FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2962893048> ?p ?o ?g. }

• W2962893048 abstract "Widespread implementation of Alzheimer's disease biomarkers in routine clinical practice requires the establishment of standard operating procedures for pre-analytical handling of cerebrospinal fluid (CSF).Here, CSF collection and storage protocols were optimized for measurements of β-amyloid (Aβ). We investigated the effects of (1) storage temperature, (2) storage time, (3) centrifugation, (4) sample mixing, (5) blood contamination, and (6) collection gradient on CSF levels of Aβ. For each study participant, we used fresh CSF directly collected into a protein low binding (LoB) tube that was analyzed within hours after lumbar puncture (LP) as standard of truth. Aβ42 and Aβ40 were measured in de-identified CSF samples using EUROIMMUN and Mesoscale discovery assays.CSF Aβ42 and Aβ40 were stable for at least 72 h at room temperature (RT), 1 week at 4 °C, and 2 weeks at - 20 °C and - 80 °C. Centrifugation of non-blood-contaminated CSF or mixing of samples before the analysis did not affect Aβ levels. Addition of 0.1-10% blood to CSF that was stored at RT without centrifugation led to a dose- and time-dependent decrease in Aβ42 and Aβ40, while Aβ42/Aβ40 did not change. The effects of blood contamination were mitigated by centrifugation and/or storage at 4 °C or - 20 °C. Aβ levels did not differ between the first to fourth 5-ml portions of CSF.CSF can be stored for up to 72 h at RT, 1 week at 4 °C, or at least 2 weeks at either - 20 °C or - 80 °C before Aβ measurements. Centrifugation of fresh non-blood-contaminated CSF after LP, or mixing before analysis, is not required. In case of visible blood contamination, centrifugation and storage at 4 °C or - 20 °C is recommended. After discarding the first 2 ml, any portion of up to 20 ml of CSF is suitable for Aβ analysis. These findings will be important for the development of a clinical routine protocol for pre-analytical handling of CSF." @default.
• W2962893048 created "2019-07-30" @default.
• W2962893048 creator A5024645775 @default.
• W2962893048 creator A5040409706 @default.
• W2962893048 creator A5049277633 @default.
• W2962893048 creator A5083219599 @default.
• W2962893048 date "2019-07-19" @default.
• W2962893048 modified "2023-10-12" @default.
• W2962893048 title "Towards a unified protocol for handling of CSF before β-amyloid measurements" @default.
• W2962893048 cites W1484352303 @default.
• W2962893048 cites W1587790985 @default.
• W2962893048 cites W1829034311 @default.
• W2962893048 cites W1964802304 @default.
• W2962893048 cites W1965098511 @default.
• W2962893048 cites W1983874275 @default.
• W2962893048 cites W1990065406 @default.
• W2962893048 cites W2002387090 @default.
• W2962893048 cites W2018871318 @default.
• W2962893048 cites W2019655958 @default.
• W2962893048 cites W2022163852 @default.
• W2962893048 cites W2030246458 @default.
• W2962893048 cites W2038609125 @default.
• W2962893048 cites W2055863273 @default.
• W2962893048 cites W2063435626 @default.
• W2962893048 cites W2064911442 @default.
• W2962893048 cites W2099354100 @default.
• W2962893048 cites W2106156541 @default.
• W2962893048 cites W2111951761 @default.
• W2962893048 cites W2115017507 @default.
• W2962893048 cites W2116423249 @default.
• W2962893048 cites W2116709840 @default.
• W2962893048 cites W2126928570 @default.
• W2962893048 cites W2137570301 @default.
• W2962893048 cites W2207877462 @default.
• W2962893048 cites W2358189270 @default.
• W2962893048 cites W2397701197 @default.
• W2962893048 cites W2418028676 @default.
• W2962893048 cites W2460076813 @default.
• W2962893048 cites W2505351854 @default.
• W2962893048 cites W2582524520 @default.
• W2962893048 cites W2590478248 @default.
• W2962893048 cites W2593953154 @default.
• W2962893048 cites W2621864910 @default.
• W2962893048 cites W2767595531 @default.
• W2962893048 cites W2789976306 @default.
• W2962893048 cites W2794139162 @default.
• W2962893048 cites W2811128719 @default.
• W2962893048 cites W2896430225 @default.
• W2962893048 cites W815595693 @default.
• W2962893048 doi "https://doi.org/10.1186/s13195-019-0517-9" @default.
• W2962893048 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6642586" @default.
• W2962893048 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31324260" @default.
• W2962893048 hasPublicationYear "2019" @default.
• W2962893048 type Work @default.
• W2962893048 sameAs 2962893048 @default.
• W2962893048 citedByCount "36" @default.
• W2962893048 countsByYear W29628930482019 @default.
• W2962893048 countsByYear W29628930482020 @default.
• W2962893048 countsByYear W29628930482021 @default.
• W2962893048 countsByYear W29628930482022 @default.
• W2962893048 countsByYear W29628930482023 @default.
• W2962893048 crossrefType "journal-article" @default.
• W2962893048 hasAuthorship W2962893048A5024645775 @default.
• W2962893048 hasAuthorship W2962893048A5040409706 @default.
• W2962893048 hasAuthorship W2962893048A5049277633 @default.
• W2962893048 hasAuthorship W2962893048A5083219599 @default.
• W2962893048 hasBestOaLocation W29628930481 @default.
• W2962893048 hasConcept C112570922 @default.
• W2962893048 hasConcept C142724271 @default.
• W2962893048 hasConcept C185592680 @default.
• W2962893048 hasConcept C18903297 @default.
• W2962893048 hasConcept C194828623 @default.
• W2962893048 hasConcept C2779651940 @default.
• W2962893048 hasConcept C2780066322 @default.
• W2962893048 hasConcept C2994091943 @default.
• W2962893048 hasConcept C43617362 @default.
• W2962893048 hasConcept C71924100 @default.
• W2962893048 hasConcept C7623868 @default.
• W2962893048 hasConcept C86803240 @default.
• W2962893048 hasConceptScore W2962893048C112570922 @default.
• W2962893048 hasConceptScore W2962893048C142724271 @default.
• W2962893048 hasConceptScore W2962893048C185592680 @default.
• W2962893048 hasConceptScore W2962893048C18903297 @default.
• W2962893048 hasConceptScore W2962893048C194828623 @default.
• W2962893048 hasConceptScore W2962893048C2779651940 @default.
• W2962893048 hasConceptScore W2962893048C2780066322 @default.
• W2962893048 hasConceptScore W2962893048C2994091943 @default.
• W2962893048 hasConceptScore W2962893048C43617362 @default.
• W2962893048 hasConceptScore W2962893048C71924100 @default.
• W2962893048 hasConceptScore W2962893048C7623868 @default.
• W2962893048 hasConceptScore W2962893048C86803240 @default.
• W2962893048 hasFunder F4320322168 @default.
• W2962893048 hasFunder F4320322327 @default.
• W2962893048 hasFunder F4320322581 @default.
• W2962893048 hasFunder F4320327344 @default.
• W2962893048 hasIssue "1" @default.
• W2962893048 hasLocation W29628930481 @default.
• W2962893048 hasLocation W29628930482 @default.
• W2962893048 hasLocation W29628930483 @default.
• W2962893048 hasLocation W29628930484 @default.

• next