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- W2967372298 abstract "Lipid desaturation plays important roles in biological processes and the disease states. Here, we report a simple but efficient method for mapping unsaturated phospholipids including the spatial distribution of lipid C=C location isomers in animal organs by coupling the C=C specific derivatization with direct analysis mass spectrometry (MS). Lipids are sampled directly by a stainless-steel wire from rat brain or kidney, extracted, and derivatized via the Paternò-Büchi reaction in a glass emitter of the nanoelectrospray ionization (nanoESI) source. Subsequent analysis by nanoESI-tandem mass spectrometry reveals C=C locations and relative quantities of lipid C=C location isomers. Unsaturated lipids, such as phospholipids and free fatty acids, have been identified with ion intensities spanning two orders of magnitude in rat brain. Typical sample consumption is less than 10 μg/measurement and the time for each analysis is about 3 min. This method should serve as a complementary method to high spatial resolution mass spectrometry imaging techniques, because it offers a streamlined experimental workflow for rapid profiling of lipids with C=C specificity to enable such applications as point-of-care disease diagnostics." @default.
- W2967372298 created "2019-08-22" @default.
- W2967372298 creator A5010877617 @default.
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- W2967372298 date "2019-11-01" @default.
- W2967372298 modified "2023-10-16" @default.
- W2967372298 title "Mapping lipid C=C location isomers in organ tissues by coupling photochemical derivatization and rapid extractive mass spectrometry" @default.
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- W2967372298 doi "https://doi.org/10.1016/j.ijms.2019.116206" @default.
- W2967372298 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7133712" @default.
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- W2967372298 hasPublicationYear "2019" @default.
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