Matches in SemOpenAlex for { <https://semopenalex.org/work/W2966702973> ?p ?o ?g. }
- W2966702973 endingPage "878" @default.
- W2966702973 startingPage "867" @default.
- W2966702973 abstract "In this work, we studied the expression kinetics and innate immune response of a self-amplifying mRNA (sa-RNA) after electroporation and lipid-nanoparticle (LNP)-mediated delivery in the skin of mice. Intradermal electroporation of the sa-RNA resulted in a plateau-shaped expression, with the plateau between day 3 and day 10. The overall protein expression of sa-RNA was significantly higher than that obtained after electroporation of plasmid DNA (pDNA) or non-replication mRNAs. Moreover, using IFN-β reporter mice, we elucidated that intradermal electroporation of sa-RNA induced a short-lived moderate innate immune response, which did not affect the expression of the sa-RNA. A completely different expression profile and innate immune response were observed when LNPs were used. The expression peaked 24 h after intradermal injection of sa-RNA-LNPs and subsequently showed a sharp drop. This drop might be explained by a translational blockage caused by the strong innate immune response that we observed in IFN-β reporter mice shortly (4 h) after intradermal injection of sa-RNA-LNPs. A final interesting observation was the capacity of sa-RNA-LNPs to transfect the draining lymph nodes after intradermal injection." @default.
- W2966702973 created "2019-08-13" @default.
- W2966702973 creator A5016436400 @default.
- W2966702973 creator A5024611033 @default.
- W2966702973 creator A5032337906 @default.
- W2966702973 creator A5048595477 @default.
- W2966702973 creator A5057456976 @default.
- W2966702973 creator A5064040222 @default.
- W2966702973 creator A5064161466 @default.
- W2966702973 creator A5076510895 @default.
- W2966702973 creator A5090598261 @default.
- W2966702973 date "2019-09-01" @default.
- W2966702973 modified "2023-10-14" @default.
- W2966702973 title "Expression Kinetics and Innate Immune Response after Electroporation and LNP-Mediated Delivery of a Self-Amplifying mRNA in the Skin" @default.
- W2966702973 cites W1215958058 @default.
- W2966702973 cites W1420726270 @default.
- W2966702973 cites W1523792532 @default.
- W2966702973 cites W1855484651 @default.
- W2966702973 cites W191828198 @default.
- W2966702973 cites W1973966982 @default.
- W2966702973 cites W1979434879 @default.
- W2966702973 cites W1982091532 @default.
- W2966702973 cites W1984497743 @default.
- W2966702973 cites W1986094246 @default.
- W2966702973 cites W1987728748 @default.
- W2966702973 cites W1990093169 @default.
- W2966702973 cites W2016327770 @default.
- W2966702973 cites W2018368471 @default.
- W2966702973 cites W2033787149 @default.
- W2966702973 cites W2037988139 @default.
- W2966702973 cites W2042249918 @default.
- W2966702973 cites W2051665875 @default.
- W2966702973 cites W2054298563 @default.
- W2966702973 cites W2057402777 @default.
- W2966702973 cites W2061742276 @default.
- W2966702973 cites W2071302752 @default.
- W2966702973 cites W2078896740 @default.
- W2966702973 cites W2094780294 @default.
- W2966702973 cites W2130397544 @default.
- W2966702973 cites W2131349128 @default.
- W2966702973 cites W2138088412 @default.
- W2966702973 cites W2139665761 @default.
- W2966702973 cites W2153049488 @default.
- W2966702973 cites W2153264881 @default.
- W2966702973 cites W2160632084 @default.
- W2966702973 cites W2162970812 @default.
- W2966702973 cites W2163659675 @default.
- W2966702973 cites W2164150624 @default.
- W2966702973 cites W2249755146 @default.
- W2966702973 cites W2285232484 @default.
- W2966702973 cites W2412881016 @default.
- W2966702973 cites W2437830629 @default.
- W2966702973 cites W2474379397 @default.
- W2966702973 cites W2485031226 @default.
- W2966702973 cites W2515313544 @default.
- W2966702973 cites W2521176812 @default.
- W2966702973 cites W2549555716 @default.
- W2966702973 cites W2564638240 @default.
- W2966702973 cites W2567689582 @default.
- W2966702973 cites W2587719281 @default.
- W2966702973 cites W2587871737 @default.
- W2966702973 cites W2593990758 @default.
- W2966702973 cites W2610471486 @default.
- W2966702973 cites W2738515079 @default.
- W2966702973 cites W2754401101 @default.
- W2966702973 cites W2781711484 @default.
- W2966702973 cites W2782708334 @default.
- W2966702973 cites W2790311195 @default.
- W2966702973 cites W2801179771 @default.
- W2966702973 cites W2802041199 @default.
- W2966702973 cites W2901779366 @default.
- W2966702973 cites W2955806244 @default.
- W2966702973 doi "https://doi.org/10.1016/j.omtn.2019.08.001" @default.
- W2966702973 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6722285" @default.
- W2966702973 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31472371" @default.
- W2966702973 hasPublicationYear "2019" @default.
- W2966702973 type Work @default.
- W2966702973 sameAs 2966702973 @default.
- W2966702973 citedByCount "38" @default.
- W2966702973 countsByYear W29667029732019 @default.
- W2966702973 countsByYear W29667029732020 @default.
- W2966702973 countsByYear W29667029732021 @default.
- W2966702973 countsByYear W29667029732022 @default.
- W2966702973 countsByYear W29667029732023 @default.
- W2966702973 crossrefType "journal-article" @default.
- W2966702973 hasAuthorship W2966702973A5016436400 @default.
- W2966702973 hasAuthorship W2966702973A5024611033 @default.
- W2966702973 hasAuthorship W2966702973A5032337906 @default.
- W2966702973 hasAuthorship W2966702973A5048595477 @default.
- W2966702973 hasAuthorship W2966702973A5057456976 @default.
- W2966702973 hasAuthorship W2966702973A5064040222 @default.
- W2966702973 hasAuthorship W2966702973A5064161466 @default.
- W2966702973 hasAuthorship W2966702973A5076510895 @default.
- W2966702973 hasAuthorship W2966702973A5090598261 @default.
- W2966702973 hasBestOaLocation W29667029731 @default.
- W2966702973 hasConcept C104317684 @default.
- W2966702973 hasConcept C105580179 @default.
- W2966702973 hasConcept C136449434 @default.