959590321 cites W2792086724 @default.
• W2959590321 cites W2807925761 @default.
• W2959590321 cites W2810878102 @default.
• W2959590321 cites W2891656050 @default.
• W2959590321 cites W2893949388 @default.
• W2959590321 cites W2900074677 @default.
• W2959590321 cites W2900510202 @default.
• W2959590321 cites W2900592415 @default.
• W2959590321 cites W2908608394 @default.
• W2959590321 cites W2910501984 @default.
• W2959590321 cites W2912296822 @default.
• W2959590321 cites W2923901320 @default.
• W2959590321 cites W2945705509 @default.
• W2959590321 cites W2951755970 @default.
• W2959590321 cites W4211212399 @default.
• W2959590321 cites W4242407984 @default.
• W2959590321 cites W4251909043 @default.
• W2959590321 doi "https://doi.org/10.1101/699371" @default.
• W2959590321 hasPublicationYear "2019" @default.
• W2959590321 type Work @default.
• W2959590321 sameAs 2959590321 @default.
• W2959590321 citedByCount "4" @default.
• W2959590321 countsByYear W29595903212019 @default.
• W2959590321 countsByYear W29595903212020 @default.
• W2959590321 countsByYear W29595903212021 @default.
• W2959590321 crossrefType "posted-content" @default.
• W2959590321 hasAuthorship W2959590321A5023855337 @default.
• W2959590321 hasAuthorship W2959590321A5031148000 @default.
• W2959590321 hasAuthorship W2959590321A5079298662 @default.
• W2959590321 hasAuthorship W2959590321A5083647639 @default.
• W2959590321 hasBestOaLocation W29595903211 @default.
• W2959590321 hasConcept C116834253 @default.
• W2959590321 hasConcept C126831891 @default.
• W2959590321 hasConcept C17966761 @default.
• W2959590321 hasConcept C2776460866 @default.
• W2959590321 hasConcept C2778410202 @default.
• W2959590321 hasConcept C2781188995 @default.
• W2959590321 hasConcept C523546767 @default.
• W2959590321 hasConcept C54355233 @default.
• W2959590321 hasConcept C59822182 @default.
• W2959590321 hasConcept C86803240 @default.
• W2959590321 hasConcept C89423630 @default.
• W2959590321 hasConcept C90856448 @default.
• W2959590321 hasConceptScore W2959590321C116834253 @default.
• W2959590321 hasConceptScore W2959590321C126831891 @default.
• W2959590321 hasConceptScore W2959590321C17966761 @default.
• W2959590321 hasConceptScore W2959590321C2776460866 @default.
• W2959590321 hasConceptScore W2959590321C2778410202 @default.
• W2959590321 hasConceptScore W2959590321C2781188995 @default.
• W2959590321 hasConceptScore W2959590321C523546767 @default.
• W2959590321 hasConceptScore W2959590321C54355233 @default.
• W2959590321 hasConceptScore W2959590321C59822182 @default.

• next