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• W2965486591 abstract "Currently, gold nanoparticles have found applications in engineering and medical sciences, taking advantage from their properties and characteristics. Surface plasmon resonance, for instance, is one of the main features for optical applications and other physical properties, like high density, that represents the key for cellular uptake. Among other applications, in the medical field, some diseases may be treated by using gene therapy, including monogenetic or polygenetic disorders and infections. Gene adding, suppression, or substitution is one of the many options for genetic manipulation. This work explores an alternative non-viral method for gene transfer by using gold nanoparticles functionalized with organic polymers; two routes of synthesis were used: one of them with sodium borohydride as reducing agent and the other one with chitosan oligosaccharide as reducing and stabilizing agent. Gold nanoparticles conjugated with chitosan, acylated chitosan and chitosan oligosaccharide, were used to evaluate transfection efficiency of plasmid DNA into cell culture (HEK-293). Physical and chemical properties of gold nanocomposites were characterized by using UV-Vis Spectroscopy, ξ-potential, and transmission electron microscopy. Furthermore, the interaction between gold nanoparticles and plasmid DNA was demonstrated by using agarose gel electrophoresis. Transfection tests were performed and evaluated by β-galactosidase activity and green fluorescence protein expression. The percentage of transfection obtained with chitosan, acylated chitosan, and chitosan oligosaccharide were of 27%, 33%, and 60% respectively." @default.
• W2965486591 created "2019-08-13" @default.
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• W2965486591 date "2019-07-30" @default.
• W2965486591 modified "2023-10-13" @default.
• W2965486591 title "Gold nanoparticles with chitosan, N-acylated chitosan, and chitosan oligosaccharide as DNA carriers" @default.
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• W2965486591 doi "https://doi.org/10.1186/s11671-019-3083-y" @default.
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• W2965486591 hasPublicationYear "2019" @default.
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