Matches in SemOpenAlex for { <https://semopenalex.org/work/W2963323758> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W2963323758 endingPage "973" @default.
- W2963323758 startingPage "961" @default.
- W2963323758 abstract "Transferrin is an iron binding glycoprotein actively involved in the growth and maintenance of cell cycle. The transferrin receptors expression is increased on growing cancer/tumor cells for absorption of iron through transferrin and participation in biological activity. In this study, a novel method for the purification of transferrin by using magnetic nanoparticles (MNP) is developed and compared with reported method. Magnetic nanoparticles were synthesized by co-precipitation method under hydrothermal conditions in the presence of ammonium hydroxide. MNP were characterized by FTIR, VSM, DLS, TEM, and SEM. Purified transferrin was characterized by SDS-PAGE, MALDI-TOF, ELISA, Western blot, and its activity was further confirmed by iron binding assay and receptor binding assays. Purified transferrin was also conjugated with cysteine capped gold nanoparticles (GNP) and characterized by UV-Vis spectra, TEM, DLS, and fluorescent spectrophotometry. Transferrin conjugated cysteine capped GNP used as a targeted fluorescent probe on gastric cancer, tumor tissue and MDA-MB 231 cancer cells to confirm transferrin receptor binding activity and application as diagnostic probe. The purified transferrin showed stability and activity up to 36 months. The results indicated that the synthesized superamagnetic MNP are good for the purification of transferrin. A good yield of transferrin was purified by this method, good quality and showed active biological activity. GNP conjugated transferrin has a potential to be used in cancer diagnosis as targeted diagnosis probe in vivo and in vitro. Experiments are underway for utilizing transferrin as carrier for targeting drug delivery." @default.
- W2963323758 created "2019-07-30" @default.
- W2963323758 creator A5067953714 @default.
- W2963323758 creator A5073044106 @default.
- W2963323758 date "2019-07-18" @default.
- W2963323758 modified "2023-09-25" @default.
- W2963323758 title "Purification of transferrin by magnetic nanoparticles and conjugation with cysteine capped gold nanoparticles for targeting diagnostic probes" @default.
- W2963323758 cites W114062130 @default.
- W2963323758 cites W1527704924 @default.
- W2963323758 cites W1588125159 @default.
- W2963323758 cites W1965451568 @default.
- W2963323758 cites W1968884841 @default.
- W2963323758 cites W1980060032 @default.
- W2963323758 cites W1988318590 @default.
- W2963323758 cites W1992431969 @default.
- W2963323758 cites W1993880194 @default.
- W2963323758 cites W1997784153 @default.
- W2963323758 cites W2008564265 @default.
- W2963323758 cites W2014352467 @default.
- W2963323758 cites W2016915374 @default.
- W2963323758 cites W2027437657 @default.
- W2963323758 cites W2038646538 @default.
- W2963323758 cites W2041290593 @default.
- W2963323758 cites W2044770099 @default.
- W2963323758 cites W2070262613 @default.
- W2963323758 cites W2076614074 @default.
- W2963323758 cites W2077605558 @default.
- W2963323758 cites W2083869690 @default.
- W2963323758 cites W2084871302 @default.
- W2963323758 cites W2085105678 @default.
- W2963323758 cites W2101108802 @default.
- W2963323758 cites W2108545936 @default.
- W2963323758 cites W2117997453 @default.
- W2963323758 cites W2130481949 @default.
- W2963323758 cites W2140497367 @default.
- W2963323758 cites W2151091220 @default.
- W2963323758 cites W2154109173 @default.
- W2963323758 cites W2157826611 @default.
- W2963323758 cites W2527440008 @default.
- W2963323758 cites W2786945793 @default.
- W2963323758 cites W2901987670 @default.
- W2963323758 cites W4293247451 @default.
- W2963323758 cites W2027847278 @default.
- W2963323758 doi "https://doi.org/10.1080/10826068.2019.1643736" @default.
- W2963323758 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31318328" @default.
- W2963323758 hasPublicationYear "2019" @default.
- W2963323758 type Work @default.
- W2963323758 sameAs 2963323758 @default.
- W2963323758 citedByCount "7" @default.
- W2963323758 countsByYear W29633237582020 @default.
- W2963323758 countsByYear W29633237582021 @default.
- W2963323758 countsByYear W29633237582022 @default.
- W2963323758 crossrefType "journal-article" @default.
- W2963323758 hasAuthorship W2963323758A5067953714 @default.
- W2963323758 hasAuthorship W2963323758A5073044106 @default.
- W2963323758 hasConcept C112613896 @default.
- W2963323758 hasConcept C178790620 @default.
- W2963323758 hasConcept C185592680 @default.
- W2963323758 hasConcept C22814914 @default.
- W2963323758 hasConcept C32264632 @default.
- W2963323758 hasConcept C521977710 @default.
- W2963323758 hasConcept C55493867 @default.
- W2963323758 hasConceptScore W2963323758C112613896 @default.
- W2963323758 hasConceptScore W2963323758C178790620 @default.
- W2963323758 hasConceptScore W2963323758C185592680 @default.
- W2963323758 hasConceptScore W2963323758C22814914 @default.
- W2963323758 hasConceptScore W2963323758C32264632 @default.
- W2963323758 hasConceptScore W2963323758C521977710 @default.
- W2963323758 hasConceptScore W2963323758C55493867 @default.
- W2963323758 hasIssue "10" @default.
- W2963323758 hasLocation W29633237581 @default.
- W2963323758 hasLocation W29633237582 @default.
- W2963323758 hasOpenAccess W2963323758 @default.
- W2963323758 hasPrimaryLocation W29633237581 @default.
- W2963323758 hasRelatedWork W1155390618 @default.
- W2963323758 hasRelatedWork W1754259525 @default.
- W2963323758 hasRelatedWork W2001564713 @default.
- W2963323758 hasRelatedWork W2020942986 @default.
- W2963323758 hasRelatedWork W2023127335 @default.
- W2963323758 hasRelatedWork W2025935935 @default.
- W2963323758 hasRelatedWork W2033602362 @default.
- W2963323758 hasRelatedWork W2065806513 @default.
- W2963323758 hasRelatedWork W2105497666 @default.
- W2963323758 hasRelatedWork W2952305790 @default.
- W2963323758 hasVolume "49" @default.
- W2963323758 isParatext "false" @default.
- W2963323758 isRetracted "false" @default.
- W2963323758 magId "2963323758" @default.
- W2963323758 workType "article" @default.