FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2968698545> ?p ?o ?g. }

• W2968698545 endingPage "5182" @default.
• W2968698545 startingPage "5182" @default.
• W2968698545 abstract "Background: Epigenetic alterations are common in many cancer types and play an important role in their pathogenesis. However, the role of aberrant DNA methylation in Ewing Sarcoma (ES), a malignant neoplasm of bone and soft tissues in adolescents and young adults, is currently poorly understood. Aim: To determine the role of aberrant DNA methylation in the molecular pathology of ES Methods: We used a genome wide approach to identify genes hypermethylated in these neoplasms. Genomic DNA was isolated from formalin fixed, paraffin embedded primary ES tumors and cell lines. We compared the methylation signatures of 13 tumors and 3 ES cell lines with human mesenchymal stem cell primary cultures (hMSCs). Illumina Goldengate methylation analysis was used to determine the methylation status of 800 genes known to be altered in human cancer. Validation of the array results was performed with methylation specific PCR and sodium bisulfite sequencing. Results: The Illumina Goldengate arrays were found to be both sensitive and reproducible. After filtering out genes methylated in hMSCs, we determined that primary ES tumors and cell lines could be stratified into low and high methylator groups. In addition, we identified a group of genes that were methylated in >30% of ES primary tumors (range 30-100%) but not in hMSCs. Known tumor suppressor genes and genes methylated in other cancers were included in this group, including RASSF1A, a known tumor suppressor gene methylated in both adult and pediatric cancers. Methylation specific PCR and bisulfite sequencing analysis of RASSF1A confirmed the aberrant methylation detected by the methylation arrays in the ES cell lines. Furthermore, treatment of the cell lines with 5-aza-2#8217;deoxycytidine induced RASSF1A expression in the cell lines with methylated RASSF1A demonstrating that RASSF1A is epigenetically silenced in ES cell lines due to DNA hypermethylation. Conclusions: Aberrant DNA methylation occurs in ES, demonstrating that epigenetic alterations play a role in pediatric tumors of mesenchymal origin. Determination of the genes affected by this process will provide further insights into the molecular pathology of ES. Citation Information: In: Proc Am Assoc Cancer Res; 2009 Apr 18-22; Denver, CO. Philadelphia (PA): AACR; 2009. Abstract nr 5182." @default.
• W2968698545 created "2019-08-22" @default.
• W2968698545 creator A5010962133 @default.
• W2968698545 creator A5026990573 @default.
• W2968698545 creator A5068477057 @default.
• W2968698545 creator A5078492954 @default.
• W2968698545 date "2009-05-01" @default.
• W2968698545 modified "2023-09-27" @default.
• W2968698545 title "Abstract #5182: Genome wide assessment of epigenetic alterations in Ewing Sarcoma" @default.
• W2968698545 hasPublicationYear "2009" @default.
• W2968698545 type Work @default.
• W2968698545 sameAs 2968698545 @default.
• W2968698545 citedByCount "0" @default.
• W2968698545 crossrefType "journal-article" @default.
• W2968698545 hasAuthorship W2968698545A5010962133 @default.
• W2968698545 hasAuthorship W2968698545A5026990573 @default.
• W2968698545 hasAuthorship W2968698545A5068477057 @default.
• W2968698545 hasAuthorship W2968698545A5078492954 @default.
• W2968698545 hasConcept C104317684 @default.
• W2968698545 hasConcept C150194340 @default.
• W2968698545 hasConcept C153911025 @default.
• W2968698545 hasConcept C189235521 @default.
• W2968698545 hasConcept C190727270 @default.
• W2968698545 hasConcept C33288867 @default.
• W2968698545 hasConcept C41091548 @default.
• W2968698545 hasConcept C502942594 @default.
• W2968698545 hasConcept C54355233 @default.
• W2968698545 hasConcept C86803240 @default.
• W2968698545 hasConceptScore W2968698545C104317684 @default.
• W2968698545 hasConceptScore W2968698545C150194340 @default.
• W2968698545 hasConceptScore W2968698545C153911025 @default.
• W2968698545 hasConceptScore W2968698545C189235521 @default.
• W2968698545 hasConceptScore W2968698545C190727270 @default.
• W2968698545 hasConceptScore W2968698545C33288867 @default.
• W2968698545 hasConceptScore W2968698545C41091548 @default.
• W2968698545 hasConceptScore W2968698545C502942594 @default.
• W2968698545 hasConceptScore W2968698545C54355233 @default.
• W2968698545 hasConceptScore W2968698545C86803240 @default.
• W2968698545 hasLocation W29686985451 @default.
• W2968698545 hasOpenAccess W2968698545 @default.
• W2968698545 hasPrimaryLocation W29686985451 @default.
• W2968698545 hasRelatedWork W1869577024 @default.
• W2968698545 hasRelatedWork W1975760059 @default.
• W2968698545 hasRelatedWork W1980799670 @default.
• W2968698545 hasRelatedWork W1987032884 @default.
• W2968698545 hasRelatedWork W2016523708 @default.
• W2968698545 hasRelatedWork W2033735604 @default.
• W2968698545 hasRelatedWork W2057311053 @default.
• W2968698545 hasRelatedWork W2091783958 @default.
• W2968698545 hasRelatedWork W2132119564 @default.
• W2968698545 hasRelatedWork W2133700934 @default.
• W2968698545 hasRelatedWork W2147511945 @default.
• W2968698545 hasRelatedWork W2227478911 @default.
• W2968698545 hasRelatedWork W2398546321 @default.
• W2968698545 hasRelatedWork W2555914695 @default.
• W2968698545 hasRelatedWork W2619972249 @default.
• W2968698545 hasRelatedWork W2804444954 @default.
• W2968698545 hasRelatedWork W2955913131 @default.
• W2968698545 hasRelatedWork W3037047062 @default.
• W2968698545 hasRelatedWork W3210819990 @default.
• W2968698545 hasRelatedWork W2799294244 @default.
• W2968698545 hasVolume "69" @default.
• W2968698545 isParatext "false" @default.
• W2968698545 isRetracted "false" @default.
• W2968698545 magId "2968698545" @default.
• W2968698545 workType "article" @default.