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- W2965217680 abstract "This protocol describes a method to deplete and repopulate organotypic hippocampal slice cultures with ramified microglia. We describe the slice culture preparation from newborn mice, standard culturing of neonatal microglia, and the acute isolation of microglia from adult mouse brain. Furthermore, we outline the technique for the replenishment of microglia-depleted slice cultures with different microglia populations and subsequent morphological analysis. We show that neonatal and adult microglia acquire specific ramified morphologies, which in case of adult microglia are indistinguishable from the in vivo situation. This procedure not only allows the functional investigation of microglia with different degrees of ramification but also enables the construction of chimeric slice cultures with respect to the microglia phenotype. Preparation of slice cultures can be completed in 3.5 h, preparation of mixed-glial cultures in 4 h, isolation of adult microglia can be accomplished in 3.5 h, and replenishment in 30 min." @default.
- W2965217680 created "2019-08-13" @default.
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- W2965217680 date "2019-01-01" @default.
- W2965217680 modified "2023-10-17" @default.
- W2965217680 title "Replenishment of Organotypic Hippocampal Slice Cultures with Neonatal or Adult Microglia" @default.
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- W2965217680 doi "https://doi.org/10.1007/978-1-4939-9658-2_10" @default.
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