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• W2967528979 abstract "Objective: This study aimed to investigate if β-cyclodextrin nanoparticles potentiate the photodynamic antimicrobial chemotherapy (PACT) effects in single and microcosm oral biofilms using methylene blue (MB) and a red laser. Background data: Studies of PACT have demonstrated promising effects; however, the association of nanoparticles with photosensitizers could enhance the antimicrobial result. Materials and methods: Biofilms were grown on enamel blocks either with Streptococcus mutans or in a microcosm model (salivary microorganisms) supplemented with sucrose. PACT using 50 μM MB associated or not with 32 μM encapsulated β-cyclodextrin with MB for 5 min, followed by irradiation with red laser (λ = 660 nm, 320 J/cm2), was conducted and the counts of viable microorganisms in proper selective media were determined. Data were analyzed by one-factor ANOVA, followed by Tukey's test, or Kruskal–Wallis test, followed by Dunn's post hoc test, all with a significance level of 5%. Results: In the single-species biofilm model, a significant reduction in S. mutans counts was found for all groups when light was present. In the microcosm biofilm model, no significant difference was found among the groups for total streptococci, but a significant reduction of S. mutans was observed for the PACT group of encapsulated β-cyclodextrin+MB. However, no statistically significant difference was observed among the PACT groups. Conclusions: PACT with β-cyclodextrin mediated with MB associated with a red laser reduced S. mutans in microcosm biofilms. However, the presence of β-cyclodextrin nanoparticles did not potentiate the PACT effects in single or microcosm oral biofilms." @default.
• W2967528979 created "2019-08-22" @default.
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• W2967528979 date "2019-09-01" @default.
• W2967528979 modified "2023-10-01" @default.
• W2967528979 title "Combined Effectiveness of β-Cyclodextrin Nanoparticles in Photodynamic Antimicrobial Chemotherapy on <i>In Vitro</i> Oral Biofilms" @default.
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• W2967528979 doi "https://doi.org/10.1089/photob.2019.4669" @default.
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