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- W2963454071 abstract "Abstract The present study describes the isolation of the alkaloid “antofine” from Cynanchum atratum BUNGE as well as its anti-fungal potential against the citrus postharvest pathogen Penicillium digitatum. Antofine, which was extracted using an ultrasonic-assisted method and identified by 1H, 13C-NMR, and LC/MS analysis, showed strong antifungal activity against P. digitatum, with an observed minimum inhibitory concentration (MIC) and a minimum fungicidal concentration (MFC) of 1.56 × 10−3 and 1.25 × 10-2 g L-1, respectively. In vivo assays showed that antofine could significantly reduce the incidence of green mold. Furthermore, the total lipid and ergosterol contents of P. digitatum decreased after antofine treatment, indicating the disruption of membrane integrity. In addition, antofine caused a significant reduction of the intracellular adenosine triphosphate (ATP) content during the entire exposure period. These results suggest that the antifungal activity of antofine against P. digitatum can be attributed to the disruption of the cell membrane integrity and energy metabolism." @default.
- W2963454071 created "2019-07-30" @default.
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- W2963454071 date "2019-11-01" @default.
- W2963454071 modified "2023-09-30" @default.
- W2963454071 title "Isolation of antofine from Cynanchum atratum BUNGE (Asclepiadaceae) and its antifungal activity against Penicillium digitatum" @default.
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- W2963454071 doi "https://doi.org/10.1016/j.postharvbio.2019.110961" @default.
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