FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2967369839> ?p ?o ?g. }

• W2967369839 abstract "Ubiquitin E3 ligase CUL4A plays important oncogenic roles in the development of cancers. DTL, one of the CUL4-DDB1 associated factors (DCAFs), may involve in the process of cancer development. Programmed cell death 4 (PDCD4) is a tumor suppressor gene involved in cell apoptosis, transformation, invasion and tumor progression. Affinity-purification mass spectrometry was used to identify potential DTL interaction proteins. Co-immunoprecipitation (Co-IP) was performed to verify protein interaction between DTL and PDCD4. mRNA levels in cancer cells and tissues were detected by Quantitative real-time PCR. Lentivirus was used to establish stable overexpression and knocking down cell lines for DTL and PDCD4. Transwell and wound healing assays were used to determine migration ability of cancer cells. Matrigel assay was used to determine invasion ability of cancer cells. MTT and colony formation assays were used to evaluate proliferation of cancer cells. In this study, programmed cell death 4 (PDCD4) was identified as a potential substrate of DTL. Co-IP and immunofluorescence assays further confirmed the interaction between DTL and PDCD4. Moreover, DTL overexpression decreased the protein level and accelerated the degradation rate of PDCD4. Through in vitro ubiquitination experiment, we proved that PDCD4 was degraded by DTL through ubiquitination. Clinically DTL was significantly up-regulated in cancer tissues than that in normal tissues. The survival curves showed that cancer patients with higher DTL expression owned lower survival rate. Functional experiments showed that DTL not only enhanced the proliferation and migration abilities of cancer cells, but also promoted the tumorigenesis in nude mice. Rescued experiment results demonstrated that silencing PDCD4 simultaneous with DTL recovered the phenotypes defect caused by DTL knocking down. Our results elucidated that DTL enhanced the motility and proliferation of cancer cells through degrading PDCD4 to promote the development of cancers." @default.
• W2967369839 created "2019-08-22" @default.
• W2967369839 creator A5006847122 @default.
• W2967369839 creator A5020966873 @default.
• W2967369839 creator A5030916695 @default.
• W2967369839 creator A5046892858 @default.
• W2967369839 creator A5047090305 @default.
• W2967369839 creator A5051442571 @default.
• W2967369839 creator A5065243448 @default.
• W2967369839 date "2019-08-13" @default.
• W2967369839 modified "2023-10-16" @default.
• W2967369839 title "DTL promotes cancer progression by PDCD4 ubiquitin-dependent degradation" @default.
• W2967369839 cites W1904404816 @default.
• W2967369839 cites W1973190617 @default.
• W2967369839 cites W1982388210 @default.
• W2967369839 cites W1982855701 @default.
• W2967369839 cites W1990561843 @default.
• W2967369839 cites W1995461329 @default.
• W2967369839 cites W1997202121 @default.
• W2967369839 cites W2014491514 @default.
• W2967369839 cites W2014867464 @default.
• W2967369839 cites W2031189137 @default.
• W2967369839 cites W2042812307 @default.
• W2967369839 cites W2049437111 @default.
• W2967369839 cites W2050079849 @default.
• W2967369839 cites W2053501754 @default.
• W2967369839 cites W2058772642 @default.
• W2967369839 cites W2068784898 @default.
• W2967369839 cites W2069104846 @default.
• W2967369839 cites W2069926873 @default.
• W2967369839 cites W2070433128 @default.
• W2967369839 cites W2074291852 @default.
• W2967369839 cites W2079512206 @default.
• W2967369839 cites W2079859240 @default.
• W2967369839 cites W2090222973 @default.
• W2967369839 cites W2094458603 @default.
• W2967369839 cites W2104812418 @default.
• W2967369839 cites W2106130068 @default.
• W2967369839 cites W2110779321 @default.
• W2967369839 cites W2119760558 @default.
• W2967369839 cites W2120923391 @default.
• W2967369839 cites W2125040419 @default.
• W2967369839 cites W2131941047 @default.
• W2967369839 cites W2146644156 @default.
• W2967369839 cites W2149931676 @default.
• W2967369839 cites W2157437933 @default.
• W2967369839 cites W2159430811 @default.
• W2967369839 cites W2162262059 @default.
• W2967369839 cites W2169301624 @default.
• W2967369839 cites W2170564629 @default.
• W2967369839 cites W2180612428 @default.
• W2967369839 cites W2303909027 @default.
• W2967369839 cites W2310322011 @default.
• W2967369839 cites W2412499442 @default.
• W2967369839 cites W2420376897 @default.
• W2967369839 cites W2531294657 @default.
• W2967369839 cites W2607129810 @default.
• W2967369839 cites W2735435941 @default.
• W2967369839 doi "https://doi.org/10.1186/s13046-019-1358-x" @default.
• W2967369839 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6693180" @default.
• W2967369839 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31409387" @default.
• W2967369839 hasPublicationYear "2019" @default.
• W2967369839 type Work @default.
• W2967369839 sameAs 2967369839 @default.
• W2967369839 citedByCount "57" @default.
• W2967369839 countsByYear W29673698392020 @default.
• W2967369839 countsByYear W29673698392021 @default.
• W2967369839 countsByYear W29673698392022 @default.
• W2967369839 countsByYear W29673698392023 @default.
• W2967369839 crossrefType "journal-article" @default.
• W2967369839 hasAuthorship W2967369839A5006847122 @default.
• W2967369839 hasAuthorship W2967369839A5020966873 @default.
• W2967369839 hasAuthorship W2967369839A5030916695 @default.
• W2967369839 hasAuthorship W2967369839A5046892858 @default.
• W2967369839 hasAuthorship W2967369839A5047090305 @default.
• W2967369839 hasAuthorship W2967369839A5051442571 @default.
• W2967369839 hasAuthorship W2967369839A5065243448 @default.
• W2967369839 hasBestOaLocation W29673698391 @default.
• W2967369839 hasConcept C104317684 @default.
• W2967369839 hasConcept C121608353 @default.
• W2967369839 hasConcept C134459356 @default.
• W2967369839 hasConcept C153911025 @default.
• W2967369839 hasConcept C185592680 @default.
• W2967369839 hasConcept C190283241 @default.
• W2967369839 hasConcept C25602115 @default.
• W2967369839 hasConcept C2778608917 @default.
• W2967369839 hasConcept C2780394083 @default.
• W2967369839 hasConcept C31573885 @default.
• W2967369839 hasConcept C502942594 @default.
• W2967369839 hasConcept C54355233 @default.
• W2967369839 hasConcept C55493867 @default.
• W2967369839 hasConcept C555283112 @default.
• W2967369839 hasConcept C62112901 @default.
• W2967369839 hasConcept C86803240 @default.
• W2967369839 hasConcept C95444343 @default.
• W2967369839 hasConcept C96232424 @default.
• W2967369839 hasConceptScore W2967369839C104317684 @default.
• W2967369839 hasConceptScore W2967369839C121608353 @default.
• W2967369839 hasConceptScore W2967369839C134459356 @default.
• W2967369839 hasConceptScore W2967369839C153911025 @default.

• next