FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2968277001> ?p ?o ?g. }

• W2968277001 endingPage "129414" @default.
• W2968277001 startingPage "129414" @default.
• W2968277001 abstract "Unlike other cadherins, T-cadherin does not mediate strong cell-cell adhesion. It has two soluble ligands: low density lipoprotein (LDL) and high-molecular-weight (HMW) adiponectin. LDL binding to T-cadherin induces calcium signaling, migration, and proliferation, and has proatherogenic effects, but adiponectin binding promotes antiatherogenic effects. The reasons for this difference and mechanism of signal transduction by glycosylphosphatidylinositol (GPI)-anchored T-cadherin are unknown. We compared the ability of LDL and HMW adiponectin to induce calcium signaling, T-cadherin clustering and internalization. We measured calcium signaling in smooth muscle cells and T-cadherin expressing HEK293 using single-cell imaging. To study receptor clustering, we tested three different T-cadherin labeling strategies and then utilized confocal microscopy and flow cytometry assays based on Förster resonance energy transfer (FRET). Enzymatically labeled T-cadherin retained its cellular localization and physiological activity, features that were otherwise affected by fluorescent proteins and antibodies. This labeling method allowed us to study T-cadherin clustering dynamics at the cell surface. HMW adiponectin induced the formation of stable T-cadherin clusters while LDL induced short-lived clusters. Cellular responses were also different: LDL triggered cholesterol- and actin-dependent calcium signaling without internalization while adiponectin promoted the opposite effect. We revealed distinct ligand-specific T-cadherin clustering and its ability to induce internalization or intracellular calcium signaling that likely explains the different physiological effects of LDL and HMW adiponectin. This work highlights the importance of GPI-anchored receptor clustering dynamics in mediating cellular responses. Different ligands can induce different effects in an identical cell via the same receptor." @default.
• W2968277001 created "2019-08-22" @default.
• W2968277001 creator A5021300992 @default.
• W2968277001 creator A5035482823 @default.
• W2968277001 creator A5057461148 @default.
• W2968277001 creator A5073762498 @default.
• W2968277001 creator A5079214942 @default.
• W2968277001 date "2019-11-01" @default.
• W2968277001 modified "2023-10-01" @default.
• W2968277001 title "Different spatiotemporal organization of GPI-anchored T-cadherin in response to low-density lipoprotein and adiponectin" @default.
• W2968277001 cites W1803102843 @default.
• W2968277001 cites W1970307313 @default.
• W2968277001 cites W1973591364 @default.
• W2968277001 cites W1975765740 @default.
• W2968277001 cites W1975917693 @default.
• W2968277001 cites W1980658440 @default.
• W2968277001 cites W1980926711 @default.
• W2968277001 cites W1980937260 @default.
• W2968277001 cites W1986293963 @default.
• W2968277001 cites W1986849641 @default.
• W2968277001 cites W1988280031 @default.
• W2968277001 cites W1993132387 @default.
• W2968277001 cites W1993717812 @default.
• W2968277001 cites W2000638372 @default.
• W2968277001 cites W2002154523 @default.
• W2968277001 cites W2006689028 @default.
• W2968277001 cites W2009009400 @default.
• W2968277001 cites W2012470225 @default.
• W2968277001 cites W2016421144 @default.
• W2968277001 cites W2019351415 @default.
• W2968277001 cites W2020719323 @default.
• W2968277001 cites W2023993671 @default.
• W2968277001 cites W2024692900 @default.
• W2968277001 cites W2024700679 @default.
• W2968277001 cites W2031258536 @default.
• W2968277001 cites W2037584275 @default.
• W2968277001 cites W2039840864 @default.
• W2968277001 cites W2049832267 @default.
• W2968277001 cites W2050520078 @default.
• W2968277001 cites W2053718569 @default.
• W2968277001 cites W2054551128 @default.
• W2968277001 cites W2055843695 @default.
• W2968277001 cites W2057635048 @default.
• W2968277001 cites W2058652637 @default.
• W2968277001 cites W2060259273 @default.
• W2968277001 cites W2062409614 @default.
• W2968277001 cites W2073393757 @default.
• W2968277001 cites W2082673878 @default.
• W2968277001 cites W2086069231 @default.
• W2968277001 cites W2087778160 @default.
• W2968277001 cites W2092150552 @default.
• W2968277001 cites W2092164532 @default.
• W2968277001 cites W2094325380 @default.
• W2968277001 cites W2095022880 @default.
• W2968277001 cites W2100837269 @default.
• W2968277001 cites W2101048587 @default.
• W2968277001 cites W2104125356 @default.
• W2968277001 cites W2111116814 @default.
• W2968277001 cites W2112220590 @default.
• W2968277001 cites W2124974684 @default.
• W2968277001 cites W2125510758 @default.
• W2968277001 cites W2128671739 @default.
• W2968277001 cites W2131970139 @default.
• W2968277001 cites W2132629607 @default.
• W2968277001 cites W2132759053 @default.
• W2968277001 cites W2135503869 @default.
• W2968277001 cites W2135966071 @default.
• W2968277001 cites W2137960852 @default.
• W2968277001 cites W2138742704 @default.
• W2968277001 cites W2141260882 @default.
• W2968277001 cites W2141708801 @default.
• W2968277001 cites W2144997725 @default.
• W2968277001 cites W2146255300 @default.
• W2968277001 cites W2150881786 @default.
• W2968277001 cites W2154298653 @default.
• W2968277001 cites W2156799866 @default.
• W2968277001 cites W2160670356 @default.
• W2968277001 cites W2168761911 @default.
• W2968277001 cites W2170886970 @default.
• W2968277001 cites W2171183814 @default.
• W2968277001 cites W2225796847 @default.
• W2968277001 cites W2321608341 @default.
• W2968277001 cites W2328394317 @default.
• W2968277001 cites W2409732953 @default.
• W2968277001 cites W2431621945 @default.
• W2968277001 cites W2570064302 @default.
• W2968277001 cites W2581827255 @default.
• W2968277001 cites W2599649240 @default.
• W2968277001 cites W2602367518 @default.
• W2968277001 cites W2765179709 @default.
• W2968277001 cites W2778242679 @default.
• W2968277001 cites W2801615956 @default.
• W2968277001 cites W2883072970 @default.
• W2968277001 cites W4246437849 @default.
• W2968277001 doi "https://doi.org/10.1016/j.bbagen.2019.129414" @default.
• W2968277001 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31404618" @default.
• W2968277001 hasPublicationYear "2019" @default.
• W2968277001 type Work @default.

• next