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• W2962824105 abstract "Pseudomonas aeruginosa secretes pyoverdine, a major siderophore to get access to iron, an essential nutrient. Pyoverdine scavenges ferric iron in the bacterial environment with the resulting complex internalized by bacteria. Releasing of iron from pyoverdine in the periplasm involves an iron reduction by an inner membrane reductase and two solute‐binding proteins ( SBP s) FpvC and FpvF in association with their ABC transporter. FpvC and FpvF belong to two different subgroups of SBP s within the structural cluster A: FpvC and FpvF were proposed to be a metal‐binding protein and a ferrisiderophore‐binding protein respectively. Here, we report the redox state and the binding mode of iron to FpvC. We first solved the crystal structure of FpvC bound to a fortuitous Ni 2+ by single anomalous dispersion method. Using a different protein purification strategy, we determined the structure of FpvC with manganese and iron, which binds to FpvC in a ferrous state as demonstrated by electron paramagnetic resonance. FpvC is the first example of a hexahistidine metal site among SBP s in which the Fe 2+ redox state is stabilized under aerobic conditions. Using biophysics methods, we showed that FpvC reversibly bind to a broad range of divalent ions. The structure of a mutant mimicking the apo FpvC reveals a protein in an open state with large conformational changes when compared with the metal‐bound FpvC. These results highlight that the canonical metal site in FpvC is distinct from those yet described in SBP s and they provide new insights into the mechanism of PVD ‐Fe dissociation in P. aeruginosa ." @default.
• W2962824105 created "2019-07-30" @default.
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• W2962824105 date "2019-07-26" @default.
• W2962824105 modified "2023-10-17" @default.
• W2962824105 title "A unique ferrous iron binding mode is associated with large conformational changes for the transport protein FpvC of<i>Pseudomonas aeruginosa</i>" @default.
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• W2962824105 doi "https://doi.org/10.1111/febs.15004" @default.
• W2962824105 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31318478" @default.
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