FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2965353907> ?p ?o ?g. }

• W2965353907 endingPage "109237" @default.
• W2965353907 startingPage "109237" @default.
• W2965353907 abstract "Tea consumption has positive effects on the skeletal system and prevents postmenopausal osteoporosis, mainly by inhibiting osteoclastogenesis. In green tea, (-)-epigallocatechin-3-gallate (EGCG) is the most abundant and active compound and has been shown to inhibit RANKL-induced osteoclast formation. Taking into account the highly oxidizable and unstable nature of EGCG, we hypothesized that EGCG oxidation product exhibits greater anti-osteoclastogenesis potential than EGCG. In this study, we successfully isolated and identified an EGCG oxidation derivative, (-)-gallocatechin gallate (compound 2), using a chemical oxidation strategy. We then compared the ability of compound 2 and EGCG to inhibit RANKL-induced osteoclastogenesis in RAW 264.7 cells. The results of TRAP staining and F-actin ring immunofluorescent staining showed that compound 2 exhibits stronger inhibition of RANKL-induced osteoclast differentiation and F-actin ring formation, respectively, than EGCG. Additionally, quantitative real-time PCR (qRT-PCR) and western blotting analyses showed that compound 2 significantly and more strongly inhibited the expression of osteoclastogenesis-related marker genes and proteins, including c-Src, TRAP, cathepsin K, β3-Integrin, and MMP-9, compared with EGCG. Furthermore, compound 2 significantly suppressed RANKL-induced expression of NFATc1 and c-Fos, the master transcriptional regulators of osteoclastogenesis, more strongly than EGCG. Mechanistically, molecular interaction assays showed that compound 2 binds to RANK with high affinity (KD = 189 nM) and blocks RANKL-RANK interactions, thereby suppressing RANKL-induced early RANK signaling pathways including p65, JNK, ERK, and p38 in osteoclast precursors. Taken together, this study demonstrates for the first time that an oxidation derivative of EGCG (compound 2) inhibits RANKL-induced osteoclastogenesis by suppressing RANK signaling pathways in RAW 264.7 cells." @default.
• W2965353907 created "2019-08-13" @default.
• W2965353907 creator A5001424435 @default.
• W2965353907 creator A5002270191 @default.
• W2965353907 creator A5029636776 @default.
• W2965353907 creator A5054709496 @default.
• W2965353907 creator A5056101783 @default.
• W2965353907 creator A5060640690 @default.
• W2965353907 creator A5066448103 @default.
• W2965353907 creator A5087929515 @default.
• W2965353907 creator A5089130857 @default.
• W2965353907 creator A5090916655 @default.
• W2965353907 date "2019-10-01" @default.
• W2965353907 modified "2023-10-16" @default.
• W2965353907 title "Oxidation derivative of (-)-epigallocatechin-3-gallate (EGCG) inhibits RANKL-induced osteoclastogenesis by suppressing RANK signaling pathways in RAW 264.7 cells" @default.
• W2965353907 cites W1591981746 @default.
• W2965353907 cites W1971672495 @default.
• W2965353907 cites W1992393816 @default.
• W2965353907 cites W2038466470 @default.
• W2965353907 cites W2056944100 @default.
• W2965353907 cites W2057820671 @default.
• W2965353907 cites W2079173609 @default.
• W2965353907 cites W2093252878 @default.
• W2965353907 cites W2094983273 @default.
• W2965353907 cites W2096542991 @default.
• W2965353907 cites W2104743116 @default.
• W2965353907 cites W2109934063 @default.
• W2965353907 cites W2116597451 @default.
• W2965353907 cites W2118983568 @default.
• W2965353907 cites W2403659169 @default.
• W2965353907 cites W2529535353 @default.
• W2965353907 cites W2618213894 @default.
• W2965353907 cites W2743187300 @default.
• W2965353907 cites W2765893332 @default.
• W2965353907 cites W2770826431 @default.
• W2965353907 cites W2783192114 @default.
• W2965353907 cites W2790471362 @default.
• W2965353907 cites W2795906441 @default.
• W2965353907 cites W2810284427 @default.
• W2965353907 cites W2883463467 @default.
• W2965353907 cites W2902172467 @default.
• W2965353907 cites W2902806889 @default.
• W2965353907 cites W2905663314 @default.
• W2965353907 cites W2907689514 @default.
• W2965353907 cites W2916044106 @default.
• W2965353907 cites W2916484820 @default.
• W2965353907 cites W2918049346 @default.
• W2965353907 cites W2943335832 @default.
• W2965353907 doi "https://doi.org/10.1016/j.biopha.2019.109237" @default.
• W2965353907 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31376653" @default.
• W2965353907 hasPublicationYear "2019" @default.
• W2965353907 type Work @default.
• W2965353907 sameAs 2965353907 @default.
• W2965353907 citedByCount "19" @default.
• W2965353907 countsByYear W29653539072020 @default.
• W2965353907 countsByYear W29653539072021 @default.
• W2965353907 countsByYear W29653539072022 @default.
• W2965353907 countsByYear W29653539072023 @default.
• W2965353907 crossrefType "journal-article" @default.
• W2965353907 hasAuthorship W2965353907A5001424435 @default.
• W2965353907 hasAuthorship W2965353907A5002270191 @default.
• W2965353907 hasAuthorship W2965353907A5029636776 @default.
• W2965353907 hasAuthorship W2965353907A5054709496 @default.
• W2965353907 hasAuthorship W2965353907A5056101783 @default.
• W2965353907 hasAuthorship W2965353907A5060640690 @default.
• W2965353907 hasAuthorship W2965353907A5066448103 @default.
• W2965353907 hasAuthorship W2965353907A5087929515 @default.
• W2965353907 hasAuthorship W2965353907A5089130857 @default.
• W2965353907 hasAuthorship W2965353907A5090916655 @default.
• W2965353907 hasBestOaLocation W29653539071 @default.
• W2965353907 hasConcept C101990758 @default.
• W2965353907 hasConcept C134018914 @default.
• W2965353907 hasConcept C153911025 @default.
• W2965353907 hasConcept C170493617 @default.
• W2965353907 hasConcept C185592680 @default.
• W2965353907 hasConcept C2776033226 @default.
• W2965353907 hasConcept C2779428903 @default.
• W2965353907 hasConcept C55493867 @default.
• W2965353907 hasConcept C57074206 @default.
• W2965353907 hasConcept C62478195 @default.
• W2965353907 hasConcept C673006 @default.
• W2965353907 hasConcept C86803240 @default.
• W2965353907 hasConcept C88045685 @default.
• W2965353907 hasConcept C95444343 @default.
• W2965353907 hasConceptScore W2965353907C101990758 @default.
• W2965353907 hasConceptScore W2965353907C134018914 @default.
• W2965353907 hasConceptScore W2965353907C153911025 @default.
• W2965353907 hasConceptScore W2965353907C170493617 @default.
• W2965353907 hasConceptScore W2965353907C185592680 @default.
• W2965353907 hasConceptScore W2965353907C2776033226 @default.
• W2965353907 hasConceptScore W2965353907C2779428903 @default.
• W2965353907 hasConceptScore W2965353907C55493867 @default.
• W2965353907 hasConceptScore W2965353907C57074206 @default.
• W2965353907 hasConceptScore W2965353907C62478195 @default.
• W2965353907 hasConceptScore W2965353907C673006 @default.
• W2965353907 hasConceptScore W2965353907C86803240 @default.
• W2965353907 hasConceptScore W2965353907C88045685 @default.
• W2965353907 hasConceptScore W2965353907C95444343 @default.

• next