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• W2965512306 endingPage "e1008305" @default.
• W2965512306 startingPage "e1008305" @default.
• W2965512306 abstract "C-to-U editing is an important event in post-transcriptional RNA processing, which converts a specific cytidine (C)-to-uridine (U) in transcripts of mitochondria and plastids. Typically, the pentatricopeptide repeat (PPR) protein, which specifies the target C residue by binding to its upstream sequence, is involved in the editing of one or a few sites. Here we report a novel PPR-DYW protein EMP21 that is associated with editing of 81 sites in maize. EMP21 is localized in mitochondria and loss of the EMP21 function severely inhibits the embryogenesis and endosperm development in maize. From a scan of 35 mitochondrial transcripts produced by the Emp21 loss-of-function mutant, the C-to-U editing was found to be abolished at five sites (nad7-77, atp1-1292, atp8-437, nad3-275 and rps4-870), while reduced at 76 sites in 21 transcripts. In most cases, the failure to editing resulted in the translation of an incorrect residue. In consequence, the mutant became deficient with respect to the assembly and activity of mitochondrial complexes I and V. As six of the decreased editing sites in emp21 overlap with the affected editing sites in emp5-1, and the editing efficiency at rpl16-458 showed a substantial reduction in the emp21-1 emp5-4 double mutant compared with the emp21-1 and emp5-4 single mutants, we explored their interaction. A yeast two hybrid assay suggested that EMP21 does not interact with EMP5, but both EMP21 and EMP5 interact with ZmMORF8. Together, these results indicate that EMP21 is a novel PPR-DYW protein required for the editing of ~17% of mitochondrial target Cs, and the editing process may involve an interaction between EMP21 and ZmMORF8 (and probably other proteins)." @default.
• W2965512306 created "2019-08-13" @default.
• W2965512306 creator A5018332487 @default.
• W2965512306 creator A5034661086 @default.
• W2965512306 creator A5034676224 @default.
• W2965512306 creator A5039469426 @default.
• W2965512306 creator A5053501929 @default.
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• W2965512306 creator A5066102428 @default.
• W2965512306 creator A5072274470 @default.
• W2965512306 creator A5073223092 @default.
• W2965512306 creator A5086172861 @default.
• W2965512306 date "2019-08-02" @default.
• W2965512306 modified "2023-10-14" @default.
• W2965512306 title "Empty Pericarp21 encodes a novel PPR-DYW protein that is required for mitochondrial RNA editing at multiple sites, complexes I and V biogenesis, and seed development in maize" @default.
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