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- W2964673514 endingPage "492" @default.
- W2964673514 startingPage "492" @default.
- W2964673514 abstract "Carbon nanotubes (CNTs) have already been considered for medical applications due to their small diameter and ability to penetrate cells and tissues. However, since CNTs are chemically inert and non-dispersible in water, they have to be chemically functionalized or coated with biomolecules to carry payloads or interact with the environment. Proteins, although often only randomly bound to the CNT surface, are preferred because they provide a better biocompatibility and present functional groups for binding additional molecules. A new approach to functionalize CNTs with a closed and precisely ordered protein layer is offered by bacterial surface layer (S-layer) proteins, which have already attracted much attention in the functionalization of surfaces. We could demonstrate that bacterial S-layer proteins (SbpA of Lysinibacillus sphaericus CCM 2177 and the recombinant fusion protein rSbpA31-1068GG comprising the S-layer protein and two copies of the IgG binding region of Protein G) can be used to disperse and functionalize oxidized multi walled CNTs. Following a simple protocol, a complete surface coverage with a long-range crystalline S-layer lattice can be obtained. When rSbpA31-1068GG was used for coating, the introduced functionality could be confirmed by binding gold labeled antibodies via the IgG binding domain of the fusion protein. Since a great variety of functional S-layer fusion proteins has already been described, our new technology has the potential for a broad spectrum of functionalized CNTs." @default.
- W2964673514 created "2019-08-13" @default.
- W2964673514 creator A5020717643 @default.
- W2964673514 creator A5027486450 @default.
- W2964673514 creator A5052082810 @default.
- W2964673514 creator A5056053675 @default.
- W2964673514 creator A5081559582 @default.
- W2964673514 date "2019-08-02" @default.
- W2964673514 modified "2023-09-27" @default.
- W2964673514 title "S-Layer Protein Coated Carbon Nanotubes" @default.
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- W2964673514 doi "https://doi.org/10.3390/coatings9080492" @default.
- W2964673514 hasPublicationYear "2019" @default.
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