Matches in SemOpenAlex for { <https://semopenalex.org/work/W2966964169> ?p ?o ?g. }
- W2966964169 endingPage "e0220416" @default.
- W2966964169 startingPage "e0220416" @default.
- W2966964169 abstract "Notophthalmus viridescens (Red-spotted Newt) possess amazing capabilities to regenerate their organs and other tissues. Previously, using a de novo assembly of the newt transcriptome combined with proteomic validation, our group identified a novel family of five protein members expressed in adult tissues during regeneration in Notophthalmus viridescens. The presence of a putative signal peptide suggests that all these proteins are secretory in nature. Here we employed iterative threading assembly refinement (I-TASSER) server to generate three-dimensional structure of these novel Newt proteins and predicted their function. Our data suggests that these proteins could act as ion transporters, and be involved in redox reaction(s). Due to absence of transgenic approaches in N. viridescens, and conservation of genetic machinery across species, we generated transgenic Drosophila melanogaster to misexpress these genes. Expression of 2775 transcripts were compared between these five newly identified Newt genes. We found that genes involved in the developmental process, cell cycle, apoptosis, and immune response are among those that are highly enriched. To validate the RNA Seq. data, expression of six highly regulated genes were verified using real time Quantitative Polymerase Chain Reaction (RT-qPCR). These graded gene expression patterns provide insight into the function of novel protein family identified in Newt, and layout a map for future studies in the field." @default.
- W2966964169 created "2019-08-22" @default.
- W2966964169 creator A5034790064 @default.
- W2966964169 creator A5055410869 @default.
- W2966964169 creator A5063022537 @default.
- W2966964169 creator A5067603018 @default.
- W2966964169 creator A5076998130 @default.
- W2966964169 date "2019-08-16" @default.
- W2966964169 modified "2023-10-14" @default.
- W2966964169 title "Comparative transcriptomic analysis and structure prediction of novel Newt proteins" @default.
- W2966964169 cites W107544894 @default.
- W2966964169 cites W1606812730 @default.
- W2966964169 cites W1697278489 @default.
- W2966964169 cites W1799760597 @default.
- W2966964169 cites W1843970493 @default.
- W2966964169 cites W1854617081 @default.
- W2966964169 cites W1939805691 @default.
- W2966964169 cites W1969353942 @default.
- W2966964169 cites W1974765744 @default.
- W2966964169 cites W1978717530 @default.
- W2966964169 cites W1980642761 @default.
- W2966964169 cites W1984282087 @default.
- W2966964169 cites W1987041209 @default.
- W2966964169 cites W1987725525 @default.
- W2966964169 cites W1987992613 @default.
- W2966964169 cites W1991049879 @default.
- W2966964169 cites W1992663358 @default.
- W2966964169 cites W2018938817 @default.
- W2966964169 cites W2026827807 @default.
- W2966964169 cites W2029155007 @default.
- W2966964169 cites W2034962885 @default.
- W2966964169 cites W2038963685 @default.
- W2966964169 cites W2043162569 @default.
- W2966964169 cites W2047762794 @default.
- W2966964169 cites W2055961987 @default.
- W2966964169 cites W2059515441 @default.
- W2966964169 cites W2060939114 @default.
- W2966964169 cites W2069235384 @default.
- W2966964169 cites W2086651895 @default.
- W2966964169 cites W2088398273 @default.
- W2966964169 cites W2098764861 @default.
- W2966964169 cites W2100448568 @default.
- W2966964169 cites W2103121176 @default.
- W2966964169 cites W2104582907 @default.
- W2966964169 cites W2106029142 @default.
- W2966964169 cites W2109348183 @default.
- W2966964169 cites W2110643873 @default.
- W2966964169 cites W2121859376 @default.
- W2966964169 cites W2125349610 @default.
- W2966964169 cites W2126194853 @default.
- W2966964169 cites W2127181940 @default.
- W2966964169 cites W2129149076 @default.
- W2966964169 cites W2131254563 @default.
- W2966964169 cites W2131719579 @default.
- W2966964169 cites W2140729960 @default.
- W2966964169 cites W2141595533 @default.
- W2966964169 cites W2141652419 @default.
- W2966964169 cites W2148627988 @default.
- W2966964169 cites W2152239989 @default.
- W2966964169 cites W2154431984 @default.
- W2966964169 cites W2159935247 @default.
- W2966964169 cites W2165464596 @default.
- W2966964169 cites W2165606488 @default.
- W2966964169 cites W2170396116 @default.
- W2966964169 cites W2180909065 @default.
- W2966964169 cites W2266510186 @default.
- W2966964169 cites W2279177954 @default.
- W2966964169 cites W2402793346 @default.
- W2966964169 cites W2463932398 @default.
- W2966964169 cites W2516522777 @default.
- W2966964169 cites W2530158410 @default.
- W2966964169 cites W2580555388 @default.
- W2966964169 cites W2592701059 @default.
- W2966964169 cites W2598282452 @default.
- W2966964169 cites W2604098124 @default.
- W2966964169 cites W2748775553 @default.
- W2966964169 cites W2763440940 @default.
- W2966964169 cites W2776469698 @default.
- W2966964169 cites W2789590942 @default.
- W2966964169 cites W2797456644 @default.
- W2966964169 cites W2800092422 @default.
- W2966964169 cites W2801674050 @default.
- W2966964169 cites W2802996424 @default.
- W2966964169 cites W2890187632 @default.
- W2966964169 cites W2896274905 @default.
- W2966964169 cites W2897677237 @default.
- W2966964169 cites W2899590661 @default.
- W2966964169 cites W2917101543 @default.
- W2966964169 cites W2936080613 @default.
- W2966964169 cites W2945191715 @default.
- W2966964169 cites W3003427357 @default.
- W2966964169 cites W4241316979 @default.
- W2966964169 cites W613111675 @default.
- W2966964169 cites W2116100017 @default.
- W2966964169 doi "https://doi.org/10.1371/journal.pone.0220416" @default.
- W2966964169 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6697330" @default.
- W2966964169 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31419228" @default.
- W2966964169 hasPublicationYear "2019" @default.