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- W2967128530 abstract "Time-resolved fluorescence is a widely adopted technique for DNA detection due to its high sensitivity and selectivity. However, due to stringent requirements on optics and electronics, instrumentation with time-resolved capability is bulky and expensive, prohibiting their use in portable and point-of-care applications. In this work, a fully-integrated DNA biosensor based on liquid-core waveguide (LCW) optics for fluorescence lifetime analysis is presented. The DNA biosensor encompasses all-custom bioassay, optics and electronics into a microsystem, delivering a near sample-to-answer level of integration. Lifetime, rather than intensity, is exploited as the analytical signal from the V-carbazole probe for the first time. Excitation propagation within the LCW is investigated both analytically and in simulations to achieve high excitation rejection and low temporal dispersion, enabling the proposed LCW-based system with much smaller instrumentation size to deliver comparable lifetime measurement accuracy to traditional systems. Detection of DNA down to 15 base pairs at a low detection limit of 1.38 nM demonstrates the high applicability of the proposed biosensor for compact, application-specific, and low-cost diagnostics devices." @default.
- W2967128530 created "2019-08-22" @default.
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- W2967128530 date "2019-01-01" @default.
- W2967128530 modified "2023-09-24" @default.
- W2967128530 title "Fully Integrated Liquid-Core Waveguide Fluorescence Lifetime Detection Microsystem for DNA Biosensing" @default.
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- W2967128530 doi "https://doi.org/10.1109/access.2019.2934764" @default.
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