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• W2960600003 abstract "microRNA (miR/miRNA)‑27a‑3p has been reported to be abnormally expressed in various types of cancer, including colorectal cancer (CRC). B‑cell translocation gene 1 (BTG1) has also been implicated with CRC. However, the association between miR‑27a‑3p and BTG1 in CRC, to the best of our knowledge, has not been investigated. In order to assess whether miR‑27a‑3p is associated with CRC, reverse transcription‑quantitative PCR was performed on 20 paired CRC and paracancerous tissues for miRNA analysis. For the screening and validation of miR‑27a‑3p expression in colon cancer, several colon cancer cell lines (HCT‑116, HCT8, SW480, HT29, LOVO and Caco2) and the normal colorectal epithelial cell line NCM460 were examined. The highest expression levels of miR‑27a‑3p were detected in the HCT‑116, which was selected for further experimentation. The HCT‑116 cells were divided into control, miR‑27a‑3p mimic and inhibitor groups, and cell proliferation was tested using an MTT assay. Additionally, miR‑27a‑3p inhibitor/mimic or BTG1 plasmid were transfected into the HCT‑116 cells, and flow cytometry was performed to analyze cell cycle distributions. TUNEL analysis was performed to detect apoptosis. Protein levels of factors in the downstream signaling pathway mediated by miR‑27a‑3p [ERK/mitogen‑activated extracellular signal‑regulated kinase (MEK)] were detected. miR‑27a‑3p was revealed to be overexpressed in human CRC tissues and colon cancer cell lines. Knockdown of miR‑27a‑3p suppressed proliferation of HCT‑116 cells and apoptosis was increased. It further markedly upregulated expression levels of BTG1 and inhibited activation of proteins of the ERK/MEK signaling pathway. In addition, overexpression of BTG1 in HCT‑116 cells triggered G1/S phase cell cycle arrest and increased apoptosis via the ERK/MEK signaling pathway. In conclusion, the present study demonstrated that the effects of miR‑27a‑3p on colon cancer cell proliferation and apoptosis were similar to those of the tumor suppressor gene BTG1. The miR‑27a‑3p/BTG1 axis may have potential implications for diagnostic and therapeutic approaches in CRC." @default.
• W2960600003 created "2019-07-23" @default.
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• W2960600003 creator A5076358353 @default.
• W2960600003 date "2019-07-18" @default.
• W2960600003 modified "2023-10-16" @default.
• W2960600003 title "miR‑27a‑3p regulates proliferation and apoptosis of colon cancer cells by potentially targeting BTG1" @default.
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• W2960600003 doi "https://doi.org/10.3892/ol.2019.10629" @default.
• W2960600003 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6676402" @default.
• W2960600003 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31452761" @default.
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