FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2965588750> ?p ?o ?g. }

• W2965588750 endingPage "267" @default.
• W2965588750 startingPage "258" @default.
• W2965588750 abstract "Microbial conversions known as “biological funneling” have attracted attention for their ability to upgrade heterogeneous mixtures of low-molecular-weight aromatic compounds obtained by chemical lignin depolymerization. β-hydroxypropiovanillone (HPV) and its analogs can be obtained by chemoselective catalytic oxidation of lignin using 2,3-dichloro-5,6-dicyano-1,4-benzoquinone/tert-butyl nitrite/O2, followed by cleavage of arylglycerol-β-aryl ether with zinc. Sphingobium sp. strain SYK-6 can degrade HPV generated by the catabolism of arylglycerol-β-aryl ether through 2-pyrone-4,6-dicarboxylate (PDC), a promising platform chemical. Therefore, production of PDC from HPV can be achieved using the HPV catabolic pathway. However, the pathway and genes involved in the catabolism of vanilloyl acetic acid (VAA) generated during HPV catabolism have not been investigated. In the present study, we isolated SLG_24960 (vceA), which encodes an enzyme that converts VAA into a coenzyme A (CoA) derivative of vanillate (vanilloyl-CoA) from SYK-6, by shotgun cloning. The analysis of a vceA mutant indicated that this gene is not required for VAA conversion in vivo, but it encodes a major enzyme catalyzing CoA-dependent VAA conversion in vitro. We also identified SLG_12450 (vceB), whose product can convert vanilloyl-CoA to vanillate. Enzyme genes besides vceA and vceB, which are necessary for the conversions of HPV to VAA and of vanillate to PDC, were introduced and expressed in Pseudomonas putida. The resulting engineered strain completely converted 1 mM HPV into PDC after 24 h. Our results suggest that the enzyme genes that are not required for the catabolic pathway in microorganisms but can be used for the conversion of target substrates are buried in microbial genomes. These genes are, thus, useful for designing metabolic pathways to produce value-added metabolites." @default.
• W2965588750 created "2019-08-13" @default.
• W2965588750 creator A5017202324 @default.
• W2965588750 creator A5040036023 @default.
• W2965588750 creator A5053831224 @default.
• W2965588750 creator A5062345002 @default.
• W2965588750 creator A5067260525 @default.
• W2965588750 creator A5068773653 @default.
• W2965588750 date "2019-09-01" @default.
• W2965588750 modified "2023-09-25" @default.
• W2965588750 title "Discovery of novel enzyme genes involved in the conversion of an arylglycerol-β-aryl ether metabolite and their use in generating a metabolic pathway for lignin valorization" @default.
• W2965588750 cites W1484562275 @default.
• W2965588750 cites W1502250996 @default.
• W2965588750 cites W1507332308 @default.
• W2965588750 cites W1970051672 @default.
• W2965588750 cites W1971144082 @default.
• W2965588750 cites W1984865044 @default.
• W2965588750 cites W1986106976 @default.
• W2965588750 cites W1987355768 @default.
• W2965588750 cites W1994961051 @default.
• W2965588750 cites W2005291574 @default.
• W2965588750 cites W2007972307 @default.
• W2965588750 cites W2014745341 @default.
• W2965588750 cites W2015260661 @default.
• W2965588750 cites W2021015961 @default.
• W2965588750 cites W2021435901 @default.
• W2965588750 cites W2046036240 @default.
• W2965588750 cites W2047360542 @default.
• W2965588750 cites W2060684323 @default.
• W2965588750 cites W2074976215 @default.
• W2965588750 cites W2082907400 @default.
• W2965588750 cites W2087756365 @default.
• W2965588750 cites W2095900397 @default.
• W2965588750 cites W2107214348 @default.
• W2965588750 cites W2108067031 @default.
• W2965588750 cites W2116189397 @default.
• W2965588750 cites W2119151987 @default.
• W2965588750 cites W2119273703 @default.
• W2965588750 cites W2121541027 @default.
• W2965588750 cites W2127322768 @default.
• W2965588750 cites W2129443011 @default.
• W2965588750 cites W2146341019 @default.
• W2965588750 cites W2150646586 @default.
• W2965588750 cites W2156663168 @default.
• W2965588750 cites W2159435901 @default.
• W2965588750 cites W2161321889 @default.
• W2965588750 cites W2170639649 @default.
• W2965588750 cites W2294693772 @default.
• W2965588750 cites W2326700708 @default.
• W2965588750 cites W2400171832 @default.
• W2965588750 cites W2528599146 @default.
• W2965588750 cites W2551952924 @default.
• W2965588750 cites W2653547707 @default.
• W2965588750 cites W2770114257 @default.
• W2965588750 cites W2786479777 @default.
• W2965588750 cites W2791774752 @default.
• W2965588750 cites W2887408513 @default.
• W2965588750 cites W2917405238 @default.
• W2965588750 cites W2945910415 @default.
• W2965588750 cites W4242137597 @default.
• W2965588750 doi "https://doi.org/10.1016/j.ymben.2019.08.002" @default.
• W2965588750 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31390538" @default.
• W2965588750 hasPublicationYear "2019" @default.
• W2965588750 type Work @default.
• W2965588750 sameAs 2965588750 @default.
• W2965588750 citedByCount "22" @default.
• W2965588750 countsByYear W29655887502019 @default.
• W2965588750 countsByYear W29655887502020 @default.
• W2965588750 countsByYear W29655887502021 @default.
• W2965588750 countsByYear W29655887502022 @default.
• W2965588750 countsByYear W29655887502023 @default.
• W2965588750 crossrefType "journal-article" @default.
• W2965588750 hasAuthorship W2965588750A5017202324 @default.
• W2965588750 hasAuthorship W2965588750A5040036023 @default.
• W2965588750 hasAuthorship W2965588750A5053831224 @default.
• W2965588750 hasAuthorship W2965588750A5062345002 @default.
• W2965588750 hasAuthorship W2965588750A5067260525 @default.
• W2965588750 hasAuthorship W2965588750A5068773653 @default.
• W2965588750 hasBestOaLocation W29655887502 @default.
• W2965588750 hasConcept C181199279 @default.
• W2965588750 hasConcept C185592680 @default.
• W2965588750 hasConcept C192989942 @default.
• W2965588750 hasConcept C2777271787 @default.
• W2965588750 hasConcept C2777477808 @default.
• W2965588750 hasConcept C2780543182 @default.
• W2965588750 hasConcept C2781066024 @default.
• W2965588750 hasConcept C55493867 @default.
• W2965588750 hasConcept C6350086 @default.
• W2965588750 hasConcept C86803240 @default.
• W2965588750 hasConcept C96942376 @default.
• W2965588750 hasConceptScore W2965588750C181199279 @default.
• W2965588750 hasConceptScore W2965588750C185592680 @default.
• W2965588750 hasConceptScore W2965588750C192989942 @default.
• W2965588750 hasConceptScore W2965588750C2777271787 @default.
• W2965588750 hasConceptScore W2965588750C2777477808 @default.
• W2965588750 hasConceptScore W2965588750C2780543182 @default.
• W2965588750 hasConceptScore W2965588750C2781066024 @default.
• W2965588750 hasConceptScore W2965588750C55493867 @default.

• next