FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2965167496> ?p ?o ?g. }

• W2965167496 endingPage "214" @default.
• W2965167496 startingPage "214" @default.
• W2965167496 abstract "939 Currently, most methods available to study the behavior of metastatic tumor cells are indirect or capture only brief periods. Problematic with these assays are an inability to follow the complete metastatic program including extravasation, invasion, establishment and growth of the metastatic mass. As such, we are developing an ex vivo model in which tumor cells can be analyzed while in the environment of a major metastatic target, the liver. In this study we assessed whether prostate cancer metastasis, which is among the leading causes of deaths in American males in the United States, could be recreated in a liver bioreactor and observed in real time. In order to accomplish this we utilized a three dimensional liver perfusion culture (bioreactor), which allows for in situ observation and ensures a relatively homogeneous distribution of flow and mass transfer throughout the system to meet the metabolic demands of the livers cells augmented by an appropriate scaffold which facilitates morphogenesis of primary cells into tissue-like structures. This system, formally named a Micro-fabricated Array Bioreactor, affords for the recreation of an in vivo environment for in vitro observation and provides for an optimal device for the study of physiological events. Hepatocytes and nonparenchymal liver cells were obtained and introduced into the bioreactor utilizing established protocols. After allowing 5 days of observed hepatic tissue morphogenesis, DU-145 human prostate carcinoma cells expressing GFP or RFP were introduced into the bioreactor. In situ observation of the co-culture system was observed by fluorescence microscopy over a 30 day period. The rate of growth of prostate cancer cells was assessed with a custom designed spectofluorometer to measure GFP fluorescent intensity throughout the initial growth of the cancer. Tissue architecture of hepatocytes and prostate cancer cells was directly visualized in real time by 2-photon microscopy along with light microscopy. Fluorescent intensity of co-cultures revealed ongoing cell proliferation of the DU-145 cells, which correlated with a visually observed overgrowth of DU-145 cells by 14 days. DU-145 WT cells failed to grow without hepatocytes or even in a 2-D mixed culture. This tumor mass growth resulted in a decline in hepatocyte tissue structure and function. Also metastasis of overflowing DU-145 cells to adjacent hepatocyte filled channels not containing DU-WT cells was observed during this process by 14 days. Histological examination of the tissue revealed DU-145 WT cells invading the hepatocyte parenchyma by 15 days. Finally, biochemical analysis revealed expression of biomarkers associated with prostate cancer and decrease in albumin and urea by the hepatocytes. Although only preliminary, these experiments provide the basis for the development of and ex vivo model system in which to observe and potentially dissect the dynamic process of tumor invasion and metastasis." @default.
• W2965167496 created "2019-08-13" @default.
• W2965167496 creator A5030935360 @default.
• W2965167496 creator A5036021081 @default.
• W2965167496 creator A5060515951 @default.
• W2965167496 creator A5074553698 @default.
• W2965167496 date "2004-04-01" @default.
• W2965167496 modified "2023-09-24" @default.
• W2965167496 title "Direct visualization of prostate cancer progression utilizing a bioreactor" @default.
• W2965167496 hasPublicationYear "2004" @default.
• W2965167496 type Work @default.
• W2965167496 sameAs 2965167496 @default.
• W2965167496 citedByCount "0" @default.
• W2965167496 crossrefType "journal-article" @default.
• W2965167496 hasAuthorship W2965167496A5030935360 @default.
• W2965167496 hasAuthorship W2965167496A5036021081 @default.
• W2965167496 hasAuthorship W2965167496A5060515951 @default.
• W2965167496 hasAuthorship W2965167496A5074553698 @default.
• W2965167496 hasConcept C104317684 @default.
• W2965167496 hasConcept C121608353 @default.
• W2965167496 hasConcept C126322002 @default.
• W2965167496 hasConcept C136229726 @default.
• W2965167496 hasConcept C142724271 @default.
• W2965167496 hasConcept C150903083 @default.
• W2965167496 hasConcept C168170006 @default.
