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• W2964669097 endingPage "3794" @default.
• W2964669097 startingPage "3786" @default.
• W2964669097 abstract "Abstract Microchimerism is defined as the presence of a small population of cells or DNA in 1 organism originated from a genetically different organism. It is well established that this phenomenon occurs in humans and mice as cells are exchanged between mother and fetus during gestation. Currently, no information is available about the presence of maternal microchimerism in goats, and the only published study is limited to an evaluation of fetal and fetal–fetal microchimerism in blood samples following natural breeding. In order to determine whether bidirectional fetal–maternal cell or DNA trafficking occurs in goats, we assessed: 1) fetal microchimerism in surrogates that gave birth to somatic cell nuclear transfer (SCNT)-derived transgenic offspring (n = 4), 2) maternal microchimerism following natural breeding of SCNT-derived transgenic does with a nontransgenic buck (n = 4), and 3) fetal–fetal microchimerism in nontransgenic twins of transgenic offspring (n = 3). Neomycin-resistance gene (NEO) gene was selected as the marker to detect the presence of the αMHC-TGF-β1-Neo transgene in kidney, liver, lung, lymph node, and spleen. We found no detectable maternal or fetal–fetal microchimerism in the investigated tissues of nontransgenic offspring. However, fetal microchimerism was detected in lymph node tissue of one of the surrogate dams carrying a SCNT pregnancy. These results indicate occurrence of cell trafficking from fetus to mother during SCNT pregnancies. The findings of this study have direct implications on the use and disposal of nontransgenic surrogates and nontransgenic offspring." @default.
• W2964669097 created "2019-08-13" @default.
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• W2964669097 date "2019-07-29" @default.
• W2964669097 modified "2023-09-27" @default.
• W2964669097 title "Assessment of microchimerism following somatic cell nuclear transfer and natural pregnancies in goats" @default.
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• W2964669097 doi "https://doi.org/10.1093/jas/skz248" @default.
• W2964669097 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6735858" @default.
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