Matches in SemOpenAlex for { <https://semopenalex.org/work/W2964223721> ?p ?o ?g. }
- W2964223721 endingPage "1060" @default.
- W2964223721 startingPage "1039" @default.
- W2964223721 abstract "Endothelial cells exposed to the Random Positioning Machine (RPM) reveal three different phenotypes. They grow as a two-dimensional monolayer and form three-dimensional (3D) structures such as spheroids and tubular constructs. As part of the ESA-SPHEROIDS project we want to understand how endothelial cells (ECs) react and adapt to long-term microgravity.During a spaceflight to the International Space Station (ISS) and a subsequent stay onboard, human ECs (EA.hy926 cell line) were cultured for 12 days in real microgravity inside an automatic flight hardware, specially designed for use in space. ECs were cultivated in the absence or presence of vascular endothelial growth factor, which had demonstrated a cell-protective effect on ECs exposed to an RPM simulating microgravity. After cell fixation in space and return of the samples, we examined cell morphology and analyzed supernatants by Multianalyte Profiling technology.The fixed samples comprised 3D multicellular spheroids and tube-like structures in addition to monolayer cells, which are exclusively observed during growth under Earth gravity (1g). Within the 3D aggregates we detected enhanced collagen and laminin. The supernatant analysis unveiled alterations in secretion of several growth factors, cytokines, and extracellular matrix components as compared to cells cultivated at 1g or on the RPM. This confirmed an influence of gravity on interacting key proteins and genes and demonstrated a flight hardware impact on the endothelial secretome.Since formation of tube-like aggregates was observed only on the RPM and during spaceflight, we conclude that microgravity may be the major cause for ECs' 3D aggregation." @default.
- W2964223721 created "2019-07-30" @default.
- W2964223721 creator A5000871147 @default.
- W2964223721 creator A5002710339 @default.
- W2964223721 creator A5003737733 @default.
- W2964223721 creator A5010781386 @default.
- W2964223721 creator A5015354144 @default.
- W2964223721 creator A5017701621 @default.
- W2964223721 creator A5019078437 @default.
- W2964223721 creator A5022582588 @default.
- W2964223721 creator A5057342865 @default.
- W2964223721 creator A5063366433 @default.
- W2964223721 creator A5064608729 @default.
- W2964223721 creator A5071324256 @default.
- W2964223721 creator A5071661821 @default.
- W2964223721 creator A5081634976 @default.
- W2964223721 creator A5082120013 @default.
- W2964223721 creator A5083626058 @default.
- W2964223721 creator A5085500296 @default.
- W2964223721 creator A5091820377 @default.
- W2964223721 date "2019-04-17" @default.
- W2964223721 modified "2023-10-14" @default.
- W2964223721 title "Growth of Endothelial Cells in Space and in Simulated Microgravity – a Comparison on the Secretory Level" @default.
- W2964223721 cites W1564674105 @default.
- W2964223721 cites W1966911838 @default.
- W2964223721 cites W1969721216 @default.
- W2964223721 cites W1975964490 @default.
- W2964223721 cites W1979058246 @default.
- W2964223721 cites W1979165962 @default.
- W2964223721 cites W1982370874 @default.
- W2964223721 cites W1986117422 @default.
- W2964223721 cites W1986189363 @default.
- W2964223721 cites W1988678004 @default.
- W2964223721 cites W1993246046 @default.
- W2964223721 cites W1994061930 @default.
- W2964223721 cites W1997866086 @default.
- W2964223721 cites W1999223106 @default.
- W2964223721 cites W2001993791 @default.
- W2964223721 cites W2006234418 @default.
- W2964223721 cites W2018616527 @default.
- W2964223721 cites W2032165714 @default.
- W2964223721 cites W2033358042 @default.
- W2964223721 cites W2037548392 @default.
- W2964223721 cites W2041834530 @default.
- W2964223721 cites W2048563410 @default.
- W2964223721 cites W2049136921 @default.
- W2964223721 cites W2049827480 @default.
- W2964223721 cites W2056339467 @default.
- W2964223721 cites W2059944938 @default.
- W2964223721 cites W2061437659 @default.
- W2964223721 cites W2067908965 @default.
- W2964223721 cites W2071184715 @default.
- W2964223721 cites W2106307355 @default.
- W2964223721 cites W2109324313 @default.
- W2964223721 cites W2115327466 @default.
- W2964223721 cites W2126087366 @default.
- W2964223721 cites W2127675271 @default.
- W2964223721 cites W2127839969 @default.
- W2964223721 cites W2128829295 @default.
- W2964223721 cites W2143893042 @default.
- W2964223721 cites W2157970156 @default.
- W2964223721 cites W2165093995 @default.
- W2964223721 cites W2229718138 @default.
- W2964223721 cites W2234818479 @default.
- W2964223721 cites W2267250079 @default.
- W2964223721 cites W2280511049 @default.
- W2964223721 cites W2287987188 @default.
- W2964223721 cites W2396879256 @default.
- W2964223721 cites W2444353434 @default.
- W2964223721 cites W2480872114 @default.
- W2964223721 cites W2546877613 @default.
- W2964223721 cites W2551907224 @default.
- W2964223721 cites W2587458433 @default.
- W2964223721 cites W263695075 @default.
- W2964223721 cites W2772241505 @default.
- W2964223721 cites W2793262993 @default.
- W2964223721 cites W2804632664 @default.
- W2964223721 cites W2899728927 @default.
- W2964223721 doi "https://doi.org/10.33594/000000071" @default.
- W2964223721 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30977987" @default.
- W2964223721 hasPublicationYear "2019" @default.
- W2964223721 type Work @default.
- W2964223721 sameAs 2964223721 @default.
- W2964223721 citedByCount "31" @default.
- W2964223721 countsByYear W29642237212019 @default.
- W2964223721 countsByYear W29642237212020 @default.
- W2964223721 countsByYear W29642237212021 @default.
- W2964223721 countsByYear W29642237212022 @default.
- W2964223721 countsByYear W29642237212023 @default.
- W2964223721 crossrefType "journal-article" @default.
- W2964223721 hasAuthorship W2964223721A5000871147 @default.
- W2964223721 hasAuthorship W2964223721A5002710339 @default.
- W2964223721 hasAuthorship W2964223721A5003737733 @default.
- W2964223721 hasAuthorship W2964223721A5010781386 @default.
- W2964223721 hasAuthorship W2964223721A5015354144 @default.
- W2964223721 hasAuthorship W2964223721A5017701621 @default.
- W2964223721 hasAuthorship W2964223721A5019078437 @default.
- W2964223721 hasAuthorship W2964223721A5022582588 @default.