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• W2966610985 abstract "Genetically encoded calcium indicators (GECIs) have become widely used for Ca2+ imaging in cultured cells as well as in living organisms. Transduction of microglia with viral vectors encoding GECIs provides a convenient means to label microglia for in vivo Ca2+ imaging. We describe a method using microglia-specific microRNA-9-regulated viral vector, to label microglial cells with a ratiometric GECI (Twitch-2B). This method enables longitudinal recording of both transient and sustained elevations of Ca2+ in microglia in live animals." @default.
• W2966610985 created "2019-08-13" @default.
• W2966610985 creator A5019498638 @default.
• W2966610985 creator A5026285776 @default.
• W2966610985 date "2019-01-01" @default.
• W2966610985 modified "2023-10-07" @default.
• W2966610985 title "Labeling Microglia with Genetically Encoded Calcium Indicators" @default.
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• W2966610985 doi "https://doi.org/10.1007/978-1-4939-9658-2_18" @default.
• W2966610985 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31392690" @default.
• W2966610985 hasPublicationYear "2019" @default.
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