Matches in SemOpenAlex for { <https://semopenalex.org/work/W2962804951> ?p ?o ?g. }
- W2962804951 abstract "Abstract Salisphere-derived adult epithelial cells enriched for progenitor cells have been used to improve saliva production of irradiated mouse salivary glands. Importantly, optimization of the cellular composition of salispheres could improve their regenerative capabilities. The Rho Kinase (ROCK) 1 inhibitor, Y27632, has been used to increase the proliferation and reduce apoptosis of progenitor cells grown in vitro. In this study, we investigated whether Y27632 in different cell media contexts could be used to improve expansion of adult epithelial progenitor cells or to affect their differentiation potential. Application of Y27632 in medium used previously to grow salispheres promoted expansion of Kit + cells, while in simple serum-containing medium Y27632 increased the number of cells that expressed the K5 basal progenitor marker. When tested in a 3D organoid assay, Y27632 enhanced the contribution of adult salispheres to salivary organoids expressing the secretory proacinar marker Aquaporin 5 (AQP5) in response to FGF2 dependent mesenchymal signals. Optimization of epithelial-mesenchymal interactions organoids can be used to improve application of adult salivary progenitor cells in regenerative medicine strategies. Highlights Y27632 promotes Kit + salisphere cell proliferation in salisphere media. Y27632 promotes K5 expression in salispheres cultured in serum containing media. Y27632 treated Kit + salispheres form salivary organoids expressing AQP5." @default.
- W2962804951 created "2019-07-30" @default.
- W2962804951 creator A5016692605 @default.
- W2962804951 creator A5069399431 @default.
- W2962804951 creator A5086091273 @default.
- W2962804951 creator A5087618896 @default.
- W2962804951 date "2019-07-23" @default.
- W2962804951 modified "2023-10-18" @default.
- W2962804951 title "ROCK Inhibitor Increases Proacinar Cells in Adult Salivary Gland Organoids" @default.
- W2962804951 cites W1546082791 @default.
- W2962804951 cites W1577451703 @default.
- W2962804951 cites W1974597211 @default.
- W2962804951 cites W1976645089 @default.
- W2962804951 cites W2000246621 @default.
- W2962804951 cites W2003702099 @default.
- W2962804951 cites W2004495205 @default.
- W2962804951 cites W2005274657 @default.
- W2962804951 cites W2008381030 @default.
- W2962804951 cites W2015607265 @default.
- W2962804951 cites W2017081564 @default.
- W2962804951 cites W2022570365 @default.
- W2962804951 cites W2044928246 @default.
- W2962804951 cites W2060903279 @default.
- W2962804951 cites W2065150051 @default.
- W2962804951 cites W2072592204 @default.
- W2962804951 cites W2074093109 @default.
- W2962804951 cites W2089361444 @default.
- W2962804951 cites W2102920593 @default.
- W2962804951 cites W2109223404 @default.
- W2962804951 cites W2109939725 @default.
- W2962804951 cites W2111170486 @default.
- W2962804951 cites W2116100337 @default.
- W2962804951 cites W2121201217 @default.
- W2962804951 cites W2126432370 @default.
- W2962804951 cites W2138253156 @default.
- W2962804951 cites W2144662833 @default.
- W2962804951 cites W2150667223 @default.
- W2962804951 cites W2279282310 @default.
- W2962804951 cites W2468452764 @default.
- W2962804951 cites W2524820142 @default.
- W2962804951 cites W2530044565 @default.
- W2962804951 cites W2543020994 @default.
- W2962804951 cites W2556195001 @default.
- W2962804951 cites W2559467837 @default.
- W2962804951 cites W2568577966 @default.
- W2962804951 cites W2758596610 @default.
- W2962804951 cites W2782816981 @default.
- W2962804951 cites W2791004613 @default.
- W2962804951 cites W2884638590 @default.
- W2962804951 cites W2891683811 @default.
- W2962804951 cites W2892107476 @default.
- W2962804951 cites W2896566779 @default.
- W2962804951 cites W2900023726 @default.
- W2962804951 cites W2911512449 @default.
- W2962804951 cites W778542169 @default.
- W2962804951 doi "https://doi.org/10.1101/712877" @default.
- W2962804951 hasPublicationYear "2019" @default.
- W2962804951 type Work @default.
- W2962804951 sameAs 2962804951 @default.
- W2962804951 citedByCount "0" @default.
- W2962804951 crossrefType "posted-content" @default.
- W2962804951 hasAuthorship W2962804951A5016692605 @default.
- W2962804951 hasAuthorship W2962804951A5069399431 @default.
- W2962804951 hasAuthorship W2962804951A5086091273 @default.
- W2962804951 hasAuthorship W2962804951A5087618896 @default.
- W2962804951 hasBestOaLocation W29628049511 @default.
- W2962804951 hasConcept C15729860 @default.
- W2962804951 hasConcept C185592680 @default.
- W2962804951 hasConcept C198826908 @default.
- W2962804951 hasConcept C201750760 @default.
- W2962804951 hasConcept C28328180 @default.
- W2962804951 hasConcept C31695470 @default.
- W2962804951 hasConcept C54355233 @default.
- W2962804951 hasConcept C81885089 @default.
- W2962804951 hasConcept C86803240 @default.
- W2962804951 hasConcept C95444343 @default.
- W2962804951 hasConceptScore W2962804951C15729860 @default.
- W2962804951 hasConceptScore W2962804951C185592680 @default.
- W2962804951 hasConceptScore W2962804951C198826908 @default.
- W2962804951 hasConceptScore W2962804951C201750760 @default.
- W2962804951 hasConceptScore W2962804951C28328180 @default.
- W2962804951 hasConceptScore W2962804951C31695470 @default.
- W2962804951 hasConceptScore W2962804951C54355233 @default.
- W2962804951 hasConceptScore W2962804951C81885089 @default.
- W2962804951 hasConceptScore W2962804951C86803240 @default.
- W2962804951 hasConceptScore W2962804951C95444343 @default.
- W2962804951 hasLocation W29628049511 @default.
- W2962804951 hasLocation W29628049512 @default.
- W2962804951 hasLocation W29628049513 @default.
- W2962804951 hasOpenAccess W2962804951 @default.
- W2962804951 hasPrimaryLocation W29628049511 @default.
- W2962804951 hasRelatedWork W1563959780 @default.
- W2962804951 hasRelatedWork W1746815941 @default.
- W2962804951 hasRelatedWork W1944651517 @default.
- W2962804951 hasRelatedWork W1986072503 @default.
- W2962804951 hasRelatedWork W2005020704 @default.
- W2962804951 hasRelatedWork W2028516177 @default.
- W2962804951 hasRelatedWork W2069882108 @default.
- W2962804951 hasRelatedWork W2146701605 @default.
- W2962804951 hasRelatedWork W2285994234 @default.