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- W2964507788 abstract "Abstract CRISPR/Cas9 is a powerful genome editing tool that has been successfully applied to a variety of species, including zebrafish. However, targeting efficiencies vary greatly at different genomic loci, the underlying causes of which were still elusive. Here we report a quick CRISPR/Cas9 system, designated as qCas9, which exhibits accelerated turnover of Cas9 protein in zebrafish. Our data showed that qCas9 significantly improved targeting efficiency, including both knock-out and knock-in in F 0 embryos, and yielded higher germline transmission rate in founder screen. Importantly, qCas9 showed little to no off-target editing in zebrafish and profoundly reduced off-target effect in HEK293T cell line. In summary, our findings demonstrate that qCas9 is a simple, economic and highly effective method to improve genome editing efficiency in zebrafish embryos and also holds great potential in reducing off-target effect in mammalian cell lines." @default.
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- W2964507788 date "2019-08-02" @default.
- W2964507788 modified "2023-09-25" @default.
- W2964507788 title "Enhance genome editing efficiency and specificity by a quick CRISPR/Cas9 system" @default.
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- W2964507788 doi "https://doi.org/10.1101/708404" @default.
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