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• W2959591402 abstract "We developed a fully-automated dicentric chromosome assay (DCA) in multiwell plates. All operations, from sample loading to chromosome scoring, are performed, without human intervention, by the second-generation Rapid Automated Biodosimetry Tool II (RABiT-II) robotic system, a plate imager and custom software, FluorQuantDic. The system requires small volumes of blood (30 µl per individual) to determine radiation dose received as a result of a radiation accident or terrorist attack. To visualize dicentrics in multiwell plates, we implemented a non-classical protocol for centromere FISH staining at 37°C. The RABiT-II performs rapid analysis of chromosomes after extracting them from metaphase cells. With the use of multiwell plates, many samples can be screened at the same time. Thus, the RABiT-II DCA provides an advantage during triage when risk-based stratification and medical management are required for a large population exposed to unknown levels of ionizing radiation." @default.
• W2959591402 created "2019-07-23" @default.
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• W2959591402 date "2019-07-11" @default.
• W2959591402 modified "2023-10-01" @default.
• W2959591402 title "RABiT-II-DCA: A Fully-automated Dicentric Chromosome Assay in Multiwell Plates" @default.
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• W2959591402 doi "https://doi.org/10.1667/rr15266.1" @default.
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