• W2965167496 hasConcept C185592680 @default.
• W2965167496 hasConcept C207001950 @default.
• W2965167496 hasConcept C26291073 @default.
• W2965167496 hasConcept C2776235491 @default.
• W2965167496 hasConcept C2779013556 @default.
• W2965167496 hasConcept C2779540182 @default.
• W2965167496 hasConcept C2780192828 @default.
• W2965167496 hasConcept C40692019 @default.
• W2965167496 hasConcept C502942594 @default.
• W2965167496 hasConcept C55493867 @default.
• W2965167496 hasConcept C59822182 @default.
• W2965167496 hasConcept C71924100 @default.
• W2965167496 hasConcept C86803240 @default.
• W2965167496 hasConcept C95444343 @default.
• W2965167496 hasConcept C96232424 @default.
• W2965167496 hasConceptScore W2965167496C104317684 @default.
• W2965167496 hasConceptScore W2965167496C121608353 @default.
• W2965167496 hasConceptScore W2965167496C126322002 @default.
• W2965167496 hasConceptScore W2965167496C136229726 @default.
• W2965167496 hasConceptScore W2965167496C142724271 @default.
• W2965167496 hasConceptScore W2965167496C150903083 @default.
• W2965167496 hasConceptScore W2965167496C168170006 @default.
• W2965167496 hasConceptScore W2965167496C185592680 @default.
• W2965167496 hasConceptScore W2965167496C207001950 @default.
• W2965167496 hasConceptScore W2965167496C26291073 @default.
• W2965167496 hasConceptScore W2965167496C2776235491 @default.
• W2965167496 hasConceptScore W2965167496C2779013556 @default.
• W2965167496 hasConceptScore W2965167496C2779540182 @default.
• W2965167496 hasConceptScore W2965167496C2780192828 @default.
• W2965167496 hasConceptScore W2965167496C40692019 @default.
• W2965167496 hasConceptScore W2965167496C502942594 @default.
• W2965167496 hasConceptScore W2965167496C55493867 @default.
• W2965167496 hasConceptScore W2965167496C59822182 @default.
• W2965167496 hasConceptScore W2965167496C71924100 @default.
• W2965167496 hasConceptScore W2965167496C86803240 @default.
• W2965167496 hasConceptScore W2965167496C95444343 @default.
• W2965167496 hasConceptScore W2965167496C96232424 @default.
• W2965167496 hasLocation W29651674961 @default.
• W2965167496 hasOpenAccess W2965167496 @default.
• W2965167496 hasPrimaryLocation W29651674961 @default.
• W2965167496 hasRelatedWork W1566557693 @default.
• W2965167496 hasRelatedWork W1656855425 @default.
• W2965167496 hasRelatedWork W1842829551 @default.
• W2965167496 hasRelatedWork W1973363476 @default.
• W2965167496 hasRelatedWork W1977457442 @default.
• W2965167496 hasRelatedWork W2005054620 @default.
• W2965167496 hasRelatedWork W2034045048 @default.
• W2965167496 hasRelatedWork W2034728603 @default.
• W2965167496 hasRelatedWork W2113562053 @default.
• W2965167496 hasRelatedWork W2114700145 @default.
• W2965167496 hasRelatedWork W214482553 @default.
• W2965167496 hasRelatedWork W2161204618 @default.
• W2965167496 hasRelatedWork W253376117 @default.
• W2965167496 hasRelatedWork W2885314438 @default.
• W2965167496 hasRelatedWork W2912505823 @default.
• W2965167496 hasRelatedWork W295701813 @default.
• W2965167496 hasRelatedWork W2977234576 @default.
• W2965167496 hasRelatedWork W3163960205 @default.
• W2965167496 hasRelatedWork W32851924 @default.
• W2965167496 hasRelatedWork W2849001644 @default.
• W2965167496 hasVolume "64" @default.
• W2965167496 isParatext "false" @default.
• W2965167496 isRetracted "false" @default.
• W2965167496 magId "2965167496" @default.
• W2965167496 workType "article" @default.