FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2959659611> ?p ?o ?g. }

• W2959659611 abstract "Author(s): Henske, John | Advisor(s): O'Malley, Michelle A | Abstract: The development of a renewable, bio-based economy requires efficient methods to extract fermentable sugars from complex plant material. Currently, bioprocessing from crude biomass requires multiple steps including pretreatment to separate lignin from sugar-rich cellulose and hemicellulose, enzymatic hydrolysis to release simple sugars, and microbial fermentation to produce value-added chemicals. Consolidated bioprocessing seeks to improve bioprocessing efficiency by reducing the number of steps required to get from plant biomass to chemical product. To address this challenge, we derived inspiration from natural microbial communities known for degrading biomass. Within the rumen microbiome of large herbivores, anaerobic gut fungi are the primary colonizers of plant material and present an untapped opportunity for consolidated bioprocessing. These unique microorganisms efficiently hydrolyze lignocellulosic biomass into simple sugars, but remain relatively uncharacterized in comparison to industrial production organisms. We implemented Next Generation Sequencing (NGS) technologies alongside biochemical studies to develop a deeper understanding of gut fungi, their metabolism, and the mechanisms by which they break down complex biomass to identify a path forward for their industrial application. We also developed simple, rapid methodologies for cryopreservation and DNA extraction that are critical for the development of industrial microbes.Sequencing and functional annotation of transcriptomes and genomes of novel isolated species of gut fungi has elucidated their large repertoire of biomass degrading enzymes including cellulases, hemicellulases, and accessory enzymes. These enzymes allow them to efficiently degrade crude biomass, yielding similar growth rates on complex plant material and simple sugars. Remarkably, in isolated batch culture, the biomass degrading power of gut fungi is sufficient to generate surplus fermentable sugars for the growth of additional microorganisms. This ability has been exploited to develop a novel two-stage consolidated bioprocessing scheme that uses anaerobic gut fungi to consolidate the pretreatment and hydrolysis steps in traditional bioprocessing to hydrolyze sugars directly from crude biomass. These sugars can then be fed to the easily metabolically engineered model yeast, Saccharomyces cerevisiae, to support growth and bioproduction in a two-stage fermentation scheme. Further, RNA sequencing studies have provided critical insight into the regulation of biomass degrading activity. Gene expression during growth on varying substrates and in response to a carbon catabolite repressor has revealed conditions required to optimize expression of biomass degrading enzymes. Unannotated sequences that co-regulate with predicted biomass degrading enzymes have also been identified as candidate genes that may host novel biomass degrading function. Together these results reveal important process considerations for the use of gut fungi in industrial bioprocessing to maximize the production of enzymes and the degradation of biomass. While challenges remain for the implementation of gut fungi in industrial bioprocessing, we have demonstrated their potential to consolidate pretreatment and hydrolysis either through engineered culturing schemes or development of improved enzyme cocktails for biomass hydrolysis." @default.
• W2959659611 created "2019-07-23" @default.
• W2959659611 creator A5008136817 @default.
• W2959659611 date "2017-01-01" @default.
• W2959659611 modified "2023-09-27" @default.
• W2959659611 title "Engineering Regulation in Anaerobic Gut Fungi during Lignocellulose Breakdown" @default.
• W2959659611 hasPublicationYear "2017" @default.
• W2959659611 type Work @default.
• W2959659611 sameAs 2959659611 @default.
• W2959659611 citedByCount "0" @default.
• W2959659611 crossrefType "journal-article" @default.
• W2959659611 hasAuthorship W2959659611A5008136817 @default.
• W2959659611 hasConcept C100544194 @default.
• W2959659611 hasConcept C115540264 @default.
• W2959659611 hasConcept C141603559 @default.
• W2959659611 hasConcept C150903083 @default.
• W2959659611 hasConcept C151730666 @default.
• W2959659611 hasConcept C181199279 @default.
• W2959659611 hasConcept C2776073808 @default.
• W2959659611 hasConcept C2778234585 @default.
• W2959659611 hasConcept C2779251873 @default.
• W2959659611 hasConcept C2781052789 @default.
• W2959659611 hasConcept C31903555 @default.
• W2959659611 hasConcept C4916135 @default.
• W2959659611 hasConcept C53991642 @default.
• W2959659611 hasConcept C55493867 @default.
• W2959659611 hasConcept C59822182 @default.
• W2959659611 hasConcept C6350086 @default.
• W2959659611 hasConcept C6557445 @default.
• W2959659611 hasConcept C86803240 @default.
• W2959659611 hasConceptScore W2959659611C100544194 @default.
• W2959659611 hasConceptScore W2959659611C115540264 @default.
• W2959659611 hasConceptScore W2959659611C141603559 @default.
• W2959659611 hasConceptScore W2959659611C150903083 @default.
• W2959659611 hasConceptScore W2959659611C151730666 @default.
• W2959659611 hasConceptScore W2959659611C181199279 @default.
• W2959659611 hasConceptScore W2959659611C2776073808 @default.
• W2959659611 hasConceptScore W2959659611C2778234585 @default.
• W2959659611 hasConceptScore W2959659611C2779251873 @default.
• W2959659611 hasConceptScore W2959659611C2781052789 @default.
• W2959659611 hasConceptScore W2959659611C31903555 @default.
• W2959659611 hasConceptScore W2959659611C4916135 @default.
• W2959659611 hasConceptScore W2959659611C53991642 @default.
• W2959659611 hasConceptScore W2959659611C55493867 @default.
• W2959659611 hasConceptScore W2959659611C59822182 @default.
• W2959659611 hasConceptScore W2959659611C6350086 @default.
• W2959659611 hasConceptScore W2959659611C6557445 @default.
• W2959659611 hasConceptScore W2959659611C86803240 @default.
• W2959659611 hasLocation W29596596111 @default.
• W2959659611 hasOpenAccess W2959659611 @default.
• W2959659611 hasPrimaryLocation W29596596111 @default.
• W2959659611 hasRelatedWork W1849814872 @default.
• W2959659611 hasRelatedWork W2015486060 @default.
• W2959659611 hasRelatedWork W2029391779 @default.
• W2959659611 hasRelatedWork W2057249233 @default.
• W2959659611 hasRelatedWork W2121255724 @default.
• W2959659611 hasRelatedWork W2138973274 @default.
• W2959659611 hasRelatedWork W2396014121 @default.
• W2959659611 hasRelatedWork W2750975857 @default.
• W2959659611 hasRelatedWork W2765884377 @default.
• W2959659611 hasRelatedWork W2772885830 @default.
• W2959659611 hasRelatedWork W2782549172 @default.
• W2959659611 hasRelatedWork W2905246724 @default.
• W2959659611 hasRelatedWork W2911989704 @default.
• W2959659611 hasRelatedWork W2925019185 @default.
• W2959659611 hasRelatedWork W2951346340 @default.
• W2959659611 hasRelatedWork W2980949580 @default.
• W2959659611 hasRelatedWork W3155272733 @default.
• W2959659611 hasRelatedWork W3211287828 @default.
• W2959659611 hasRelatedWork W905263996 @default.
• W2959659611 hasRelatedWork W2069526204 @default.
• W2959659611 isParatext "false" @default.
• W2959659611 isRetracted "false" @default.
• W2959659611 magId "2959659611" @default.
• W2959659611 workType "article" @default.