Matches in SemOpenAlex for { <https://semopenalex.org/work/W2963472015> ?p ?o ?g. }
- W2963472015 endingPage "6916" @default.
- W2963472015 startingPage "6900" @default.
- W2963472015 abstract "Abstract Antisense technology can reduce gene expression via the RNase H1 or RISC pathways and can increase gene expression through modulation of splicing or translation. Here, we demonstrate that antisense oligonucleotides (ASOs) can reduce mRNA levels by acting through the no-go decay pathway. Phosphorothioate ASOs fully modified with 2′-O-methoxyethyl decreased mRNA levels when targeted to coding regions of mRNAs in a translation-dependent, RNase H1-independent manner. The ASOs that activated this decay pathway hybridized near the 3′ end of the coding regions. Although some ASOs induced nonsense-mediated decay, others reduced mRNA levels through the no-go decay pathway, since depletion of PELO/HBS1L, proteins required for no-go decay pathway activity, decreased the activities of these ASOs. ASO length and chemical modification influenced the efficacy of these reagents. This non-gapmer ASO-induced mRNA reduction was observed for different transcripts and in different cell lines. Thus, our study identifies a new mechanism by which mRNAs can be degraded using ASOs, adding a new antisense approach to modulation of gene expression. It also helps explain why some fully modified ASOs cause RNA target to be reduced despite being unable to serve as substrates for RNase H1." @default.
- W2963472015 created "2019-07-30" @default.
- W2963472015 creator A5035551086 @default.
- W2963472015 creator A5046729344 @default.
- W2963472015 creator A5050786473 @default.
- W2963472015 creator A5070182884 @default.
- W2963472015 creator A5077755519 @default.
- W2963472015 date "2019-06-05" @default.
- W2963472015 modified "2023-09-30" @default.
- W2963472015 title "mRNA levels can be reduced by antisense oligonucleotides via no-go decay pathway" @default.
- W2963472015 cites W1820845817 @default.
- W2963472015 cites W1972453061 @default.
- W2963472015 cites W1985013843 @default.
- W2963472015 cites W1986704873 @default.
- W2963472015 cites W1990570801 @default.
- W2963472015 cites W1993987952 @default.
- W2963472015 cites W1996288085 @default.
- W2963472015 cites W2002336825 @default.
- W2963472015 cites W2005153622 @default.
- W2963472015 cites W2006898408 @default.
- W2963472015 cites W2011405092 @default.
- W2963472015 cites W2012893162 @default.
- W2963472015 cites W2019764744 @default.
- W2963472015 cites W2020917719 @default.
- W2963472015 cites W2025722577 @default.
- W2963472015 cites W2039742014 @default.
- W2963472015 cites W2041583688 @default.
- W2963472015 cites W2042335793 @default.
- W2963472015 cites W2045653120 @default.
- W2963472015 cites W2055234488 @default.
- W2963472015 cites W2058745674 @default.
- W2963472015 cites W2064808830 @default.
- W2963472015 cites W2081452698 @default.
- W2963472015 cites W2088685757 @default.
- W2963472015 cites W2088860412 @default.
- W2963472015 cites W2090183104 @default.
- W2963472015 cites W2092964977 @default.
- W2963472015 cites W2096688350 @default.
- W2963472015 cites W2099602353 @default.
- W2963472015 cites W2100682779 @default.
- W2963472015 cites W2102846253 @default.
- W2963472015 cites W2112909190 @default.
- W2963472015 cites W2117759290 @default.
- W2963472015 cites W2120624230 @default.
- W2963472015 cites W2124444021 @default.
- W2963472015 cites W2130886644 @default.
- W2963472015 cites W2135566609 @default.
- W2963472015 cites W2148310540 @default.
- W2963472015 cites W2154134651 @default.
- W2963472015 cites W2160395592 @default.
- W2963472015 cites W2190875401 @default.
- W2963472015 cites W2256185547 @default.
- W2963472015 cites W2261840374 @default.
- W2963472015 cites W2318775098 @default.
- W2963472015 cites W2463620300 @default.
- W2963472015 cites W2464724577 @default.
- W2963472015 cites W2471690043 @default.
- W2963472015 cites W2496954959 @default.
- W2963472015 cites W2507852077 @default.
- W2963472015 cites W2515806738 @default.
- W2963472015 cites W2550329754 @default.
- W2963472015 cites W2564732169 @default.
- W2963472015 cites W2591728558 @default.
- W2963472015 cites W2624035866 @default.
- W2963472015 cites W2737645474 @default.
- W2963472015 cites W2743879871 @default.
- W2963472015 cites W2758757342 @default.
- W2963472015 cites W2770649267 @default.
- W2963472015 cites W2783437035 @default.
- W2963472015 cites W2789935006 @default.
- W2963472015 cites W2795531081 @default.
- W2963472015 cites W2883705358 @default.
- W2963472015 cites W2889214184 @default.
- W2963472015 cites W2891665599 @default.
- W2963472015 cites W2895639919 @default.
- W2963472015 cites W2902094095 @default.
- W2963472015 doi "https://doi.org/10.1093/nar/gkz500" @default.
- W2963472015 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6649848" @default.
- W2963472015 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31165876" @default.
- W2963472015 hasPublicationYear "2019" @default.
- W2963472015 type Work @default.
- W2963472015 sameAs 2963472015 @default.
- W2963472015 citedByCount "27" @default.
- W2963472015 countsByYear W29634720152019 @default.
- W2963472015 countsByYear W29634720152020 @default.
- W2963472015 countsByYear W29634720152021 @default.
- W2963472015 countsByYear W29634720152022 @default.
- W2963472015 countsByYear W29634720152023 @default.
- W2963472015 crossrefType "journal-article" @default.
- W2963472015 hasAuthorship W2963472015A5035551086 @default.
- W2963472015 hasAuthorship W2963472015A5046729344 @default.
- W2963472015 hasAuthorship W2963472015A5050786473 @default.
- W2963472015 hasAuthorship W2963472015A5070182884 @default.
- W2963472015 hasAuthorship W2963472015A5077755519 @default.
- W2963472015 hasBestOaLocation W29634720151 @default.
- W2963472015 hasConcept C104317684 @default.
- W2963472015 hasConcept C105580179 @default.
- W2963472015 hasConcept C10879258 @default.