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- W2964508863 abstract "目的建立高血压患者血浆肾素活性(plasma renin activity, PRA)液相色谱-串联质谱(liquid chromatography-tandem mass spectrometry, LC-MS/MS)的检测方法, 用于早期筛查高血压患者中的原发性醛固酮增多症(primary aldosteronism, PA)。 方法arginine 13C 15N作为内标, 采用Phenomenex Kinetex C18柱(2.6 μm, 100 mm×3.0 mm)进行分离, 柱温55 ℃。流动相为0.2%甲醇水溶液和0.2%甲酸甲醇溶液, 流速为0.5 mL/min, 梯度洗脱。考察该方法的特异性、基质效应、携带污染、重复性、定量下限(lower limit of measuring interval, LLMI)、线性、不精密度、回收率、稀释一致性和血浆标本稳定性。采用LC-MS/MS方法测定296名表面健康人群(男性153名, 女性143名, 平均年龄48岁)及360名高血压患者(平均年龄52岁, 确诊为PA 27例)的血浆样本PRA, 并与放射免疫法(radioimmunoassay, RIA)进行比较。 结果该方法通过了特异性、基质效应、携带污染、重复性的性能评价。PRA的检测线性范围为0.084 4~30.000 0 ng/mL; PRA的LLMI为0.084 4 ng·mL-1·h-1; 日间和批内的不精密度变异系数(coefficient of variation, CV)均＜15%;准确度偏差均 结论我们建立了准确检测PRA的LC-MS/MS方法, 适合于临床早期快速筛查PA。" @default.
- W2964508863 created "2019-08-13" @default.
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- W2964508863 date "2019-01-25" @default.
- W2964508863 modified "2023-09-27" @default.
- W2964508863 title "液相色谱-串联质谱(LC-MS/MS)检测血浆肾素活性(PRA)的方法学建立和临床应用评估" @default.
- W2964508863 doi "https://doi.org/10.3969/j.issn.1672-8467.2019.01.012" @default.
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