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- W2968401610 abstract "Cloning VH and VL genes from individual antigen-specific B cells is an attractive approach for producing monoclonal antibodies of the desired specificity. Current RT-PCR protocols, however, result in the successful identification of VH and VL gene pairs in about half of the sorted cells. Here, we demonstrate that single-cell RT-PCR is likely affected by stochastic factors, and that running PCRs in triplicate results in successful amplification of the expressed VH and VL genes in 90–100% of single sorted human B cells." @default.
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- W2968401610 date "2019-10-01" @default.
- W2968401610 modified "2023-09-26" @default.
- W2968401610 title "A simple way to increase recovery of the expressed <i>VH</i> and <i>VL</i> genes in single-sorted human B cells" @default.
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- W2968401610 doi "https://doi.org/10.2144/btn-2019-0079" @default.
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