FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2962716911> ?p ?o ?g. }

• W2962716911 endingPage "657" @default.
• W2962716911 startingPage "648" @default.
• W2962716911 abstract "Nickel (Ni2+) is an essential mineral nutrient that is required in trace quantities, but exposure to excess amounts can be toxic or carcinogenic. Mechanisms underlying Ni2+ imbalance and toxicity are poorly understood because most analytical methods currently used to probe this metal are invasive or also have toxic effects. To address these problems, a genetically encoded FRET-based probe for nickel metal (FProNiM) was constructed for real-time detection of Ni2+ in live cells. FProNiM was constructed by sandwiching the bacterial periplasmic nickel-binding protein NikA between the fluorescent protein variants ECFP and Venus. Ni2+ binding to FProNiM induced a conformational change that could be detected by a change in fluorescence resonance energy transfer (FRET) ratio (acceptor/donor fluorescence). In vitro studies demonstrated that FProNiM is specific for Ni2+, insensitive to changes in pH from 5.0 to 8.0, and has an affinity (Kd) of 21.6 μM, which allows detection of Ni2+ concentrations from 0.1 to 5000 μM. The sensor variant FProNiM-5 was also expressed in Escherichia coli (E. coli), yeast, and mammalian cells. In each cell type, changes in Ni2+ concentrations were detected with subcellular resolution using confocal microscopy." @default.
• W2962716911 created "2019-07-30" @default.
• W2962716911 creator A5033367589 @default.
• W2962716911 creator A5056810255 @default.
• W2962716911 date "2019-10-01" @default.
• W2962716911 modified "2023-10-16" @default.
• W2962716911 title "Real time quantification of intracellular nickel using genetically encoded FRET-based nanosensor" @default.
• W2962716911 cites W1542263802 @default.
• W2962716911 cites W1972784441 @default.
• W2962716911 cites W1981635340 @default.
• W2962716911 cites W1984043202 @default.
• W2962716911 cites W1988949448 @default.
• W2962716911 cites W1995713813 @default.
• W2962716911 cites W2001731920 @default.
• W2962716911 cites W2006304581 @default.
• W2962716911 cites W2013412105 @default.
• W2962716911 cites W2022372170 @default.
• W2962716911 cites W2024505095 @default.
• W2962716911 cites W2039210066 @default.
• W2962716911 cites W2047584624 @default.
• W2962716911 cites W2049515750 @default.
• W2962716911 cites W2061098044 @default.
• W2962716911 cites W2063572527 @default.
• W2962716911 cites W2070276213 @default.
• W2962716911 cites W2070653651 @default.
• W2962716911 cites W2079594619 @default.
• W2962716911 cites W2080378040 @default.
• W2962716911 cites W2093471356 @default.
• W2962716911 cites W2099033590 @default.
• W2962716911 cites W2102557954 @default.
• W2962716911 cites W2109283416 @default.
• W2962716911 cites W2111202632 @default.
• W2962716911 cites W2120187999 @default.
• W2962716911 cites W2126383924 @default.
• W2962716911 cites W2152887151 @default.
• W2962716911 cites W2161252555 @default.
• W2962716911 cites W2163212995 @default.
• W2962716911 cites W2437577115 @default.
• W2962716911 cites W2472122738 @default.
• W2962716911 cites W2518685332 @default.
• W2962716911 cites W2767745551 @default.
• W2962716911 cites W2912970043 @default.
• W2962716911 cites W2923705978 @default.
• W2962716911 cites W897038524 @default.
• W2962716911 doi "https://doi.org/10.1016/j.ijbiomac.2019.07.115" @default.
• W2962716911 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31330208" @default.
• W2962716911 hasPublicationYear "2019" @default.
• W2962716911 type Work @default.
• W2962716911 sameAs 2962716911 @default.
• W2962716911 citedByCount "9" @default.
• W2962716911 countsByYear W29627169112020 @default.
• W2962716911 countsByYear W29627169112021 @default.
• W2962716911 countsByYear W29627169112022 @default.
• W2962716911 countsByYear W29627169112023 @default.
• W2962716911 crossrefType "journal-article" @default.
• W2962716911 hasAuthorship W2962716911A5033367589 @default.
• W2962716911 hasAuthorship W2962716911A5056810255 @default.
• W2962716911 hasConcept C104317684 @default.
• W2962716911 hasConcept C121332964 @default.
• W2962716911 hasConcept C12554922 @default.
• W2962716911 hasConcept C185592680 @default.
• W2962716911 hasConcept C201663137 @default.
• W2962716911 hasConcept C2778067846 @default.
• W2962716911 hasConcept C547475151 @default.
• W2962716911 hasConcept C55493867 @default.
• W2962716911 hasConcept C62520636 @default.
• W2962716911 hasConcept C86803240 @default.
• W2962716911 hasConcept C91881484 @default.
• W2962716911 hasConcept C96305047 @default.
• W2962716911 hasConceptScore W2962716911C104317684 @default.
• W2962716911 hasConceptScore W2962716911C121332964 @default.
• W2962716911 hasConceptScore W2962716911C12554922 @default.
• W2962716911 hasConceptScore W2962716911C185592680 @default.
• W2962716911 hasConceptScore W2962716911C201663137 @default.
• W2962716911 hasConceptScore W2962716911C2778067846 @default.
• W2962716911 hasConceptScore W2962716911C547475151 @default.
• W2962716911 hasConceptScore W2962716911C55493867 @default.
• W2962716911 hasConceptScore W2962716911C62520636 @default.
• W2962716911 hasConceptScore W2962716911C86803240 @default.
• W2962716911 hasConceptScore W2962716911C91881484 @default.
• W2962716911 hasConceptScore W2962716911C96305047 @default.
• W2962716911 hasFunder F4320320767 @default.
• W2962716911 hasFunder F4320326656 @default.
• W2962716911 hasLocation W29627169111 @default.
• W2962716911 hasLocation W29627169112 @default.
• W2962716911 hasOpenAccess W2962716911 @default.
• W2962716911 hasPrimaryLocation W29627169111 @default.
• W2962716911 hasRelatedWork W1492304498 @default.
• W2962716911 hasRelatedWork W1726251904 @default.
• W2962716911 hasRelatedWork W1998152547 @default.
• W2962716911 hasRelatedWork W2077482001 @default.
• W2962716911 hasRelatedWork W2081184355 @default.
• W2962716911 hasRelatedWork W2082335522 @default.
• W2962716911 hasRelatedWork W2129451979 @default.
• W2962716911 hasRelatedWork W2321148845 @default.
• W2962716911 hasRelatedWork W2952218830 @default.
• W2962716911 hasRelatedWork W3157204075 @default.
• W2962716911 hasVolume "138" @default.

• next