Matches in SemOpenAlex for { <https://semopenalex.org/work/W2959656963> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W2959656963 endingPage "734305" @default.
- W2959656963 startingPage "734305" @default.
- W2959656963 abstract "Abstract The release or escape of genetically modified fish from confined aquaculture facilities presents a potential risk to wild fish stocks and surrounding ecosystems. Thus, reliable biocontainment methods for aquaculture-relevant species are needed. Since physical barriers are not 100% effective, it is critical to develop reliable genetic-based methods for biocontainment. RNA interference (RNAi) could regulate gene expression and target genes that are essential for fish viability and/or reproduction. One such target gene, deadend (dnd), is required for primordial germ cell (PGC) development, migration and survival. While the use of RNAi technology has been successfully reported in C. elegans, M. musculus, and D. melanogaster, its use in zebrafish (Danio rerio) has been met with variable success. We targeted dnd using two different short-hairpin RNA (shRNA) approaches in zebrafish embryos, namely, 1) CMV-driven or a Gal4/UAS constructs with one double short-hairpin RNA stem-loop design consisting of two different target sites, and 2) CMV-driven to test two separate target sites independently flanked by the 5′ and 3′ sequences of the pri-miR-30e miRNA. The first shRNA strategy did not completely nor consistently abolish PGCs, based on vasa-expression, but low vasa expression was observed in some fish at 6 dpf. In contrast, the second shRNA strategy resulted in reduced and/or ectopic positioning of PGCs based on vasa expression at 2 and 6 dpf. However, neither strategy affected fertility in adult fish." @default.
- W2959656963 created "2019-07-23" @default.
- W2959656963 creator A5026189603 @default.
- W2959656963 creator A5032876459 @default.
- W2959656963 creator A5034675569 @default.
- W2959656963 creator A5058422538 @default.
- W2959656963 creator A5060722786 @default.
- W2959656963 date "2019-10-01" @default.
- W2959656963 modified "2023-10-17" @default.
- W2959656963 title "Dnd shRNA constructs cause altered PGC migration but fail to sterilize" @default.
- W2959656963 cites W144423133 @default.
- W2959656963 cites W1647075334 @default.
- W2959656963 cites W1967633197 @default.
- W2959656963 cites W1972937465 @default.
- W2959656963 cites W1979667801 @default.
- W2959656963 cites W1983538883 @default.
- W2959656963 cites W1993875474 @default.
- W2959656963 cites W1993883459 @default.
- W2959656963 cites W1996163490 @default.
- W2959656963 cites W1996689072 @default.
- W2959656963 cites W2009375691 @default.
- W2959656963 cites W2010747656 @default.
- W2959656963 cites W2017885336 @default.
- W2959656963 cites W2024719302 @default.
- W2959656963 cites W2048690485 @default.
- W2959656963 cites W2051401308 @default.
- W2959656963 cites W2053188090 @default.
- W2959656963 cites W2059918619 @default.
- W2959656963 cites W2070133209 @default.
- W2959656963 cites W2074911766 @default.
- W2959656963 cites W2083035724 @default.
- W2959656963 cites W2133898735 @default.
- W2959656963 cites W2137168087 @default.
- W2959656963 cites W2158423656 @default.
- W2959656963 cites W2161074631 @default.
- W2959656963 cites W2166542953 @default.
- W2959656963 cites W2175040064 @default.
- W2959656963 cites W2294339661 @default.
- W2959656963 cites W2342004244 @default.
- W2959656963 cites W2406910575 @default.
- W2959656963 cites W2467019116 @default.
- W2959656963 cites W2571251495 @default.
- W2959656963 cites W2901863971 @default.
- W2959656963 cites W4251375829 @default.
- W2959656963 doi "https://doi.org/10.1016/j.aquaculture.2019.734305" @default.
- W2959656963 hasPublicationYear "2019" @default.
- W2959656963 type Work @default.
- W2959656963 sameAs 2959656963 @default.
- W2959656963 citedByCount "0" @default.
- W2959656963 crossrefType "journal-article" @default.
- W2959656963 hasAuthorship W2959656963A5026189603 @default.
- W2959656963 hasAuthorship W2959656963A5032876459 @default.
- W2959656963 hasAuthorship W2959656963A5034675569 @default.
- W2959656963 hasAuthorship W2959656963A5058422538 @default.
- W2959656963 hasAuthorship W2959656963A5060722786 @default.
- W2959656963 hasConcept C104317684 @default.
- W2959656963 hasConcept C145059251 @default.
- W2959656963 hasConcept C166703698 @default.
- W2959656963 hasConcept C190232843 @default.
- W2959656963 hasConcept C196843134 @default.
- W2959656963 hasConcept C2776878037 @default.
- W2959656963 hasConcept C2780384893 @default.
- W2959656963 hasConcept C54355233 @default.
- W2959656963 hasConcept C67705224 @default.
- W2959656963 hasConcept C70721500 @default.
- W2959656963 hasConcept C86803240 @default.
- W2959656963 hasConcept C95444343 @default.
- W2959656963 hasConceptScore W2959656963C104317684 @default.
- W2959656963 hasConceptScore W2959656963C145059251 @default.
- W2959656963 hasConceptScore W2959656963C166703698 @default.
- W2959656963 hasConceptScore W2959656963C190232843 @default.
- W2959656963 hasConceptScore W2959656963C196843134 @default.
- W2959656963 hasConceptScore W2959656963C2776878037 @default.
- W2959656963 hasConceptScore W2959656963C2780384893 @default.
- W2959656963 hasConceptScore W2959656963C54355233 @default.
- W2959656963 hasConceptScore W2959656963C67705224 @default.
- W2959656963 hasConceptScore W2959656963C70721500 @default.
- W2959656963 hasConceptScore W2959656963C86803240 @default.
- W2959656963 hasConceptScore W2959656963C95444343 @default.
- W2959656963 hasFunder F4320334593 @default.
- W2959656963 hasLocation W29596569631 @default.
- W2959656963 hasOpenAccess W2959656963 @default.
- W2959656963 hasPrimaryLocation W29596569631 @default.
- W2959656963 hasRelatedWork W11010260 @default.
- W2959656963 hasRelatedWork W12615696 @default.
- W2959656963 hasRelatedWork W14272306 @default.
- W2959656963 hasRelatedWork W2294226 @default.
- W2959656963 hasRelatedWork W25589616 @default.
- W2959656963 hasRelatedWork W27552248 @default.
- W2959656963 hasRelatedWork W32907388 @default.
- W2959656963 hasRelatedWork W3954831 @default.
- W2959656963 hasRelatedWork W40814692 @default.
- W2959656963 hasRelatedWork W9814085 @default.
- W2959656963 hasVolume "512" @default.
- W2959656963 isParatext "false" @default.
- W2959656963 isRetracted "false" @default.
- W2959656963 magId "2959656963" @default.
- W2959656963 workType "article" @default.