Matches in SemOpenAlex for { <https://semopenalex.org/work/W2965725837> ?p ?o ?g. }
- W2965725837 endingPage "3656" @default.
- W2965725837 startingPage "3656" @default.
- W2965725837 abstract "Unlike canonical phytochromes, the GAF domain of cyanobacteriochromes (CBCRs) can bind bilins autonomously and is sufficient for functional photocycles. Despite the astonishing spectral diversity of CBCRs, the GAF1 domain of the three-GAF-domain photoreceptor all2699 from the cyanobacterium Nostoc 7120 is the only CBCR-GAF known that converts from a red-absorbing (Pr) dark state to a far-red-absorbing (Pfr) photoproduct, analogous to the more conservative phytochromes. Here we report a solid-state NMR spectroscopic study of all2699g1 in its Pr state. Conclusive NMR evidence unveils a particular stereochemical heterogeneity at the tetrahedral C31 atom, whereas the crystal structure shows exclusively the R-stereochemistry at this chiral center. Additional NMR experiments were performed on a construct comprising the GAF1 and GAF2 domains of all2699, showing a greater precision in the chromophore-protein interactions in the GAF1-2 construct. A 3D Pr structural model of the all2699g1-2 construct predicts a tongue-like region extending from the GAF2 domain (akin to canonical phytochromes) in the direction of the chromophore, shielding it from the solvent. In addition, this stabilizing element allows exclusively the R-stereochemistry for the chromophore-protein linkage. Site-directed mutagenesis performed on three conserved motifs in the hairpin-like tip confirms the interaction of the tongue region with the GAF1-bound chromophore." @default.
- W2965725837 created "2019-08-13" @default.
- W2965725837 creator A5005845793 @default.
- W2965725837 creator A5010086053 @default.
- W2965725837 creator A5011470329 @default.
- W2965725837 creator A5011884895 @default.
- W2965725837 creator A5036968154 @default.
- W2965725837 creator A5039597828 @default.
- W2965725837 creator A5040909240 @default.
- W2965725837 creator A5050848884 @default.
- W2965725837 creator A5056035382 @default.
- W2965725837 date "2019-07-26" @default.
- W2965725837 modified "2023-09-29" @default.
- W2965725837 title "MAS NMR on a Red/Far-Red Photochromic Cyanobacteriochrome All2699 from Nostoc" @default.
- W2965725837 cites W1061988093 @default.
- W2965725837 cites W1492498352 @default.
- W2965725837 cites W1967124494 @default.
- W2965725837 cites W1971857987 @default.
- W2965725837 cites W1971944356 @default.
- W2965725837 cites W1973987642 @default.
- W2965725837 cites W1978856722 @default.
- W2965725837 cites W1981589073 @default.
- W2965725837 cites W1982896956 @default.
- W2965725837 cites W1985426903 @default.
- W2965725837 cites W1999850786 @default.
- W2965725837 cites W2003537892 @default.
- W2965725837 cites W2004166665 @default.
- W2965725837 cites W2006608901 @default.
- W2965725837 cites W2009769433 @default.
- W2965725837 cites W2015844604 @default.
- W2965725837 cites W2020572633 @default.
- W2965725837 cites W2020720377 @default.
- W2965725837 cites W2024487526 @default.
- W2965725837 cites W2026108112 @default.
- W2965725837 cites W2036903393 @default.
- W2965725837 cites W2037392653 @default.
- W2965725837 cites W2043049548 @default.
- W2965725837 cites W2046072513 @default.
- W2965725837 cites W2046939723 @default.
- W2965725837 cites W2050903472 @default.
- W2965725837 cites W2057663339 @default.
- W2965725837 cites W2059930259 @default.
- W2965725837 cites W2064922129 @default.
- W2965725837 cites W2067412481 @default.
- W2965725837 cites W2071197565 @default.
- W2965725837 cites W2071803420 @default.
- W2965725837 cites W2083374498 @default.
- W2965725837 cites W2090924962 @default.
- W2965725837 cites W2100475854 @default.
- W2965725837 cites W2106549599 @default.
- W2965725837 cites W2106965096 @default.
- W2965725837 cites W2112095150 @default.
- W2965725837 cites W2117696710 @default.
- W2965725837 cites W2129917646 @default.
- W2965725837 cites W2130662176 @default.
- W2965725837 cites W2131034797 @default.
- W2965725837 cites W2143721066 @default.
- W2965725837 cites W2149525061 @default.
- W2965725837 cites W2155106146 @default.
- W2965725837 cites W2163280837 @default.
- W2965725837 cites W2164389392 @default.
- W2965725837 cites W2235657626 @default.
- W2965725837 cites W2265014908 @default.
- W2965725837 cites W2314645623 @default.
- W2965725837 cites W2315319832 @default.
- W2965725837 cites W2322461264 @default.
- W2965725837 cites W2332740526 @default.
- W2965725837 cites W2335118584 @default.
- W2965725837 cites W2335436651 @default.
- W2965725837 cites W2429925263 @default.
- W2965725837 cites W2557026901 @default.
- W2965725837 cites W2565289937 @default.
- W2965725837 cites W2572080171 @default.
- W2965725837 cites W2611585618 @default.
- W2965725837 cites W2796751041 @default.
- W2965725837 cites W2796790787 @default.
- W2965725837 cites W2801175205 @default.
- W2965725837 cites W2806884083 @default.
- W2965725837 cites W2810322934 @default.
- W2965725837 cites W2891074931 @default.
- W2965725837 cites W2899784956 @default.
- W2965725837 cites W2901424771 @default.
- W2965725837 cites W2911998268 @default.
- W2965725837 cites W2920230103 @default.
- W2965725837 cites W2988989751 @default.
- W2965725837 doi "https://doi.org/10.3390/ijms20153656" @default.
- W2965725837 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6696110" @default.
- W2965725837 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31357417" @default.
- W2965725837 hasPublicationYear "2019" @default.
- W2965725837 type Work @default.
- W2965725837 sameAs 2965725837 @default.
- W2965725837 citedByCount "13" @default.
- W2965725837 countsByYear W29657258372020 @default.
- W2965725837 countsByYear W29657258372021 @default.
- W2965725837 countsByYear W29657258372022 @default.
- W2965725837 countsByYear W29657258372023 @default.
- W2965725837 crossrefType "journal-article" @default.
- W2965725837 hasAuthorship W2965725837A5005845793 @default.