FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2961573450> ?p ?o ?g. }

• W2961573450 abstract "The increased transmission of multidrug-resistant (MDR) tuberculosis (TB) poses a challenge to tuberculosis prevention and control in Sri Lanka. Isoniazid (INH) is a key element of the first line anti tuberculosis treatment regimen. Resistance to INH may lead to development of MDR TB. Therefore, early detection of INH resistance is important to curb spread of resistance. Due to the limited availability of rapid molecular methods for detection of drug resistance in Sri Lanka, this study was aimed at developing a simple and rapid gold nanoparticle (AuNP) based lateral flow strip for the simultaneous detection of the most common INH resistance mutation (katG S315 T, 78.6%) and Mycobacterium tuberculosis (MTb). Lateral flow strip was designed on an inert plastic backing layer containing a sample pad, nitrocellulose membrane and an absorption pad. Biotin labeled 4 capture probes which separately conjugated with streptavidin were immobilized on the nitrocellulose. The test sample was prepared by multiplex PCR using primers to amplify codon 315 region of the katG gene and MTb specific IS6110 region. The two detection probes complementary to the 5′ end of each amplified fragment was conjugated with gold nanoparticles (20 nm) and coupled with the above amplified PCR products were applied on the sample pad. The hybridization of the amplified target regions to the respective capture probes takes place when the sample moves towards the absorption pad. Positive hybridization is indicated by red colour lines. The three immobilized capture probes on the strip (for the detection of TB, katG wild type and mutation) were 100 and 96.6% specific and 100 and 92.1% sensitive respectively. The AuNP based lateral flow assay was capable of differentiating the specific mutation and the wild type along with MTb identification within 3 h." @default.
• W2961573450 created "2019-07-23" @default.
• W2961573450 creator A5004445676 @default.
• W2961573450 creator A5006767811 @default.
• W2961573450 creator A5015773115 @default.
• W2961573450 creator A5034407130 @default.
• W2961573450 creator A5067744246 @default.
• W2961573450 creator A5081598424 @default.
• W2961573450 date "2019-07-12" @default.
• W2961573450 modified "2023-10-10" @default.
• W2961573450 title "Use of nanotechnology for infectious disease diagnostics: application in drug resistant tuberculosis" @default.
• W2961573450 cites W1820894746 @default.
• W2961573450 cites W1970801243 @default.
• W2961573450 cites W1973508501 @default.
• W2961573450 cites W1993991583 @default.
• W2961573450 cites W2007447166 @default.
• W2961573450 cites W2019129341 @default.
• W2961573450 cites W2037084195 @default.
• W2961573450 cites W2044440155 @default.
• W2961573450 cites W2057317821 @default.
• W2961573450 cites W2073094155 @default.
• W2961573450 cites W2076415129 @default.
• W2961573450 cites W2090098499 @default.
• W2961573450 cites W2099462841 @default.
• W2961573450 cites W2112022845 @default.
• W2961573450 cites W2124225476 @default.
• W2961573450 cites W2146288323 @default.
• W2961573450 cites W2146987614 @default.
• W2961573450 cites W2147110427 @default.
• W2961573450 cites W2150589833 @default.
• W2961573450 cites W2153413526 @default.
• W2961573450 cites W2157620029 @default.
• W2961573450 cites W2171793152 @default.
• W2961573450 cites W2290598774 @default.
• W2961573450 cites W2300703173 @default.
• W2961573450 cites W2788330561 @default.
• W2961573450 cites W2800499740 @default.
• W2961573450 cites W3103240054 @default.
• W2961573450 cites W71947775 @default.
• W2961573450 doi "https://doi.org/10.1186/s12879-019-4259-x" @default.
• W2961573450 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6626415" @default.
• W2961573450 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31299893" @default.
• W2961573450 hasPublicationYear "2019" @default.
• W2961573450 type Work @default.
• W2961573450 sameAs 2961573450 @default.
• W2961573450 citedByCount "6" @default.
• W2961573450 countsByYear W29615734502020 @default.
• W2961573450 countsByYear W29615734502021 @default.
• W2961573450 countsByYear W29615734502022 @default.
• W2961573450 countsByYear W29615734502023 @default.
• W2961573450 crossrefType "journal-article" @default.
• W2961573450 hasAuthorship W2961573450A5004445676 @default.
• W2961573450 hasAuthorship W2961573450A5006767811 @default.
• W2961573450 hasAuthorship W2961573450A5015773115 @default.
• W2961573450 hasAuthorship W2961573450A5034407130 @default.
• W2961573450 hasAuthorship W2961573450A5067744246 @default.
• W2961573450 hasAuthorship W2961573450A5081598424 @default.
• W2961573450 hasBestOaLocation W29615734501 @default.
• W2961573450 hasConcept C104819515 @default.
• W2961573450 hasConcept C114851261 @default.
• W2961573450 hasConcept C133936738 @default.
• W2961573450 hasConcept C142724271 @default.
• W2961573450 hasConcept C155672457 @default.
• W2961573450 hasConcept C159047783 @default.
• W2961573450 hasConcept C171250308 @default.
• W2961573450 hasConcept C185592680 @default.
• W2961573450 hasConcept C192562407 @default.
• W2961573450 hasConcept C2776967927 @default.
• W2961573450 hasConcept C2777975735 @default.
• W2961573450 hasConcept C2778954975 @default.
• W2961573450 hasConcept C2781069245 @default.
• W2961573450 hasConcept C2781188995 @default.
• W2961573450 hasConcept C41625074 @default.
• W2961573450 hasConcept C54355233 @default.
• W2961573450 hasConcept C55493867 @default.
• W2961573450 hasConcept C71924100 @default.
• W2961573450 hasConcept C86803240 @default.
• W2961573450 hasConcept C89423630 @default.
• W2961573450 hasConceptScore W2961573450C104819515 @default.
• W2961573450 hasConceptScore W2961573450C114851261 @default.
• W2961573450 hasConceptScore W2961573450C133936738 @default.
• W2961573450 hasConceptScore W2961573450C142724271 @default.
• W2961573450 hasConceptScore W2961573450C155672457 @default.
• W2961573450 hasConceptScore W2961573450C159047783 @default.
• W2961573450 hasConceptScore W2961573450C171250308 @default.
• W2961573450 hasConceptScore W2961573450C185592680 @default.
• W2961573450 hasConceptScore W2961573450C192562407 @default.
• W2961573450 hasConceptScore W2961573450C2776967927 @default.
• W2961573450 hasConceptScore W2961573450C2777975735 @default.
• W2961573450 hasConceptScore W2961573450C2778954975 @default.
• W2961573450 hasConceptScore W2961573450C2781069245 @default.
• W2961573450 hasConceptScore W2961573450C2781188995 @default.
• W2961573450 hasConceptScore W2961573450C41625074 @default.
• W2961573450 hasConceptScore W2961573450C54355233 @default.
• W2961573450 hasConceptScore W2961573450C55493867 @default.
• W2961573450 hasConceptScore W2961573450C71924100 @default.
• W2961573450 hasConceptScore W2961573450C86803240 @default.
• W2961573450 hasConceptScore W2961573450C89423630 @default.
• W2961573450 hasFunder F4320310906 @default.
• W2961573450 hasIssue "1" @default.

• next