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• W2966289645 abstract "<ns4:p>Nicastrin (NCSTN) is a transmembrane glycoprotein that is part of the gamma-secretase complex. Gamma-secretase is a protease complex that cleaves type-I single-pass transmembrane proteins. There are many potential substrates for this complex, including NOTCH receptors and amyloid precursor proteins (APP). There are a number of commercial antibodies to nicastrin, but they do not agree on expected peptide size. We confirmed the specificity of a C-terminal binding rabbit anti-human antibody from Sigma-Aldrich (#N1660) using wildtype HEK293 cells and HEK293 cells deleted for nicastrin. The wildtype cells showed a prominent band at approximately 110 kDa. We confirmed this larger than expected sized was due to glycosylation by treating the lysate with peptide-N-glycosidase F (PNGase F), which reduced the band to less than 75 kDa. These data suggest that this polyclonal is specific for nicastrin and can detect endogenous levels of protein.</ns4:p>" @default.
• W2966289645 created "2019-08-13" @default.
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• W2966289645 date "2020-01-10" @default.
• W2966289645 modified "2023-09-24" @default.
• W2966289645 title "Validation of a commercial antibody to detect endogenous human nicastrin by immunoblot" @default.
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• W2966289645 doi "https://doi.org/10.12688/f1000research.19803.2" @default.
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