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• W2968220354 abstract "Abstract Objective After myocardial infarction, neutrophils rapidly and massively infiltrate the heart, where they can promote both tissue healing and damage. Here, we investigated the dynamics of cardiac neutrophil heterogeneity after infarction. Methods and results We employed single-cell transcriptomics (scRNA-seq) to investigate temporal neutrophil heterogeneity in the heart after murine myocardial infarction. At day 1, 3, and 5 after infarction, neutrophils could be delineated into six distinct clusters with specific time-dependent patterning and proportions. While the majority of neutrophils at day 1 were characterized by high expression of chemokines (e.g. Cxcl3 , Ccl6 ), and putative activity of transcriptional regulators involved in hypoxic response ( Hif1a ) and emergency granulopoiesis ( Cebpb ), two major subsets of Siglecf hi (enriched for e.g. Icam1 and Tnf ) and Siglecf low ( Slpi, Ifitm1 ) neutrophils were found at 3 and 5 days. Flow cytometry analysis confirmed the presence of LY6G + SIGLECF hi and LY6G + SIGLECF low neutrophils in the heart from 3 days after infarction onwards. LY6G + SIGLECF hi neutrophils were absent from the bone marrow, blood and spleen, suggesting local acquisition of surface SIGLECF. Acquisition of the SIGLECF hi state was paralleled by features of neutrophil ageing and activation (ICAM1 hi CXCR4 hi CD49d hi CD62L low ). scRNA-seq of atherosclerotic aortas revealed two neutrophil subsets with gene expression patterns reminiscent of the major Siglecf hi and Siglecf low cardiac neutrophil subpopulations, revealing that these populations may be present across distinct contexts of cardiovascular inflammation. Conclusion Altogether, our data provide a time-resolved census of neutrophil diversity and gene expression dynamics in the mouse ischemic heart at the single-cell level, and suggests that temporal neutrophil heterogeneity is in part driven by local transition to a SIGLECF hi state." @default.
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• W2968220354 date "2019-08-16" @default.
• W2968220354 modified "2023-10-01" @default.
• W2968220354 title "Time-resolved single-cell transcriptomics uncovers dynamics of cardiac neutrophil diversity in murine myocardial infarction" @default.
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• W2968220354 doi "https://doi.org/10.1101/738005" @default.
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