Matches in SemOpenAlex for { <https://semopenalex.org/work/W2964232129> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W2964232129 abstract "Mesenchymal stem/stromal cells (MSCs) are of significant clinical interest in regenerative medicine because of their trilineage potential to differentiate into cells integral for tissues such as bone and cartilage. Current therapies for tissue damage often require surgical intervention, which has drawbacks including invasiveness and susceptibility to infection. Stem cell-based therapies likely offer a more natural alternative to repair damaged tissue. Nanoparticles are a promising tool for biomedical applications, especially as a vehicle for biomolecule delivery when coated with temperature-responsive polymers to entrap and release biomolecules, such as proteins, in a triggered manner. Superparamagnetic iron oxide nanoparticles (SPIONs) can undergo a phenomenon termed magnetic heating in the presence of an alternating magnetic field. SPIONs surface-functionalised with temperature-responsive polymers present a potential combination for protein delivery and release.In this study, I report the development of SPIONs coated with temperature-sensitive polymer poly(N-isopropylacrylamide) (PNIPAM). PNIPAM-coated SPIONs showed temperature-sensitive properties above the lower critical solution temperature (LCST) of PNIPAM (32oC) when heated with or without magnetic heating. I could characterise the protein encapsulation and release of test protein apotransferrin from PNIPAM-coated nanoparticles in both a pH- and temperature-sensitive manner. Magnetic heating, though a constant or pulsed application, was sufficient to trigger the release of encapsulated apotransferrin from PNIPAM-coated nanoparticles, where switching off the magnetic field halted further protein release. Magnetically-triggered Wnt3a release from PNIPAM-coated nanoparticles elicited cellular activation through the enhanced green fluorescent protein (eGFP) response of an MSC Wnt reporter line, as well as increased proliferation of both immortalised and primary MSCs. I also developed temperature-responsive polymer, poly(N-isopropylmethacrylamide) (PNIPMAM), which exhibited a higher LCST than PNIPAM (42oC) and could release apotransferrin above physiological temperature with/without magnetic heating.The developed temperature and magnetically-responsive SPIONs could be a potential tool to promote MSC-driven tissue regeneration through biomolecule delivery and temperature-sensitive release." @default.
- W2964232129 created "2019-07-30" @default.
- W2964232129 creator A5030631041 @default.
- W2964232129 date "2018-12-01" @default.
- W2964232129 modified "2023-09-27" @default.
- W2964232129 title "Development of functional nanoparticles for in situ tissue engineering" @default.
- W2964232129 hasPublicationYear "2018" @default.
- W2964232129 type Work @default.
- W2964232129 sameAs 2964232129 @default.
- W2964232129 citedByCount "0" @default.
- W2964232129 crossrefType "dissertation" @default.
- W2964232129 hasAuthorship W2964232129A5030631041 @default.
- W2964232129 hasConcept C115260700 @default.
- W2964232129 hasConcept C121332964 @default.
- W2964232129 hasConcept C12554922 @default.
- W2964232129 hasConcept C136229726 @default.
- W2964232129 hasConcept C15083742 @default.
- W2964232129 hasConcept C155672457 @default.
- W2964232129 hasConcept C15920480 @default.
- W2964232129 hasConcept C159985019 @default.
- W2964232129 hasConcept C171250308 @default.
- W2964232129 hasConcept C188109995 @default.
- W2964232129 hasConcept C191897082 @default.
- W2964232129 hasConcept C192562407 @default.
- W2964232129 hasConcept C198826908 @default.
- W2964232129 hasConcept C23792430 @default.
- W2964232129 hasConcept C26926545 @default.
- W2964232129 hasConcept C2777230088 @default.
- W2964232129 hasConcept C2778889443 @default.
- W2964232129 hasConcept C32546565 @default.
- W2964232129 hasConcept C49853544 @default.
- W2964232129 hasConcept C49892992 @default.
- W2964232129 hasConcept C521977710 @default.
- W2964232129 hasConcept C62520636 @default.
- W2964232129 hasConcept C71924100 @default.
- W2964232129 hasConcept C86803240 @default.
- W2964232129 hasConcept C87023908 @default.
- W2964232129 hasConcept C95444343 @default.
- W2964232129 hasConceptScore W2964232129C115260700 @default.
- W2964232129 hasConceptScore W2964232129C121332964 @default.
- W2964232129 hasConceptScore W2964232129C12554922 @default.
- W2964232129 hasConceptScore W2964232129C136229726 @default.
- W2964232129 hasConceptScore W2964232129C15083742 @default.
- W2964232129 hasConceptScore W2964232129C155672457 @default.
- W2964232129 hasConceptScore W2964232129C15920480 @default.
- W2964232129 hasConceptScore W2964232129C159985019 @default.
- W2964232129 hasConceptScore W2964232129C171250308 @default.
- W2964232129 hasConceptScore W2964232129C188109995 @default.
- W2964232129 hasConceptScore W2964232129C191897082 @default.
- W2964232129 hasConceptScore W2964232129C192562407 @default.
- W2964232129 hasConceptScore W2964232129C198826908 @default.
- W2964232129 hasConceptScore W2964232129C23792430 @default.
- W2964232129 hasConceptScore W2964232129C26926545 @default.
- W2964232129 hasConceptScore W2964232129C2777230088 @default.
- W2964232129 hasConceptScore W2964232129C2778889443 @default.
- W2964232129 hasConceptScore W2964232129C32546565 @default.
- W2964232129 hasConceptScore W2964232129C49853544 @default.
- W2964232129 hasConceptScore W2964232129C49892992 @default.
- W2964232129 hasConceptScore W2964232129C521977710 @default.
- W2964232129 hasConceptScore W2964232129C62520636 @default.
- W2964232129 hasConceptScore W2964232129C71924100 @default.
- W2964232129 hasConceptScore W2964232129C86803240 @default.
- W2964232129 hasConceptScore W2964232129C87023908 @default.
- W2964232129 hasConceptScore W2964232129C95444343 @default.
- W2964232129 hasLocation W29642321291 @default.
- W2964232129 hasOpenAccess W2964232129 @default.
- W2964232129 hasPrimaryLocation W29642321291 @default.
- W2964232129 hasRelatedWork W1971137527 @default.
- W2964232129 hasRelatedWork W2006404487 @default.
- W2964232129 hasRelatedWork W2048444554 @default.
- W2964232129 hasRelatedWork W2075181486 @default.
- W2964232129 hasRelatedWork W2090831791 @default.
- W2964232129 hasRelatedWork W2148015157 @default.
- W2964232129 hasRelatedWork W2245319613 @default.
- W2964232129 hasRelatedWork W2290614006 @default.
- W2964232129 hasRelatedWork W2752348755 @default.
- W2964232129 hasRelatedWork W2773792462 @default.
- W2964232129 hasRelatedWork W2781377740 @default.
- W2964232129 hasRelatedWork W2788717070 @default.
- W2964232129 hasRelatedWork W2789545916 @default.
- W2964232129 hasRelatedWork W2843219477 @default.
- W2964232129 hasRelatedWork W2895043124 @default.
- W2964232129 hasRelatedWork W2897632940 @default.
- W2964232129 hasRelatedWork W2906590014 @default.
- W2964232129 hasRelatedWork W2990270639 @default.
- W2964232129 hasRelatedWork W2991587522 @default.
- W2964232129 hasRelatedWork W3025120326 @default.
- W2964232129 isParatext "false" @default.
- W2964232129 isRetracted "false" @default.
- W2964232129 magId "2964232129" @default.
- W2964232129 workType "dissertation" @default.