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- W2968681844 abstract "Abstract Cryptococcosis is a fungal disease caused by C . neoformans . To adapt and survive in diverse ecological niches, including the animal host, this opportunistic pathogen relies on its ability to uptake nutrients, such as carbon, nitrogen, iron, phosphate, sulfur, and amino acids. Genetic circuits play a role in the response to environmental changes, modulating gene expression and adjusting the microbial metabolism to the nutrients available for the best energy usage and survival. We studied the sulfur amino acid biosynthesis and its implications on C . neoformans biology and virulence. CNAG_04798 encodes a BZip protein and was annotated as CYS3 , which has been considered an essential gene. However, we demonstrated that CYS3 is not essential, in fact, its knockout led to sulfur amino acids auxotroph. Western blots and fluorescence microscopy indicated that GFP-Cys3, which is expressed from a constitutive promoter, localizes to the nucleus in rich medium (YEPD); the addition of methionine and cysteine as sole nitrogen source (SD–N + Met/Cys) led to reduced nuclear localization and protein degradation. By proteomics, we identified and confirmed physical interaction among Gpp2, Cna1, Cnb1 and GFP-Cys3. Deletion of the calcineurin and GPP2 genes in a GFP-Cys3 background demonstrated that calcineurin is required to maintain Cys3 high protein levels in YEPD and that deletion of GPP2 causes GFP-Cys3 to persist in the presence of sulfur amino acids. Global transcriptional profile of mutant and wild type by RNAseq revealed that Cys3 controls all branches of the sulfur amino acid biosynthesis, and sulfur starvation leads to induction of several amino acid biosynthetic routes. In addition, we found that Cys3 is required for virulence in Galleria mellonella animal model." @default.
- W2968681844 created "2019-08-22" @default.
- W2968681844 creator A5027516009 @default.
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- W2968681844 date "2019-08-15" @default.
- W2968681844 modified "2023-10-01" @default.
- W2968681844 title "The regulation of the sulfur amino acid biosynthetic pathway in Cryptococcus neoformans: the relationship of Cys3, Calcineurin, and Gpp2 phosphatases" @default.
- W2968681844 cites W1535070510 @default.
- W2968681844 cites W1537801754 @default.
- W2968681844 cites W1551144904 @default.
- W2968681844 cites W1583172410 @default.
- W2968681844 cites W1809656640 @default.
- W2968681844 cites W1826309415 @default.
- W2968681844 cites W1872803163 @default.
- W2968681844 cites W1901882589 @default.
- W2968681844 cites W1925046267 @default.
- W2968681844 cites W1929130243 @default.
- W2968681844 cites W1966848901 @default.
- W2968681844 cites W1968672066 @default.
- W2968681844 cites W1973736583 @default.
- W2968681844 cites W1973996060 @default.
- W2968681844 cites W1981239770 @default.
- W2968681844 cites W1982174478 @default.
- W2968681844 cites W1983621788 @default.
- W2968681844 cites W1995716972 @default.
- W2968681844 cites W1996783999 @default.
- W2968681844 cites W2001275376 @default.
- W2968681844 cites W2005800004 @default.
- W2968681844 cites W2010445962 @default.
- W2968681844 cites W2014931848 @default.
- W2968681844 cites W2019751056 @default.
- W2968681844 cites W2022406795 @default.
- W2968681844 cites W2033593008 @default.
- W2968681844 cites W2036170363 @default.
- W2968681844 cites W2039330325 @default.
- W2968681844 cites W2041361121 @default.
- W2968681844 cites W2047879517 @default.
- W2968681844 cites W2049713754 @default.
- W2968681844 cites W2055203777 @default.
- W2968681844 cites W2062222285 @default.
- W2968681844 cites W2062709789 @default.
- W2968681844 cites W2067346920 @default.
- W2968681844 cites W2070218828 @default.
- W2968681844 cites W2072054841 @default.
- W2968681844 cites W2086229455 @default.
- W2968681844 cites W2086413957 @default.
- W2968681844 cites W2089394802 @default.
- W2968681844 cites W2090904748 @default.
- W2968681844 cites W2092724483 @default.
- W2968681844 cites W2092931292 @default.
- W2968681844 cites W2094041961 @default.
- W2968681844 cites W2097390727 @default.
- W2968681844 cites W2097467142 @default.
- W2968681844 cites W2103485717 @default.
- W2968681844 cites W2104047392 @default.
- W2968681844 cites W2107277218 @default.
- W2968681844 cites W2110600759 @default.
- W2968681844 cites W2111030334 @default.
- W2968681844 cites W2119178613 @default.
- W2968681844 cites W2119727332 @default.
- W2968681844 cites W2121749642 @default.
- W2968681844 cites W2123610090 @default.
- W2968681844 cites W2126373302 @default.
- W2968681844 cites W2131271579 @default.
- W2968681844 cites W2134526812 @default.
- W2968681844 cites W2134624875 @default.
- W2968681844 cites W2136651521 @default.
- W2968681844 cites W2138255346 @default.
- W2968681844 cites W2141224543 @default.
- W2968681844 cites W2144353353 @default.
- W2968681844 cites W2145966324 @default.
- W2968681844 cites W2148376015 @default.
- W2968681844 cites W2157644081 @default.
- W2968681844 cites W2159227723 @default.
- W2968681844 cites W2161188281 @default.
- W2968681844 cites W2162294247 @default.
- W2968681844 cites W2170409189 @default.
- W2968681844 cites W2179438025 @default.
- W2968681844 cites W2407688173 @default.
- W2968681844 cites W2497375665 @default.
- W2968681844 cites W2511161664 @default.
- W2968681844 cites W2528943852 @default.
- W2968681844 cites W2537623931 @default.
- W2968681844 cites W2559476747 @default.
- W2968681844 cites W2604923038 @default.
- W2968681844 cites W2740199405 @default.
- W2968681844 cites W2758656048 @default.
- W2968681844 cites W2768594611 @default.
- W2968681844 cites W2769768822 @default.
- W2968681844 cites W2796999830 @default.
- W2968681844 cites W2800236749 @default.
- W2968681844 cites W2900532468 @default.