FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2960546467> ?p ?o ?g. }

• W2960546467 endingPage "907" @default.
• W2960546467 startingPage "901" @default.
• W2960546467 abstract "Toxoplasma gondii is an obligate intracellular protozoan parasite that causes toxoplasmosis in humans and animals. Micronemes (MICs) are effective candidates for DNA vaccine.In this study, we evaluated the immune response of BALB/c mice against MIC3 gene of Toxoplasma gondii and interleukin 12 (IL-12) as DNA vaccine. The MIC3 gene was cloned into the PTZ57R/T vector before sub-cloning in pcDNA3. Recombinant pc-MIC3 was transformed into Escherichia coli (TOP10 strain). The pc-MIC3 plasmid was then transfected into Chinese Hamster Ovary (CHO) cells, and the expression of the MIC3 gene was evaluated by SDS-PAGE and Western blotting. Sixty female BALB/c mice were divided into 6 groups. Each group received 3 intramuscular immunizations on days 0, 21st and 42nd using one of the following stimulants: phosphate-buffered saline, pcDNA3, pCAGGS-IL12, pc-MIC3 (100 µg), pc-MIC3 (50 µg), or combined pCAGGS-IL12 (50 µg) and pc-MIC3 (50 µg). The enzyme-linked immunosorbent assays was applied to evaluate interferon gamma (IFN-γ) and IL-4 cytokines excretion of lymphocytes stimulated with tachyzoites lysate antigen, as well as the total levels of immunoglobulin G (IgG), IgG2a and IgG1 in immunized mice sera.Our results showed that mice challenged with pc-MIC3 (100 µg) had the highest longevity and quantity of immunoglobulin. Moreover, the highest expression level of IFN-γ was found in mice injected with combined pcMIC3 and pCAGGS-IL12 (P<0.05).The MIC3 gene can be an efficient DNA vaccine candidate against toxoplasmosis. While, the single-gene vaccine can confer partial protection to mice against toxoplasmosis, the multigene vaccine can significantly enhance immune responses." @default.
• W2960546467 created "2019-07-23" @default.
• W2960546467 creator A5006720918 @default.
• W2960546467 creator A5018318900 @default.
• W2960546467 creator A5022920948 @default.
• W2960546467 creator A5034199022 @default.
• W2960546467 creator A5072366432 @default.
• W2960546467 creator A5072722272 @default.
• W2960546467 date "2019-08-01" @default.
• W2960546467 modified "2023-09-27" @default.
• W2960546467 title "Assessment of DNA vaccine encoding Toxoplasma gondii microneme complete gene and IL-12 as adjuvant in BALB/c mice." @default.
• W2960546467 cites W1963683603 @default.
• W2960546467 cites W1982939092 @default.
• W2960546467 cites W1986909105 @default.
• W2960546467 cites W1996881279 @default.
• W2960546467 cites W2005958919 @default.
• W2960546467 cites W2007835398 @default.
• W2960546467 cites W2014507720 @default.
• W2960546467 cites W2018289835 @default.
• W2960546467 cites W2021282986 @default.
• W2960546467 cites W2034751486 @default.
• W2960546467 cites W2038485506 @default.
• W2960546467 cites W2045214907 @default.
• W2960546467 cites W2045406234 @default.
• W2960546467 cites W2046324669 @default.
• W2960546467 cites W2051961035 @default.
• W2960546467 cites W2054895777 @default.
• W2960546467 cites W2055922898 @default.
• W2960546467 cites W2075717865 @default.
• W2960546467 cites W2097090163 @default.
• W2960546467 cites W2100837269 @default.
• W2960546467 cites W2128635872 @default.
• W2960546467 cites W2138614218 @default.
• W2960546467 cites W2150317523 @default.
• W2960546467 cites W2150841475 @default.
• W2960546467 cites W2162071653 @default.
• W2960546467 cites W2168380071 @default.
• W2960546467 cites W2216120288 @default.
• W2960546467 cites W2465850909 @default.
• W2960546467 cites W2807749543 @default.
• W2960546467 cites W2885647643 @default.
• W2960546467 cites W2912835684 @default.
• W2960546467 cites W3191708682 @default.
• W2960546467 doi "https://doi.org/10.22038/ijbms.2019.34872.8276" @default.
• W2960546467 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6760480" @default.
• W2960546467 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31579446" @default.
• W2960546467 hasPublicationYear "2019" @default.
• W2960546467 type Work @default.
• W2960546467 sameAs 2960546467 @default.
• W2960546467 citedByCount "5" @default.
• W2960546467 countsByYear W29605464672020 @default.
• W2960546467 countsByYear W29605464672021 @default.
• W2960546467 countsByYear W29605464672022 @default.
• W2960546467 crossrefType "journal-article" @default.
• W2960546467 hasAuthorship W2960546467A5006720918 @default.
• W2960546467 hasAuthorship W2960546467A5018318900 @default.
• W2960546467 hasAuthorship W2960546467A5022920948 @default.
• W2960546467 hasAuthorship W2960546467A5034199022 @default.
• W2960546467 hasAuthorship W2960546467A5072366432 @default.
• W2960546467 hasAuthorship W2960546467A5072722272 @default.
• W2960546467 hasConcept C104317684 @default.
• W2960546467 hasConcept C147483822 @default.
• W2960546467 hasConcept C149532602 @default.
• W2960546467 hasConcept C154317977 @default.
• W2960546467 hasConcept C159047783 @default.
• W2960546467 hasConcept C159654299 @default.
• W2960546467 hasConcept C167508917 @default.
• W2960546467 hasConcept C202751555 @default.
• W2960546467 hasConcept C203014093 @default.
• W2960546467 hasConcept C22744801 @default.
• W2960546467 hasConcept C2777863537 @default.
• W2960546467 hasConcept C2778048844 @default.
• W2960546467 hasConcept C2778062946 @default.
• W2960546467 hasConcept C2778128430 @default.
• W2960546467 hasConcept C2778371730 @default.
• W2960546467 hasConcept C2779934537 @default.
• W2960546467 hasConcept C2781023229 @default.
• W2960546467 hasConcept C40767141 @default.
• W2960546467 hasConcept C55493867 @default.
• W2960546467 hasConcept C86803240 @default.
• W2960546467 hasConcept C8891405 @default.
• W2960546467 hasConcept C89423630 @default.
• W2960546467 hasConceptScore W2960546467C104317684 @default.
• W2960546467 hasConceptScore W2960546467C147483822 @default.
• W2960546467 hasConceptScore W2960546467C149532602 @default.
• W2960546467 hasConceptScore W2960546467C154317977 @default.
• W2960546467 hasConceptScore W2960546467C159047783 @default.
• W2960546467 hasConceptScore W2960546467C159654299 @default.
• W2960546467 hasConceptScore W2960546467C167508917 @default.
• W2960546467 hasConceptScore W2960546467C202751555 @default.
• W2960546467 hasConceptScore W2960546467C203014093 @default.
• W2960546467 hasConceptScore W2960546467C22744801 @default.
• W2960546467 hasConceptScore W2960546467C2777863537 @default.
• W2960546467 hasConceptScore W2960546467C2778048844 @default.
• W2960546467 hasConceptScore W2960546467C2778062946 @default.
• W2960546467 hasConceptScore W2960546467C2778128430 @default.
• W2960546467 hasConceptScore W2960546467C2778371730 @default.
• W2960546467 hasConceptScore W2960546467C2779934537 @default.

• next