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• W2964000558 abstract "The performance of a bioreactor in meeting process goals is affected by the microorganism used, medium composition, and operating conditions. A typical bioreactor uses a supervisory control and data acquisition (SCADA) system for control, and a combination of software and hardware tools for real‐time data analysis. However, when the process is disrupted by utility or instrumentation failure, typical process controllers may be unable to reinstate normal operating conditions before the cells in the reactor shift to unfavorable metabolic regimes. The objective of this study is to examine how the response of a controller affects process recovery when disruptive incidences occur under a process analytical technology (PAT) framework. The process used for this investigation is the production of lethal toxin‐neutralizing factor (LTNF) by Escherichia coli ( E. coli ), which is controlled by a decoupled input–output‐linearizing controller (DIOLC). The performance of the DIOLC is compared to a proportional integral derivative (PID) controller subjected to the same conditions. The disruptions are introduced manually and the effect of controller action on process recovery and LTNF synthesis is measured in terms of peak purity and concentration. It is observed that DIOLC performs better after reinstating operating conditions and results in a meaningful improvement in performance." @default.
• W2964000558 created "2019-07-30" @default.
• W2964000558 creator A5067986166 @default.
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• W2964000558 date "2019-08-21" @default.
• W2964000558 modified "2023-09-29" @default.
• W2964000558 title "Implementing Process Analytical Technology for the Production of Recombinant Proteins in <i>Escherichia coli</i> Using an Advanced Controller Scheme" @default.
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• W2964000558 doi "https://doi.org/10.1002/biot.201800556" @default.
• W2964000558 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31329337" @default.
• W2964000558 hasPublicationYear "2019" @default.
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